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Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides

Alejandra Culebro, Joana Revez, Ben Pascoe, Yasmin Friedmann, Matthew D. Hitchings, Jacek Stupak, Samuel K. Sheppard, Jianjun Li, Mirko Rossi, Ben Pascoe Orcid Logo, Yasmin Friedmann, Matthew Hitchings Orcid Logo

Journal of Bacteriology, Volume: 198, Issue: 20, Pages: 2829 - 2840

Swansea University Authors: Ben Pascoe Orcid Logo, Yasmin Friedmann, Matthew Hitchings Orcid Logo

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DOI (Published version): 10.1128/jb.00347-16

Abstract

Despite the importance of lipooligosaccharides (LOS) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in C. coli. In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates s...

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Published in: Journal of Bacteriology
ISSN: 0021-9193 1098-5530
Published: American Society for Microbiology 2016
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URI: https://cronfa.swan.ac.uk/Record/cronfa27686
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Abstract: Despite the importance of lipooligosaccharides (LOS) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in C. coli. In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species. Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to identify mechanisms consistent with the generation of LOS phenotypic heterogeneity. After classifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought. This genetic diversity is at the basis of a completely unexplored LOS structure heterogeneity. Mass spectrometry analysis of the LOS of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from C. jejuni's. GlcN-GlcN disaccharides were present in the lipid A backbone in contrast to the GlcN3N-GlcN backbone observed in C. jejuni. Moreover, despite that many of the genes putatively involved in Qui3pNAcyl were absence in the genomes of various isolates, this rare sugar was found in the outer core of all C. coli. Therefore, regardless the high genetic diversity of LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS which might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.
College: Faculty of Medicine, Health and Life Sciences
Issue: 20
Start Page: 2829
End Page: 2840

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