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Stroboscopic fluorescence lifetime imaging

Mark D Holton, Oscar R Silvestre, Rachel J Errington, Paul J Smith, Daniel R Matthews, Paul Rees Orcid Logo, Huw Summers Orcid Logo

Optics Express, Volume: 17, Issue: 7, Pages: 5205 - 5216

Swansea University Authors: Paul Rees Orcid Logo, Huw Summers Orcid Logo

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DOI (Published version): 10.1364/OE.17.005205

Published in: Optics Express
ISSN: 1094-4087
Published: 2009
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URI: https://cronfa.swan.ac.uk/Record/cronfa6048
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first_indexed 2013-07-23T11:56:02Z
last_indexed 2018-02-09T04:33:10Z
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spelling 2016-08-16T11:26:50.0961030 v2 6048 2013-09-03 Stroboscopic fluorescence lifetime imaging 537a2fe031a796a3bde99679ee8c24f5 0000-0002-7715-6914 Paul Rees Paul Rees true false a61c15e220837ebfa52648c143769427 0000-0002-0898-5612 Huw Summers Huw Summers true false 2013-09-03 MEDE Journal Article Optics Express 17 7 5205 5216 1094-4087 31 12 2009 2009-12-31 10.1364/OE.17.005205 This paper presents the first demonstration of a wholly novel experimental technique for time resolved fluorescence imaging. There are two established approaches to temporal resolution, i. direct observation in time domain using fast detectors or ii. indirect observation from frequency response measurements. This paper presents an approach based on modulated pulsed excitation (stroboscopic) and d.c. detection. It transforms the measurement of fluorescence lifetime in that it uses time integrated detection thus avoiding the usual trade-off between high speed resolution and low detection signal. The work was funded by EPSRC grant, EP/E013104/1 COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2016-08-16T11:26:50.0961030 2013-09-03T06:35:25.0000000 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Mark D Holton 1 Oscar R Silvestre 2 Rachel J Errington 3 Paul J Smith 4 Daniel R Matthews 5 Paul Rees 0000-0002-7715-6914 6 Huw Summers 0000-0002-0898-5612 7
title Stroboscopic fluorescence lifetime imaging
spellingShingle Stroboscopic fluorescence lifetime imaging
Paul Rees
Huw Summers
title_short Stroboscopic fluorescence lifetime imaging
title_full Stroboscopic fluorescence lifetime imaging
title_fullStr Stroboscopic fluorescence lifetime imaging
title_full_unstemmed Stroboscopic fluorescence lifetime imaging
title_sort Stroboscopic fluorescence lifetime imaging
author_id_str_mv 537a2fe031a796a3bde99679ee8c24f5
a61c15e220837ebfa52648c143769427
author_id_fullname_str_mv 537a2fe031a796a3bde99679ee8c24f5_***_Paul Rees
a61c15e220837ebfa52648c143769427_***_Huw Summers
author Paul Rees
Huw Summers
author2 Mark D Holton
Oscar R Silvestre
Rachel J Errington
Paul J Smith
Daniel R Matthews
Paul Rees
Huw Summers
format Journal article
container_title Optics Express
container_volume 17
container_issue 7
container_start_page 5205
publishDate 2009
institution Swansea University
issn 1094-4087
doi_str_mv 10.1364/OE.17.005205
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
document_store_str 0
active_str 0
published_date 2009-12-31T03:07:25Z
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score 11.013148

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