Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections

Venkatesh, Nandhitha; Koss, Max J.; Greco, Claudio; Nickles, Grant; Wiemann, Philipp; Keller, Nancy P.

doi:10.3390/microorganisms9102123

Journal article 597 views 80 downloads

Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections

Nandhitha Venkatesh, Max J. Koss, Claudio Greco

, Grant Nickles

, Philipp Wiemann, Nancy P. Keller

Microorganisms, Volume: 9, Issue: 10, Start page: 2123

Swansea University Author: Claudio Greco

PDF | Version of Record

© 2021 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license
Download (4.32MB)

Check full text

DOI (Published version): 10.3390/microorganisms9102123

Abstract

In order to gain a comprehensive understanding of plant disease in natural and agricultural ecosystems, it is essential to examine plant disease in multi-pathogen–host systems. Ralstonia solanacearum and Fusarium oxysporum f. sp. lycopersici are vascular wilt pathogens that can result in heavy yield...

Full description

Published in:	Microorganisms
ISSN:	2076-2607
Published:	MDPI AG 2021
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa61512

first_indexed	2022-10-20T12:48:19Z
last_indexed	2023-01-13T19:22:18Z
id	cronfa61512
recordtype	SURis
fullrecord	<?xml version="1.0"?><rfc1807><datestamp>2022-10-20T13:49:51.8967349</datestamp><bib-version>v2</bib-version><id>61512</id><entry>2022-10-10</entry><title>Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections</title><swanseaauthors><author><sid>cacac6459bd7cf4a241f63661006036f</sid><ORCID>0000-0003-3067-0999</ORCID><firstname>Claudio</firstname><surname>Greco</surname><name>Claudio Greco</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2022-10-10</date><deptcode>BGPS</deptcode><abstract>In order to gain a comprehensive understanding of plant disease in natural and agricultural ecosystems, it is essential to examine plant disease in multi-pathogen–host systems. Ralstonia solanacearum and Fusarium oxysporum f. sp. lycopersici are vascular wilt pathogens that can result in heavy yield losses in susceptible hosts such as tomato. Although both pathogens occupy the xylem, the costs of mixed infections on wilt disease are unknown. Here, we characterize the consequences of co-infection with R. solanacearum and F. oxysporum using tomato as the model host. Our results demonstrate that bacterial wilt severity is reduced in co-infections, that bikaverin synthesis by Fusarium contributes to bacterial wilt reduction, and that the arrival time of each microbe at the infection court is important in driving the severity of wilt disease. Further, analysis of the co-infection root secretome identified previously uncharacterized secreted metabolites that reduce R. solanacearum growth in vitro and provide protection to tomato seedlings against bacterial wilt disease. Taken together, these results highlight the need to understand the consequences of mixed infections in plant disease.</abstract><type>Journal Article</type><journal>Microorganisms</journal><volume>9</volume><journalNumber>10</journalNumber><paginationStart>2123</paginationStart><paginationEnd/><publisher>MDPI AG</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>2076-2607</issnElectronic><keywords>secondary metabolites; plant–microbe interactions; coinfection; wilt disease; bacterial–fungal interactions; Fusarium oxysporum; Ralstonia solanacearum</keywords><publishedDay>9</publishedDay><publishedMonth>10</publishedMonth><publishedYear>2021</publishedYear><publishedDate>2021-10-09</publishedDate><doi>10.3390/microorganisms9102123</doi><url/><notes/><college>COLLEGE NANME</college><department>Biosciences Geography and Physics School</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BGPS</DepartmentCode><institution>Swansea University</institution><apcterm/><funders>This work was supported by the National Institute of Food and Agriculture, United States Department of Agriculture, Hatch project 1012878 to N.P.K, the National Institutes of Health under grant 5R01GM112739-06 to N.P.K. and the UW-Madison Food Research Institute’s E. Michael and Winona Foster Wisconsin Distinguished Fellowship Award to N.V.</funders><projectreference/><lastEdited>2022-10-20T13:49:51.8967349</lastEdited><Created>2022-10-10T17:22:53.3802042</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Biosciences, Geography and Physics - Biosciences</level></path><authors><author><firstname>Nandhitha</firstname><surname>Venkatesh</surname><order>1</order></author><author><firstname>Max J.</firstname><surname>Koss</surname><order>2</order></author><author><firstname>Claudio</firstname><surname>Greco</surname><orcid>0000-0003-3067-0999</orcid><order>3</order></author><author><firstname>Grant</firstname><surname>Nickles</surname><orcid>0000-0002-0234-8049</orcid><order>4</order></author><author><firstname>Philipp</firstname><surname>Wiemann</surname><order>5</order></author><author><firstname>Nancy P.