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Children with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/-) regulatory T cell function.

M Smith, MR Tourigny, P Noakes, CA Thornton, MK Tulic, SL Prescott, Cathy Thornton Orcid Logo

Journal of Allergy and Clinical Immunology, Volume: 121, Issue: 6, Pages: 1460 - 1466

Swansea University Author: Cathy Thornton Orcid Logo

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DOI (Published version): 10.1016/j.jaci.2008.03.025

Abstract

BACKGROUND: The role of regulatory T (Treg) cells in allergic predisposition is not known.OBJECTIVE: This study compared the frequency and function of cord blood Treg cells from nonallergic children (n = 18) with those from children who have egg allergy (n = 15) in the first year of life.METHODS: CD...

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Published in: Journal of Allergy and Clinical Immunology
ISSN: 00916749
Published: Elsevier 2008
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URI: https://cronfa.swan.ac.uk/Record/cronfa9993
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fullrecord <?xml version="1.0"?><rfc1807><datestamp>2013-11-08T11:15:21.4242948</datestamp><bib-version>v2</bib-version><id>9993</id><entry>2012-03-21</entry><title>Children with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/-) regulatory T cell function.</title><swanseaauthors><author><sid>c71a7a4be7361094d046d312202bce0c</sid><ORCID>0000-0002-5153-573X</ORCID><firstname>Cathy</firstname><surname>Thornton</surname><name>Cathy Thornton</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2012-03-21</date><deptcode>BMS</deptcode><abstract>BACKGROUND: The role of regulatory T (Treg) cells in allergic predisposition is not known.OBJECTIVE: This study compared the frequency and function of cord blood Treg cells from nonallergic children (n = 18) with those from children who have egg allergy (n = 15) in the first year of life.METHODS: CD4(+) effector T cells and autologous antigen-presenting cells isolated from cord blood mononuclear cells were cocultured with or without CD4(+)CD25(+)CD127(lo/-) Treg cells, and cytokine responses to staphylococcal endotoxin B were assessed after 48 hours.RESULTS: The addition of Treg cell populations to cord blood mononuclear cell cultures resulted in significant reduction in IL-10 (P = .002), IL-13 (P = .012), and IFN-gamma (P &lt; .001) production. Consistent with other reports, effector CD4(+) T-cell responses (IFN-gamma and IL-13) tended to be lower in the allergic group. These neonates showed less significant Treg cell-associated suppression of IFN-gamma (P = .015) compared with that seen in the nonallergic group (P = .001). The allergic group was also less likely (44%) to show Treg cell-associated suppression of IFN-gamma effector responses compared with that seen in the nonallergic group (78%, P = .015). The magnitude of suppression (change in IFN-gamma level when CD4(+)CD25(+)CD127(lo/-) Treg cells were added to responding effector T-cell cultures) was significantly lower in the allergic group (P = .004). There were no between-group differences in the circulating CD4(+)CD25(+)CD127(lo/-) Treg cells (as a percentage of cord blood T cells) or in the FOXP3 expression of these cells.CONCLUSION: This study confirms the presence and activity of Treg cells in cord blood and provides preliminary evidence of differences in neonates who progress to allergic disease in the first year of life.</abstract><type>Journal Article</type><journal>Journal of Allergy and Clinical Immunology</journal><volume>121</volume><journalNumber>6</journalNumber><paginationStart>1460</paginationStart><paginationEnd>1466</paginationEnd><publisher>Elsevier</publisher><issnPrint>00916749</issnPrint><issnElectronic/><keywords>Regulatory T cells, cord blood, neonates, food allergy, allergic disease, egg allergy, cytokines, infants</keywords><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2008</publishedYear><publishedDate>2008-12-31</publishedDate><doi>10.1016/j.jaci.2008.03.025</doi><url>http://www.sciencedirect.com/science/article/pii/S009167490800609X</url><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2013-11-08T11:15:21.4242948</lastEdited><Created>2012-03-21T16:17:16.0000000</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>M</firstname><surname>Smith</surname><order>1</order></author><author><firstname>MR</firstname><surname>Tourigny</surname><order>2</order></author><author><firstname>P</firstname><surname>Noakes</surname><order>3</order></author><author><firstname>CA</firstname><surname>Thornton</surname><order>4</order></author><author><firstname>MK</firstname><surname>Tulic</surname><order>5</order></author><author><firstname>SL</firstname><surname>Prescott</surname><order>6</order></author><author><firstname>Cathy</firstname><surname>Thornton</surname><orcid>0000-0002-5153-573X</orcid><order>7</order></author></authors><documents/><OutputDurs/></rfc1807>
