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Generic epitaxial graphene biosensors for ultrasensitive detection of cancer risk biomarker
Zari Tehrani 
,
G Burwell,
M A Mohd Azmi,
A Castaing,
R Rickman,
J Almarashi,
Peter Dunstan ,
A Miran Beigi,
Shareen Doak ,
Owen Guy
,
G Burwell,
M A Mohd Azmi,
A Castaing,
R Rickman,
J Almarashi,
Peter Dunstan ,
A Miran Beigi,
Shareen Doak ,
Owen Guy
2D Materials, Volume: 1, Issue: 2, Start page: 025004
Swansea University Authors:
Zari Tehrani , Peter Dunstan , Shareen Doak , Owen Guy
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DOI (Published version): 10.1088/2053-1583/1/2/025004
Abstract
A generic electrochemical method of 'bioreceptor' antibody attachment to phenyl amine functionalized graphitic surfaces is demonstrated. Micro-channels of chemically modified multi-layer epitaxial graphene (MLEG) have been used to provide a repeatable and reliable response to nano-molar (n...
| Published in: | 2D Materials |
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| ISSN: | 2053-1583 |
| Published: |
2014
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa19735 |
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| Abstract: |
A generic electrochemical method of 'bioreceptor' antibody attachment to phenyl amine functionalized graphitic surfaces is demonstrated. Micro-channels of chemically modified multi-layer epitaxial graphene (MLEG) have been used to provide a repeatable and reliable response to nano-molar (nM) concentrations of the cancer risk (oxidative stress) biomarker 8-hydroxydeoxyguanosine (8-OHdG). X-ray photoelectron spectroscopy, Raman spectroscopy are used to characterize the functionalized MLEG. Confocal fluorescence microscopy using fluorescent-labelled antibodies indicates that the anti-8-OHdG antibody selectively binds to the phenyl amine-functionalized MLEG's channel. Current–voltage measurements on functionalized channels showed repeatable current responses from antibody–biomarker binding events. This technique is scalable, reliable, and capable of providing a rapid, quantitative, label-free assessment of biomarkers at nano-molar (<20 nM) concentrations in analyte solutions. The sensitivity of the sensor device was investigated using varying concentrations of 8-OHdG, with changes in the sensor's channel resistance observed upon exposure to 8-OHdG. Detection of 8-OHdG concentrations as low as 0.1 ng ml−1 (0.35 nM) has been demonstrated. This is five times more sensitive than reported enzyme linked immunosorbent assay tests (0.5 ng ml−1). |
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| Issue: |
2 |
| Start Page: |
025004 |