Journal article 1154 views 143 downloads
Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
,
S Rozhko,
D Buckley,
Ehsaneh Daghigh Ahmadi,
Muhammad Ali,
Zari Tehrani ,
Jacob Mitchell,
Gregory Burwell ,
Y Liu,
O Kazakova,
Owen Guy
2D Materials, Volume: 7, Issue: 2, Start page: 024009
Swansea University Authors:
Ffion Walters , Ehsaneh Daghigh Ahmadi, Muhammad Ali, Zari Tehrani , Jacob Mitchell, Gregory Burwell , Owen Guy
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DOI (Published version): 10.1088/2053-1583/ab734c
Abstract
A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π...
| Published in: | 2D Materials |
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| ISSN: | 2053-1583 |
| Published: |
IOP Publishing
2020
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa53518 |
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| Abstract: |
A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π stacking interactions in combination with a ssDNA functionalized AuNP. The ssDNA has complementary sequences which through hybridization, yield the graphene-AuNP hybrid biosensor. Real-time 2-point resistance measurements, performed using varying concentrations of HBsAg, show a linear dependence of resistance change against the logarithm of HBsAg concentration (log[HBsAg]). A limit of detection of 50 pg ml−1 was observed. Moreover, the hybrid biosensor platform has potential to be applied to any biomarker of interest. |
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| Keywords: |
graphene, hepatitis, gold nanoparticle, biosensor, ssDNA, detection, AFM-IR |
| College: |
Faculty of Science and Engineering |
| Issue: |
2 |
| Start Page: |
024009 |