spelling 2013-11-08T11:15:21.4242948 v2 9993 2012-03-21 Children with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/-) regulatory T cell function. c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 2012-03-21 BMS BACKGROUND: The role of regulatory T (Treg) cells in allergic predisposition is not known.OBJECTIVE: This study compared the frequency and function of cord blood Treg cells from nonallergic children (n = 18) with those from children who have egg allergy (n = 15) in the first year of life.METHODS: CD4(+) effector T cells and autologous antigen-presenting cells isolated from cord blood mononuclear cells were cocultured with or without CD4(+)CD25(+)CD127(lo/-) Treg cells, and cytokine responses to staphylococcal endotoxin B were assessed after 48 hours.RESULTS: The addition of Treg cell populations to cord blood mononuclear cell cultures resulted in significant reduction in IL-10 (P = .002), IL-13 (P = .012), and IFN-gamma (P < .001) production. Consistent with other reports, effector CD4(+) T-cell responses (IFN-gamma and IL-13) tended to be lower in the allergic group. These neonates showed less significant Treg cell-associated suppression of IFN-gamma (P = .015) compared with that seen in the nonallergic group (P = .001). The allergic group was also less likely (44%) to show Treg cell-associated suppression of IFN-gamma effector responses compared with that seen in the nonallergic group (78%, P = .015). The magnitude of suppression (change in IFN-gamma level when CD4(+)CD25(+)CD127(lo/-) Treg cells were added to responding effector T-cell cultures) was significantly lower in the allergic group (P = .004). There were no between-group differences in the circulating CD4(+)CD25(+)CD127(lo/-) Treg cells (as a percentage of cord blood T cells) or in the FOXP3 expression of these cells.CONCLUSION: This study confirms the presence and activity of Treg cells in cord blood and provides preliminary evidence of differences in neonates who progress to allergic disease in the first year of life. Journal Article Journal of Allergy and Clinical Immunology 121 6 1460 1466 Elsevier 00916749 Regulatory T cells, cord blood, neonates, food allergy, allergic disease, egg allergy, cytokines, infants 31 12 2008 2008-12-31 10.1016/j.jaci.2008.03.025 http://www.sciencedirect.com/science/article/pii/S009167490800609X COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2013-11-08T11:15:21.4242948 2012-03-21T16:17:16.0000000 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine M Smith 1 MR Tourigny 2 P Noakes 3 CA Thornton 4 MK Tulic 5 SL Prescott 6 Cathy Thornton 0000-0002-5153-573X 7
title Children with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/-) regulatory T cell function.
spellingShingle Children with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/-) regulatory T cell function.
Cathy Thornton
title_short Children with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/-) regulatory T cell function.
title_full Children with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/-) regulatory T cell function.
title_fullStr Children with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/-) regulatory T cell function.
title_full_unstemmed Children with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/-) regulatory T cell function.
title_sort Children with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/-) regulatory T cell function.
author_id_str_mv c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton
author Cathy Thornton
author2 M Smith
MR Tourigny
P Noakes
CA Thornton
MK Tulic
SL Prescott
Cathy Thornton
format Journal article
container_title Journal of Allergy and Clinical Immunology
container_volume 121
container_issue 6
container_start_page 1460
publishDate 2008
institution Swansea University
issn 00916749
doi_str_mv 10.1016/j.jaci.2008.03.025
publisher Elsevier
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url http://www.sciencedirect.com/science/article/pii/S009167490800609X
document_store_str 0
active_str 0
description BACKGROUND: The role of regulatory T (Treg) cells in allergic predisposition is not known.OBJECTIVE: This study compared the frequency and function of cord blood Treg cells from nonallergic children (n = 18) with those from children who have egg allergy (n = 15) in the first year of life.METHODS: CD4(+) effector T cells and autologous antigen-presenting cells isolated from cord blood mononuclear cells were cocultured with or without CD4(+)CD25(+)CD127(lo/-) Treg cells, and cytokine responses to staphylococcal endotoxin B were assessed after 48 hours.RESULTS: The addition of Treg cell populations to cord blood mononuclear cell cultures resulted in significant reduction in IL-10 (P = .002), IL-13 (P = .012), and IFN-gamma (P < .001) production. Consistent with other reports, effector CD4(+) T-cell responses (IFN-gamma and IL-13) tended to be lower in the allergic group. These neonates showed less significant Treg cell-associated suppression of IFN-gamma (P = .015) compared with that seen in the nonallergic group (P = .001). The allergic group was also less likely (44%) to show Treg cell-associated suppression of IFN-gamma effector responses compared with that seen in the nonallergic group (78%, P = .015). The magnitude of suppression (change in IFN-gamma level when CD4(+)CD25(+)CD127(lo/-) Treg cells were added to responding effector T-cell cultures) was significantly lower in the allergic group (P = .004). There were no between-group differences in the circulating CD4(+)CD25(+)CD127(lo/-) Treg cells (as a percentage of cord blood T cells) or in the FOXP3 expression of these cells.CONCLUSION: This study confirms the presence and activity of Treg cells in cord blood and provides preliminary evidence of differences in neonates who progress to allergic disease in the first year of life.
published_date 2008-12-31T03:11:28Z
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