E-Thesis 286 views 215 downloads
An Electrochemical Biosensor for Sensitive in-situ Detection of Methyl-isoborneol (MIB) in Water / JOSHUA PEARCE
Swansea University Author: JOSHUA PEARCE
Abstract
2-Methylisoborneol (MIB) is an naturally occurring, odour producing compound which generates mass complaints due to its occurrence in drinking water. Removal and detection techniques are currently limited in the field due to the low detection level to the human nose being as low as 25ng/L. Current d...
| Published: |
Swansea University, Wales, UK
2025
|
|---|---|
| Institution: | Swansea University |
| Degree level: | Master of Research |
| Degree name: | MSc by Research |
| Supervisor: | Wei, Z. |
| URI: | https://cronfa.swan.ac.uk/Record/cronfa70398 |
| Abstract: |
2-Methylisoborneol (MIB) is an naturally occurring, odour producing compound which generates mass complaints due to its occurrence in drinking water. Removal and detection techniques are currently limited in the field due to the low detection level to the human nose being as low as 25ng/L. Current detection techniques consist of Gas Chromatography – Mass Spectrometry (GC/MS) which can struggle at detection levels such as this. This project will explore the potential of a sensing method which has both isolated selectivity to the target compound and has the ability for in-situ detection using an electrochemical biosensor, capable through the use of molecularly imprinted polymer (MIP) technology. The Brunner-Emmet-Teller (BET)nitrogen adsorption analysis, Raman spectroscopy and Scanning ElectronMicroscopy (SEM) were all used to characterize the sensor, while Cyclic voltammetry (CV) and Electrochemical impedance spectroscopy (EIS) were both used to analyse the electrochemical behaviour of the sensor. Ideal polymer conditions were found to be 10mL using a drop casting method, followed by a 48hour period in an oven at 40°C, with a sensor using a graphene, diacetone alcohol (DAA) and polydopamine (PDA) recipe was used on a Kapton polyimide tape. A template removal and reintroduction period of 25 minutes was found to be optimal, with the removal solution being a 1% ethanol mix with deionised water. Using the calibration curve from EIS testing, an LOD value of 1.45ng/L and LOQ value of 4.39ng/L were calculated. |
|---|---|
| Keywords: |
Electrochemical, Biosensor, MIB, Water, Detection |
| College: |
Faculty of Science and Engineering |
| Funders: |
Welsh Water, M2A |