Stroboscopic fluorescence lifetime imaging

Journal article 1089 views

Mark D Holton, Oscar R Silvestre, Rachel J Errington, Paul J Smith, Daniel R Matthews, Paul Rees

, Huw Summers

Optics Express, Volume: 17, Issue: 7, Pages: 5205 - 5216

Swansea University Authors: Paul Rees , Huw Summers

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DOI (Published version): 10.1364/OE.17.005205

Published in:	Optics Express
ISSN:	1094-4087
Published:	2009
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa6048

Item Description:	This paper presents the first demonstration of a wholly novel experimental technique for time resolved fluorescence imaging. There are two established approaches to temporal resolution, i. direct observation in time domain using fast detectors or ii. indirect observation from frequency response measurements. This paper presents an approach based on modulated pulsed excitation (stroboscopic) and d.c. detection. It transforms the measurement of fluorescence lifetime in that it uses time integrated detection thus avoiding the usual trade-off between high speed resolution and low detection signal. The work was funded by EPSRC grant, EP/E013104/1
College:	Faculty of Science and Engineering
Issue:	7
Start Page:	5205
End Page:	5216