Journal article 1374 views 168 downloads
Cholesterolomics: An update
Analytical Biochemistry
Swansea University Authors:
William Griffiths , Eylan Yutuc
, Alwena Morgan
-
PDF | Version of Record
Open Access funded by Biotechnology and Biological Sciences Research Council. Published under a Creative Commons CC-BY 4.0 License.
Download (876.62KB)
DOI (Published version): 10.1016/j.ab.2017.01.009
Abstract
Cholesterolomics can be regarded as the identification and quantification of cholesterol, its precursors post squalene, and metabolites of cholesterol and of its precursors, in a biological sample. These molecules include 1,25-dihydroxyvitamin D3, steroid hormones and bile acids and intermediates in...
Published in: | Analytical Biochemistry |
---|---|
ISSN: | 00032697 |
Published: |
2017
|
Online Access: |
Check full text
|
URI: | https://cronfa.swan.ac.uk/Record/cronfa31729 |
first_indexed |
2017-01-24T14:48:47Z |
---|---|
last_indexed |
2018-02-09T05:18:53Z |
id |
cronfa31729 |
recordtype |
SURis |
fullrecord |
<?xml version="1.0"?><rfc1807><datestamp>2018-01-11T10:30:15.4514295</datestamp><bib-version>v2</bib-version><id>31729</id><entry>2017-01-24</entry><title>Cholesterolomics: An update</title><swanseaauthors><author><sid>3316b1d1b524be1831790933eed1c26e</sid><ORCID>0000-0002-4129-6616</ORCID><firstname>William</firstname><surname>Griffiths</surname><name>William Griffiths</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>99332f073ce913a9b7d8b6441b17516d</sid><ORCID>0000-0001-9971-1950</ORCID><firstname>Eylan</firstname><surname>Yutuc</surname><name>Eylan Yutuc</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>9ea39c3d0935c897cb9fcd3ba550af71</sid><ORCID>0000-0002-3441-5357</ORCID><firstname>Alwena</firstname><surname>Morgan</surname><name>Alwena Morgan</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2017-01-24</date><deptcode>MEDS</deptcode><abstract>Cholesterolomics can be regarded as the identification and quantification of cholesterol, its precursors post squalene, and metabolites of cholesterol and of its precursors, in a biological sample. These molecules include 1,25-dihydroxyvitamin D3, steroid hormones and bile acids and intermediates in their respective biosynthetic pathways. In this short article we will concentrate our attention on intermediates in bile acid biosynthesis pathways, in particular oxysterols and cholestenoic acids. These molecular classes are implicated in the aetiology of a diverse array of diseases including autoimmune disease, Parkinson's disease, motor neuron disease, breast cancer, the lysosomal storage disease Niemann-Pick type C and the autosomal recessive disorder Smith-Lemli-Opitz syndrome. Mass spectrometry (MS) is the dominant technology for sterol analysis including both gas-chromatography (GC)-MS and liquid chromatography (LC)-MS and more recently matrix-assisted laser desorption/ionisation (MALDI)-MS for tissue imaging studies. Here we will discuss exciting biological findings and recent analytical improvements.</abstract><type>Journal Article</type><journal>Analytical Biochemistry</journal><publisher/><issnPrint>00032697</issnPrint><keywords>Cholesterol; Oxysterols; Cholestenoic acids; Derivatisation; Mass spectrometry</keywords><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2017</publishedYear><publishedDate>2017-12-31</publishedDate><doi>10.1016/j.ab.2017.01.009</doi><url/><notes/><college>COLLEGE NANME</college><department>Medical School</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>MEDS</DepartmentCode><institution>Swansea University</institution><degreesponsorsfunders>RCUK, BB/I001735/1</degreesponsorsfunders><apcterm/><lastEdited>2018-01-11T10:30:15.4514295</lastEdited><Created>2017-01-24T09:54:20.2325776</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>William</firstname><surname>Griffiths</surname><orcid>0000-0002-4129-6616</orcid><order>1</order></author><author><firstname>Jonas</firstname><surname>Abdel-Khalik</surname><order>2</order></author><author><firstname>Eylan</firstname><surname>Yutuc</surname><orcid>0000-0001-9971-1950</orcid><order>3</order></author><author><firstname>Alwena</firstname><surname>Morgan</surname><orcid>0000-0002-3441-5357</orcid><order>4</order></author><author><firstname>Ian</firstname><surname>Gilmore</surname><order>5</order></author><author><firstname>Thomas</firstname><surname>Hearn</surname><order>6</order></author><author><firstname>Yuqin</firstname><surname>Wang</surname><order>7</order></author></authors><documents><document><filename>0031729-11012018102853.pdf</filename><originalFilename>APCCD29.1-s2.0-S0003269717300209-main.pdf</originalFilename><uploaded>2018-01-11T10:28:53.7870000</uploaded><type>Output</type><contentLength>890304</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><embargoDate>2018-01-11T00:00:00.0000000</embargoDate><documentNotes>Open Access funded by Biotechnology and Biological Sciences Research Council. Published under a Creative Commons CC-BY 4.0 License.</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807> |