</firstname><surname>Keller</surname><orcid>0000-0002-4386-9473</orcid><order>6</order></author></authors><documents><document><filename>61512__25527__8813886d738c4b7fb40d13777d57a9a3.pdf</filename><originalFilename>61512_VoR.pdf</originalFilename><uploaded>2022-10-20T13:48:48.6893292</uploaded><type>Output</type><contentLength>4527464</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>© 2021 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>https://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling	2022-10-20T13:49:51.8967349 v2 61512 2022-10-10 Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections cacac6459bd7cf4a241f63661006036f 0000-0003-3067-0999 Claudio Greco Claudio Greco true false 2022-10-10 BGPS In order to gain a comprehensive understanding of plant disease in natural and agricultural ecosystems, it is essential to examine plant disease in multi-pathogen–host systems. Ralstonia solanacearum and Fusarium oxysporum f. sp. lycopersici are vascular wilt pathogens that can result in heavy yield losses in susceptible hosts such as tomato. Although both pathogens occupy the xylem, the costs of mixed infections on wilt disease are unknown. Here, we characterize the consequences of co-infection with R. solanacearum and F. oxysporum using tomato as the model host. Our results demonstrate that bacterial wilt severity is reduced in co-infections, that bikaverin synthesis by Fusarium contributes to bacterial wilt reduction, and that the arrival time of each microbe at the infection court is important in driving the severity of wilt disease. Further, analysis of the co-infection root secretome identified previously uncharacterized secreted metabolites that reduce R. solanacearum growth in vitro and provide protection to tomato seedlings against bacterial wilt disease. Taken together, these results highlight the need to understand the consequences of mixed infections in plant disease. Journal Article Microorganisms 9 10 2123 MDPI AG 2076-2607 secondary metabolites; plant–microbe interactions; coinfection; wilt disease; bacterial–fungal interactions; Fusarium oxysporum; Ralstonia solanacearum 9 10 2021 2021-10-09 10.3390/microorganisms9102123 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University This work was supported by the National Institute of Food and Agriculture, United States Department of Agriculture, Hatch project 1012878 to N.P.K, the National Institutes of Health under grant 5R01GM112739-06 to N.P.K. and the UW-Madison Food Research Institute’s E. Michael and Winona Foster Wisconsin Distinguished Fellowship Award to N.V. 2022-10-20T13:49:51.8967349 2022-10-10T17:22:53.3802042 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Nandhitha Venkatesh 1 Max J. Koss 2 Claudio Greco 0000-0003-3067-0999 3 Grant Nickles 0000-0002-0234-8049 4 Philipp Wiemann 5 Nancy P. Keller 0000-0002-4386-9473 6 61512__25527__8813886d738c4b7fb40d13777d57a9a3.pdf 61512_VoR.pdf 2022-10-20T13:48:48.6893292 Output 4527464 application/pdf Version of Record true © 2021 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license true eng https://creativecommons.org/licenses/by/4.0/
title	Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections
spellingShingle	Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections Claudio Greco
title_short	Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections
title_full	Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections
title_fullStr	Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections
title_full_unstemmed	Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections
title_sort	Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections
author_id_str_mv	cacac6459bd7cf4a241f63661006036f
author_id_fullname_str_mv	cacac6459bd7cf4a241f63661006036f_***_Claudio Greco
author	Claudio Greco
author2	Nandhitha Venkatesh Max J. Koss Claudio Greco Grant Nickles Philipp Wiemann Nancy P. Keller
format	Journal article
container_title	Microorganisms
container_volume	9
container_issue	10
container_start_page	2123
publishDate	2021
institution	Swansea University
issn	2076-2607
doi_str_mv	10.3390/microorganisms9102123
publisher	MDPI AG
college_str	Faculty of Science and Engineering
hierarchytype
hierarchy_top_id	facultyofscienceandengineering
hierarchy_top_title	Faculty of Science and Engineering
hierarchy_parent_id	facultyofscienceandengineering
hierarchy_parent_title	Faculty of Science and Engineering
department_str	School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str	1
active_str	0
description	In order to gain a comprehensive understanding of plant disease in natural and agricultural ecosystems, it is essential to examine plant disease in multi-pathogen–host systems. Ralstonia solanacearum and Fusarium oxysporum f. sp. lycopersici are vascular wilt pathogens that can result in heavy yield losses in susceptible hosts such as tomato. Although both pathogens occupy the xylem, the costs of mixed infections on wilt disease are unknown. Here, we characterize the consequences of co-infection with R. solanacearum and F. oxysporum using tomato as the model host. Our results demonstrate that bacterial wilt severity is reduced in co-infections, that bikaverin synthesis by Fusarium contributes to bacterial wilt reduction, and that the arrival time of each microbe at the infection court is important in driving the severity of wilt disease. Further, analysis of the co-infection root secretome identified previously uncharacterized secreted metabolites that reduce R. solanacearum growth in vitro and provide protection to tomato seedlings against bacterial wilt disease. Taken together, these results highlight the need to understand the consequences of mixed infections in plant disease.
published_date	2021-10-09T14:24:21Z
_version_	1821415793201512448
score	11.247077

Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections

Similar Items