Cholesterol metabolites exported from human brain

Iuliano, Luigi; Crick, Peter J.; Zerbinati, Chiara; Tritapepe, Luigi; Abdel-Khalik, Jonas; Poirot, Marc; Wang, Yuqin; Griffiths, William

doi:10.1016/j.steroids.2015.01.026

Journal article 1260 views

Cholesterol metabolites exported from human brain

Luigi Iuliano, Peter J. Crick, Chiara Zerbinati, Luigi Tritapepe, Jonas Abdel-Khalik, Marc Poirot, Yuqin Wang

, William Griffiths

Steroids

Swansea University Authors: Yuqin Wang , William Griffiths

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DOI (Published version): 10.1016/j.steroids.2015.01.026

Abstract

The human brain contains approximately 25% of the body's cholesterol. The brain is separated from the circulation by the blood brain barrier. While cholesterol will not passes this barrier, oxygenated forms of cholesterol can cross the barrier. Here by measuring the difference in the oxysterol...

Full description

Published in:	Steroids
Published:	2015
URI:	https://cronfa.swan.ac.uk/Record/cronfa20171

first_indexed	2015-02-13T02:57:56Z
last_indexed	2018-02-09T04:56:24Z
id	cronfa20171
recordtype	SURis
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spelling	2015-03-10T09:51:18.5335452 v2 20171 2015-02-12 Cholesterol metabolites exported from human brain c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2015-02-12 MEDS The human brain contains approximately 25% of the body's cholesterol. The brain is separated from the circulation by the blood brain barrier. While cholesterol will not passes this barrier, oxygenated forms of cholesterol can cross the barrier. Here by measuring the difference in the oxysterol content of blood plasma in the jugular vein and in a forearm vein by mass spectrometry (MS) we were able to determine the flux of more than 20 cholesterol metabolites between brain and the circulation. We confirm that 24S-hydroxycholesterol is exported from brain at a rate of about 2 - 3 mg/24 hr. Gas chromatography (GC) - MS data shows that the cholesterol metabolites 5α-hydroxy-6-oxocholesterol (3β,5α-dihydroxycholestan-6-one), 7β-hydroxycholesterol and 7-oxocholesterol, generally considered to be formed through reactive oxygen species, are similarly exported from brain at rates of about 0.1, 2 and 2 mg/24 hr, respectively. Although not to statistical significance both GC-MS and liquid chromatography (LC) - MS methods indicate that (25R)26-hydroxycholesterol is imported to brain, while LC-MS indicates that 7α-hydroxy-3-oxocholest-4-enoic acid is exported from brain. Journal Article Steroids 31 12 2015 2015-12-31 10.1016/j.steroids.2015.01.026 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2015-03-10T09:51:18.5335452 2015-02-12T12:50:51.7696862 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Luigi Iuliano 1 Peter J. Crick 2 Chiara Zerbinati 3 Luigi Tritapepe 4 Jonas Abdel-Khalik 5 Marc Poirot 6 Yuqin Wang 0000-0002-3063-3066 7 William Griffiths 0000-0002-4129-6616 8
title	Cholesterol metabolites exported from human brain
spellingShingle	Cholesterol metabolites exported from human brain Yuqin Wang William Griffiths
title_short	Cholesterol metabolites exported from human brain
title_full	Cholesterol metabolites exported from human brain
title_fullStr	Cholesterol metabolites exported from human brain
title_full_unstemmed	Cholesterol metabolites exported from human brain
title_sort	Cholesterol metabolites exported from human brain
author_id_str_mv	c92729b58622f9fdf6a0e7d8f4ce5081 3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv	c92729b58622f9fdf6a0e7d8f4ce5081_*_Yuqin Wang 3316b1d1b524be1831790933eed1c26e_*_William Griffiths
author	Yuqin Wang William Griffiths
author2	Luigi Iuliano Peter J. Crick Chiara Zerbinati Luigi Tritapepe Jonas Abdel-Khalik Marc Poirot Yuqin Wang William Griffiths
format	Journal article
container_title	Steroids
publishDate	2015
institution	Swansea University
doi_str_mv	10.1016/j.steroids.2015.01.026
college_str	Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id	facultyofmedicinehealthandlifesciences
hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str	0
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description	The human brain contains approximately 25% of the body's cholesterol. The brain is separated from the circulation by the blood brain barrier. While cholesterol will not passes this barrier, oxygenated forms of cholesterol can cross the barrier. Here by measuring the difference in the oxysterol content of blood plasma in the jugular vein and in a forearm vein by mass spectrometry (MS) we were able to determine the flux of more than 20 cholesterol metabolites between brain and the circulation. We confirm that 24S-hydroxycholesterol is exported from brain at a rate of about 2 - 3 mg/24 hr. Gas chromatography (GC) - MS data shows that the cholesterol metabolites 5α-hydroxy-6-oxocholesterol (3β,5α-dihydroxycholestan-6-one), 7β-hydroxycholesterol and 7-oxocholesterol, generally considered to be formed through reactive oxygen species, are similarly exported from brain at rates of about 0.1, 2 and 2 mg/24 hr, respectively. Although not to statistical significance both GC-MS and liquid chromatography (LC) - MS methods indicate that (25R)26-hydroxycholesterol is imported to brain, while LC-MS indicates that 7α-hydroxy-3-oxocholest-4-enoic acid is exported from brain.
published_date	2015-12-31T00:45:39Z
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score	11.048453

Cholesterol metabolites exported from human brain

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