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Fungal oxylipins direct programmed developmental switches in filamentous fungi

Mengyao Niu, Breanne N. Steffan, Gregory J. Fischer, Nandhitha Venkatesh, Nicholas L. Raffa, Molly A. Wettstein, Jin Woo Bok, Claudio Greco Orcid Logo, Can Zhao, Erwin Berthier, Ernst Oliw, David Beebe Orcid Logo, Michael Bromley Orcid Logo, Nancy P. Keller Orcid Logo

Nature Communications, Volume: 11, Issue: 1

Swansea University Author: Claudio Greco Orcid Logo

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DOI (Published version): 10.1038/s41467-020-18999-0

Abstract

Filamentous fungi differentiate along complex developmental programs directed by abiotic and biotic signals. Currently, intrinsic signals that govern fungal development remain largely unknown. Here we show that an endogenously produced and secreted fungal oxylipin, 5,8-diHODE, induces fungal cellula...

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Published in: Nature Communications
ISSN: 2041-1723
Published: Springer Science and Business Media LLC 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa61518
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spelling 2022-10-20T13:16:21.9068043 v2 61518 2022-10-10 Fungal oxylipins direct programmed developmental switches in filamentous fungi cacac6459bd7cf4a241f63661006036f 0000-0003-3067-0999 Claudio Greco Claudio Greco true false 2022-10-10 BGPS Filamentous fungi differentiate along complex developmental programs directed by abiotic and biotic signals. Currently, intrinsic signals that govern fungal development remain largely unknown. Here we show that an endogenously produced and secreted fungal oxylipin, 5,8-diHODE, induces fungal cellular differentiation, including lateral branching in pathogenic Aspergillus fumigatus and Aspergillus flavus, and appressorium formation in the rice blast pathogen Magnaporthe grisea. The Aspergillus branching response is specific to a subset of oxylipins and is signaled through G-protein coupled receptors. RNA-Seq profiling shows differential expression of many transcription factors in response to 5,8-diHODE. Screening of null mutants of 33 of those transcription factors identifies three transcriptional regulators that appear to mediate the Aspergillus branching response; one of the mutants is locked in a hypo-branching phenotype, while the other two mutants display a hyper-branching phenotype. Our work reveals an endogenous signal that triggers crucial developmental processes in filamentous fungi, and opens new avenues for research on the morphogenesis of filamentous fungi. Journal Article Nature Communications 11 1 Springer Science and Business Media LLC 2041-1723 14 10 2020 2020-10-14 10.1038/s41467-020-18999-0 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University This study was funded in part by the National Institutes of Health R01 AI065728-01 and GM112739-02 to N.P.K., a Predoctoral Training Program in Genetics award for GF (5T32GM07133), NIH T32 ES007015 to B.N.S., and a Wellcome Trust grant 208396/Z/17/Z to M.B. 2022-10-20T13:16:21.9068043 2022-10-10T17:24:15.6302211 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Mengyao Niu 1 Breanne N. Steffan 2 Gregory J. Fischer 3 Nandhitha Venkatesh 4 Nicholas L. Raffa 5 Molly A. Wettstein 6 Jin Woo Bok 7 Claudio Greco 0000-0003-3067-0999 8 Can Zhao 9 Erwin Berthier 10 Ernst Oliw 11 David Beebe 0000-0002-0415-9006 12 Michael Bromley 0000-0002-7611-0201 13 Nancy P. Keller 0000-0002-4386-9473 14 61518__25521__4d1d2b24ecae4d25a8ee63346b019925.pdf 61518_VoR.pdf 2022-10-20T13:14:33.2576823 Output 2036144 application/pdf Version of Record true © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License true eng http://creativecommons.org/licenses/by/4.0/
title Fungal oxylipins direct programmed developmental switches in filamentous fungi
spellingShingle Fungal oxylipins direct programmed developmental switches in filamentous fungi
Claudio Greco
title_short Fungal oxylipins direct programmed developmental switches in filamentous fungi
title_full Fungal oxylipins direct programmed developmental switches in filamentous fungi
title_fullStr Fungal oxylipins direct programmed developmental switches in filamentous fungi
title_full_unstemmed Fungal oxylipins direct programmed developmental switches in filamentous fungi
title_sort Fungal oxylipins direct programmed developmental switches in filamentous fungi
author_id_str_mv cacac6459bd7cf4a241f63661006036f
author_id_fullname_str_mv cacac6459bd7cf4a241f63661006036f_***_Claudio Greco
author Claudio Greco
author2 Mengyao Niu
Breanne N. Steffan
Gregory J. Fischer
Nandhitha Venkatesh
Nicholas L. Raffa
Molly A. Wettstein
Jin Woo Bok
Claudio Greco
Can Zhao
Erwin Berthier
Ernst Oliw
David Beebe
Michael Bromley
Nancy P. Keller
format Journal article
container_title Nature Communications
container_volume 11
container_issue 1
publishDate 2020
institution Swansea University
issn 2041-1723
doi_str_mv 10.1038/s41467-020-18999-0
publisher Springer Science and Business Media LLC
college_str Faculty of Science and Engineering
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hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
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description Filamentous fungi differentiate along complex developmental programs directed by abiotic and biotic signals. Currently, intrinsic signals that govern fungal development remain largely unknown. Here we show that an endogenously produced and secreted fungal oxylipin, 5,8-diHODE, induces fungal cellular differentiation, including lateral branching in pathogenic Aspergillus fumigatus and Aspergillus flavus, and appressorium formation in the rice blast pathogen Magnaporthe grisea. The Aspergillus branching response is specific to a subset of oxylipins and is signaled through G-protein coupled receptors. RNA-Seq profiling shows differential expression of many transcription factors in response to 5,8-diHODE. Screening of null mutants of 33 of those transcription factors identifies three transcriptional regulators that appear to mediate the Aspergillus branching response; one of the mutants is locked in a hypo-branching phenotype, while the other two mutants display a hyper-branching phenotype. Our work reveals an endogenous signal that triggers crucial developmental processes in filamentous fungi, and opens new avenues for research on the morphogenesis of filamentous fungi.
published_date 2020-10-14T20:28:40Z
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