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Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor

Ffion Walters, S Rozhko, D Buckley, Ehsaneh Daghigh Ahmadi, Muhammad Ali, Zari Tehrani Orcid Logo, Jacob Mitchell, Gregory Burwell Orcid Logo, Y Liu, O Kazakova, Owen Guy Orcid Logo

2D Materials, Volume: 7, Issue: 2, Start page: 024009

Swansea University Authors: Ffion Walters, Ehsaneh Daghigh Ahmadi, Muhammad Ali, Zari Tehrani Orcid Logo, Jacob Mitchell, Gregory Burwell Orcid Logo, Owen Guy Orcid Logo

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DOI (Published version): 10.1088/2053-1583/ab734c

Abstract

A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π...

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Published in: 2D Materials
ISSN: 2053-1583
Published: IOP Publishing 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa53518
Abstract: A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π stacking interactions in combination with a ssDNA functionalized AuNP. The ssDNA has complementary sequences which through hybridization, yield the graphene-AuNP hybrid biosensor. Real-time 2-point resistance measurements, performed using varying concentrations of HBsAg, show a linear dependence of resistance change against the logarithm of HBsAg concentration (log[HBsAg]). A limit of detection of 50 pg ml−1 was observed. Moreover, the hybrid biosensor platform has potential to be applied to any biomarker of interest.
Keywords: graphene, hepatitis, gold nanoparticle, biosensor, ssDNA, detection, AFM-IR
College: Faculty of Science and Engineering
Issue: 2
Start Page: 024009

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