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Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides

Alejandra Culebro, Joana Revez, Ben Pascoe, Yasmin Friedmann, Matthew D. Hitchings, Jacek Stupak, Samuel K. Sheppard, Jianjun Li, Mirko Rossi, Ben Pascoe Orcid Logo, Yasmin Friedmann, Matthew Hitchings Orcid Logo

Journal of Bacteriology, Volume: 198, Issue: 20, Pages: 2829 - 2840

Swansea University Authors: Ben Pascoe Orcid Logo, Yasmin Friedmann, Matthew Hitchings Orcid Logo

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DOI (Published version): 10.1128/jb.00347-16

Abstract

Despite the importance of lipooligosaccharides (LOS) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in C. coli. In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates s...

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Published in: Journal of Bacteriology
ISSN: 0021-9193 1098-5530
Published: American Society for Microbiology 2016
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URI: https://cronfa.swan.ac.uk/Record/cronfa27686
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In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species. Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to identify mechanisms consistent with the generation of LOS phenotypic heterogeneity. After classifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought. This genetic diversity is at the basis of a completely unexplored LOS structure heterogeneity. Mass spectrometry analysis of the LOS of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from C. jejuni's. GlcN-GlcN disaccharides were present in the lipid A backbone in contrast to the GlcN3N-GlcN backbone observed in C. jejuni. Moreover, despite that many of the genes putatively involved in Qui3pNAcyl were absence in the genomes of various isolates, this rare sugar was found in the outer core of all C. coli. Therefore, regardless the high genetic diversity of LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS which might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.</abstract><type>Journal Article</type><journal>Journal of Bacteriology</journal><volume>198</volume><journalNumber>20</journalNumber><paginationStart>2829</paginationStart><paginationEnd>2840</paginationEnd><publisher>American Society for Microbiology</publisher><issnPrint>0021-9193</issnPrint><issnElectronic>1098-5530</issnElectronic><keywords/><publishedDay>15</publishedDay><publishedMonth>10</publishedMonth><publishedYear>2016</publishedYear><publishedDate>2016-10-15</publishedDate><doi>10.1128/jb.00347-16</doi><url/><notes/><college>COLLEGE NANME</college><department>Medicine</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>PMSC</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2020-06-25T16:57:22.2554463</lastEdited><Created>2016-05-04T15:14:38.9336467</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Alejandra</firstname><surname>Culebro</surname><order>1</order></author><author><firstname>Joana</firstname><surname>Revez</surname><order>2</order></author><author><firstname>Ben</firstname><surname>Pascoe</surname><order>3</order></author><author><firstname>Yasmin</firstname><surname>Friedmann</surname><order>4</order></author><author><firstname>Matthew D.</firstname><surname>Hitchings</surname><order>5</order></author><author><firstname>Jacek</firstname><surname>Stupak</surname><order>6</order></author><author><firstname>Samuel K.</firstname><surname>Sheppard</surname><order>7</order></author><author><firstname>Jianjun</firstname><surname>Li</surname><order>8</order></author><author><firstname>Mirko</firstname><surname>Rossi</surname><order>9</order></author><author><firstname>Ben</firstname><surname>Pascoe</surname><orcid>0000-0001-6376-5121</orcid><order>10</order></author><author><firstname>Yasmin</firstname><surname>Friedmann</surname><order>11</order></author><author><firstname>Matthew</firstname><surname>Hitchings</surname><orcid>0000-0002-5527-4709</orcid><order>12</order></author></authors><documents><document><filename>27686__8366__abdc22697ba54453a874659d65934ef6.pdf</filename><originalFilename>27686.pdf</originalFilename><uploaded>2018-03-22T13:59:33.7430000</uploaded><type>Output</type><contentLength>1015966</contentLength><contentType>application/pdf</contentType><version>Accepted Manuscript</version><cronfaStatus>true</cronfaStatus><embargoDate>2016-09-28T00:00:00.0000000</embargoDate><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling 2020-06-25T16:57:22.2554463 v2 27686 2016-05-04 Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides 4660c0eb7e6bfd796cd749ae713ea558 0000-0001-6376-5121 Ben Pascoe Ben Pascoe true false 94dd4462b95e78880040791727786dfc Yasmin Friedmann Yasmin Friedmann true false be98847c72c14a731c4a6b7bc02b3bcf 0000-0002-5527-4709 Matthew Hitchings Matthew Hitchings true false 2016-05-04 PMSC Despite the importance of lipooligosaccharides (LOS) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in C. coli. In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species. Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to identify mechanisms consistent with the generation of LOS phenotypic heterogeneity. After classifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought. This genetic diversity is at the basis of a completely unexplored LOS structure heterogeneity. Mass spectrometry analysis of the LOS of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from C. jejuni's. GlcN-GlcN disaccharides were present in the lipid A backbone in contrast to the GlcN3N-GlcN backbone observed in C. jejuni. Moreover, despite that many of the genes putatively involved in Qui3pNAcyl were absence in the genomes of various isolates, this rare sugar was found in the outer core of all C. coli. Therefore, regardless the high genetic diversity of LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS which might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation. Journal Article Journal of Bacteriology 198 20 2829 2840 American Society for Microbiology 0021-9193 1098-5530 15 10 2016 2016-10-15 10.1128/jb.00347-16 COLLEGE NANME Medicine COLLEGE CODE PMSC Swansea University 2020-06-25T16:57:22.2554463 2016-05-04T15:14:38.9336467 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Alejandra Culebro 1 Joana Revez 2 Ben Pascoe 3 Yasmin Friedmann 4 Matthew D. Hitchings 5 Jacek Stupak 6 Samuel K. Sheppard 7 Jianjun Li 8 Mirko Rossi 9 Ben Pascoe 0000-0001-6376-5121 10 Yasmin Friedmann 11 Matthew Hitchings 0000-0002-5527-4709 12 27686__8366__abdc22697ba54453a874659d65934ef6.pdf 27686.pdf 2018-03-22T13:59:33.7430000 Output 1015966 application/pdf Accepted Manuscript true 2016-09-28T00:00:00.0000000 true eng
title Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides
spellingShingle Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides
Ben Pascoe
Yasmin Friedmann
Matthew Hitchings
title_short Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides
title_full Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides
title_fullStr Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides
title_full_unstemmed Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides
title_sort Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides
author_id_str_mv 4660c0eb7e6bfd796cd749ae713ea558
94dd4462b95e78880040791727786dfc
be98847c72c14a731c4a6b7bc02b3bcf
author_id_fullname_str_mv 4660c0eb7e6bfd796cd749ae713ea558_***_Ben Pascoe
94dd4462b95e78880040791727786dfc_***_Yasmin Friedmann
be98847c72c14a731c4a6b7bc02b3bcf_***_Matthew Hitchings
author Ben Pascoe
Yasmin Friedmann
Matthew Hitchings
author2 Alejandra Culebro
Joana Revez
Ben Pascoe
Yasmin Friedmann
Matthew D. Hitchings
Jacek Stupak
Samuel K. Sheppard
Jianjun Li
Mirko Rossi
Ben Pascoe
Yasmin Friedmann
Matthew Hitchings
format Journal article
container_title Journal of Bacteriology
container_volume 198
container_issue 20
container_start_page 2829
publishDate 2016
institution Swansea University
issn 0021-9193
1098-5530
doi_str_mv 10.1128/jb.00347-16
publisher American Society for Microbiology
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description Despite the importance of lipooligosaccharides (LOS) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in C. coli. In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species. Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to identify mechanisms consistent with the generation of LOS phenotypic heterogeneity. After classifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought. This genetic diversity is at the basis of a completely unexplored LOS structure heterogeneity. Mass spectrometry analysis of the LOS of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from C. jejuni's. GlcN-GlcN disaccharides were present in the lipid A backbone in contrast to the GlcN3N-GlcN backbone observed in C. jejuni. Moreover, despite that many of the genes putatively involved in Qui3pNAcyl were absence in the genomes of various isolates, this rare sugar was found in the outer core of all C. coli. Therefore, regardless the high genetic diversity of LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS which might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
published_date 2016-10-15T03:33:38Z
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