Journal article 169 views 30 downloads
A Rapid Graphene Sensor Platform for the Detection of Viral Proteins in Low Volume Samples
,
Gregory Burwell ,
JACOB MITCHELL,
Muhammad Ali,
Ehsaneh Daghigh Ahmadi,
Bernard Mostert ,
Cerys A. Jenkins ,
Sergiy Rozhko,
Olga Kazakova,
Owen Guy
Advanced NanoBiomed Research, Volume: 2, Issue: 6
Swansea University Authors:
Ffion Walters , Gregory Burwell , JACOB MITCHELL, Muhammad Ali, Ehsaneh Daghigh Ahmadi, Bernard Mostert , Cerys A. Jenkins , Owen Guy
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DOI (Published version): 10.1002/anbr.202100140
Abstract
Infectious disease outbreaks remain an ever-prevalent global issue. The associated demand for rapid diagnostics and onsite testing will play an increasing and critical role in disease surveillance, prevention of the spread of infection, as well as timely commencement of treatment. Reported here is a...
| Published in: | Advanced NanoBiomed Research |
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| ISSN: | 2699-9307 2699-9307 |
| Published: |
Wiley
2022
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa64651 |
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| Abstract: |
Infectious disease outbreaks remain an ever-prevalent global issue. The associated demand for rapid diagnostics and onsite testing will play an increasing and critical role in disease surveillance, prevention of the spread of infection, as well as timely commencement of treatment. Reported here is a graphene–gold nanoparticle hybrid sensor platform technology that is demonstrated for the real-time detection of viral proteins utilizing low volume samples (5 μL). Hepatitis C virus (HCV) is still an endemic problem worldwide and is used as an exemplar system here to demonstrate the capability of the platform viral detection sensor technology. Hepatitis C virus core antigen (HCVcAg) is a promising marker for point-of-care (POC) diagnostic testing for active HCV infection, with the potential to provide a one-stop diagnosis and trigger for the commencement of treatment. Real-time electrical resistance measurements are performed using various concentrations of HCVcAg with linear concentration dependence of resistance on HCVcAg concentration over the range of 100–750 pg mL−1. |
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| Keywords: |
Chemiresistor, gold nanoparticles, graphene biosensor, hepatitis C virus core antigen (HCVcAg), hybrid, real-time |
| College: |
Faculty of Science and Engineering |
| Funders: |
F.W. and G.B. contributed equally to this work. This research was funded by Innovate UK under Newton Fund—China—UK Research and Innovation Bridges Competition 2015 (Project ref.: 102877), Engineering and Physical sciences Research Council (Project ref.: EP/M006301/1), and Knowledge Economy Skills Scholarships (KESS). NPL acknowledges the support of the UK Government Department for Business, Energy and Industrial Strategy through the UK National Quantum Technologies Programme and EU Graphene Flagship under grant agreement GrapheneCore3 881603. The authors also acknowledge support from Avenues of Commercialisation of Nano & Micro Technologies (ACNM) Operation funded by the European Regional Development Fund via the Welsh Government. G.B. acknowledges funding from the Welsh Government Capacity Builder Accelerator Program through the European Regional Development Fund, Welsh European Funding Office, and Swansea University Strategic Initiative in Sustainable Advanced Materials. A.B.M. is a Sêr Cymru II fellow and the results incorporated in this work are supported by the Welsh Government through the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska Curie grant agreement no. 663830. The authors also acknowledge the Application Specific Semiconductor Etch Technologies (ASSET) Project funded by the European Regional Development Fund via the Welsh Governments Smart Expertise Operation. J.J.M. acknowledges the support of the Knowledge Transfer Partnership Associate (Project number 011971) funded by Innovate UK and SPTS Technologies Ltd. Graphene device fabrication and passivation aided by the Centre for NanoHealth technical team. The authors would like to acknowledge Pegasus Chemicals Ltd. for the supply of chemicals used in MVD passivation. The authors would also like to acknowledge Biovici Ltd. for use of their “Sensor-Connect” technology for real-time resistance measurements. The authors would like to thank Mr. Thomas Chess for SEM imaging. |
| Issue: |
6 |