Journal article 1374 views 168 downloads
Cholesterolomics: An update
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Jonas Abdel-Khalik,
Eylan Yutuc ,
Alwena Morgan ,
Ian Gilmore,
Thomas Hearn,
Yuqin Wang
Analytical Biochemistry
Swansea University Authors:
William Griffiths , Eylan Yutuc , Alwena Morgan
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Open Access funded by Biotechnology and Biological Sciences Research Council. Published under a Creative Commons CC-BY 4.0 License.
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DOI (Published version): 10.1016/j.ab.2017.01.009
Abstract
Cholesterolomics can be regarded as the identification and quantification of cholesterol, its precursors post squalene, and metabolites of cholesterol and of its precursors, in a biological sample. These molecules include 1,25-dihydroxyvitamin D3, steroid hormones and bile acids and intermediates in...
| Published in: | Analytical Biochemistry |
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| ISSN: | 00032697 |
| Published: |
2017
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa31729 |
| Abstract: |
Cholesterolomics can be regarded as the identification and quantification of cholesterol, its precursors post squalene, and metabolites of cholesterol and of its precursors, in a biological sample. These molecules include 1,25-dihydroxyvitamin D3, steroid hormones and bile acids and intermediates in their respective biosynthetic pathways. In this short article we will concentrate our attention on intermediates in bile acid biosynthesis pathways, in particular oxysterols and cholestenoic acids. These molecular classes are implicated in the aetiology of a diverse array of diseases including autoimmune disease, Parkinson's disease, motor neuron disease, breast cancer, the lysosomal storage disease Niemann-Pick type C and the autosomal recessive disorder Smith-Lemli-Opitz syndrome. Mass spectrometry (MS) is the dominant technology for sterol analysis including both gas-chromatography (GC)-MS and liquid chromatography (LC)-MS and more recently matrix-assisted laser desorption/ionisation (MALDI)-MS for tissue imaging studies. Here we will discuss exciting biological findings and recent analytical improvements. |
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| Keywords: |
Cholesterol; Oxysterols; Cholestenoic acids; Derivatisation; Mass spectrometry |
| College: |
Faculty of Medicine, Health and Life Sciences |