spelling |
2018-01-11T10:30:15.4514295 v2 31729 2017-01-24 Cholesterolomics: An update 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 99332f073ce913a9b7d8b6441b17516d 0000-0001-9971-1950 Eylan Yutuc Eylan Yutuc true false 9ea39c3d0935c897cb9fcd3ba550af71 0000-0002-3441-5357 Alwena Morgan Alwena Morgan true false 2017-01-24 MEDS Cholesterolomics can be regarded as the identification and quantification of cholesterol, its precursors post squalene, and metabolites of cholesterol and of its precursors, in a biological sample. These molecules include 1,25-dihydroxyvitamin D3, steroid hormones and bile acids and intermediates in their respective biosynthetic pathways. In this short article we will concentrate our attention on intermediates in bile acid biosynthesis pathways, in particular oxysterols and cholestenoic acids. These molecular classes are implicated in the aetiology of a diverse array of diseases including autoimmune disease, Parkinson's disease, motor neuron disease, breast cancer, the lysosomal storage disease Niemann-Pick type C and the autosomal recessive disorder Smith-Lemli-Opitz syndrome. Mass spectrometry (MS) is the dominant technology for sterol analysis including both gas-chromatography (GC)-MS and liquid chromatography (LC)-MS and more recently matrix-assisted laser desorption/ionisation (MALDI)-MS for tissue imaging studies. Here we will discuss exciting biological findings and recent analytical improvements. Journal Article Analytical Biochemistry 00032697 Cholesterol; Oxysterols; Cholestenoic acids; Derivatisation; Mass spectrometry 31 12 2017 2017-12-31 10.1016/j.ab.2017.01.009 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University RCUK, BB/I001735/1 2018-01-11T10:30:15.4514295 2017-01-24T09:54:20.2325776 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine William Griffiths 0000-0002-4129-6616 1 Jonas Abdel-Khalik 2 Eylan Yutuc 0000-0001-9971-1950 3 Alwena Morgan 0000-0002-3441-5357 4 Ian Gilmore 5 Thomas Hearn 6 Yuqin Wang 7 0031729-11012018102853.pdf APCCD29.1-s2.0-S0003269717300209-main.pdf 2018-01-11T10:28:53.7870000 Output 890304 application/pdf Version of Record true 2018-01-11T00:00:00.0000000 Open Access funded by Biotechnology and Biological Sciences Research Council. Published under a Creative Commons CC-BY 4.0 License. true eng |
title |
Cholesterolomics: An update |
spellingShingle |
Cholesterolomics: An update William Griffiths Eylan Yutuc Alwena Morgan |
title_short |
Cholesterolomics: An update |
title_full |
Cholesterolomics: An update |
title_fullStr |
Cholesterolomics: An update |
title_full_unstemmed |
Cholesterolomics: An update |
title_sort |
Cholesterolomics: An update |
author_id_str_mv |
3316b1d1b524be1831790933eed1c26e 99332f073ce913a9b7d8b6441b17516d 9ea39c3d0935c897cb9fcd3ba550af71 |
author_id_fullname_str_mv |
3316b1d1b524be1831790933eed1c26e_***_William Griffiths 99332f073ce913a9b7d8b6441b17516d_***_Eylan Yutuc 9ea39c3d0935c897cb9fcd3ba550af71_***_Alwena Morgan |
author |
William Griffiths Eylan Yutuc Alwena Morgan |
author2 |
William Griffiths Jonas Abdel-Khalik Eylan Yutuc Alwena Morgan Ian Gilmore Thomas Hearn Yuqin Wang |
format |
Journal article |
container_title |
Analytical Biochemistry |
publishDate |
2017 |
institution |
Swansea University |
issn |
00032697 |
doi_str_mv |
10.1016/j.ab.2017.01.009 |
college_str |
Faculty of Medicine, Health and Life Sciences |
hierarchytype |
|
hierarchy_top_id |
facultyofmedicinehealthandlifesciences |
hierarchy_top_title |
Faculty of Medicine, Health and Life Sciences |
hierarchy_parent_id |
facultyofmedicinehealthandlifesciences |
hierarchy_parent_title |
Faculty of Medicine, Health and Life Sciences |
department_str |
Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
document_store_str |
1 |
active_str |
0 |
description |
Cholesterolomics can be regarded as the identification and quantification of cholesterol, its precursors post squalene, and metabolites of cholesterol and of its precursors, in a biological sample. These molecules include 1,25-dihydroxyvitamin D3, steroid hormones and bile acids and intermediates in their respective biosynthetic pathways. In this short article we will concentrate our attention on intermediates in bile acid biosynthesis pathways, in particular oxysterols and cholestenoic acids. These molecular classes are implicated in the aetiology of a diverse array of diseases including autoimmune disease, Parkinson's disease, motor neuron disease, breast cancer, the lysosomal storage disease Niemann-Pick type C and the autosomal recessive disorder Smith-Lemli-Opitz syndrome. Mass spectrometry (MS) is the dominant technology for sterol analysis including both gas-chromatography (GC)-MS and liquid chromatography (LC)-MS and more recently matrix-assisted laser desorption/ionisation (MALDI)-MS for tissue imaging studies. Here we will discuss exciting biological findings and recent analytical improvements. |
published_date |
2017-12-31T06:56:57Z |
_version_ |
1826642268830826496 |
score |
11.054383 |