The thrips gut pH and implications for symbiont-mediated RNAi

Andongma, Awawing Anjwengwo; (neeWhitten), Miranda Walker; Chofong, Gilbert N; Dyson, Paul

doi:10.1017/S0007485325000240

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The thrips gut pH and implications for symbiont-mediated RNAi

Awawing Anjwengwo Andongma, Miranda Walker (neeWhitten)

, Gilbert N Chofong, Paul Dyson

Bulletin of Entomological Research, Pages: 1 - 7

Swansea University Authors: Awawing Anjwengwo Andongma, Miranda Walker (neeWhitten) , Paul Dyson

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DOI (Published version): 10.1017/S0007485325000240

Abstract

The gut pH plays crucial roles in diet preference, habitat choice, insect fitness, and insect-microbial relationships. It significantly impacts enzyme activity efficiency, as well as the internalisation and efficacy of pesticides. Without a comprehensive understanding of the gut environment, potenti...

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Published in:	Bulletin of Entomological Research
ISSN:	0007-4853 1475-2670
Published:	Cambridge University Press (CUP) 2025
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa69453

first_indexed	2025-05-08T12:55:20Z
last_indexed	2025-05-09T07:04:51Z
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spelling	2025-05-08T13:56:55.1599073 v2 69453 2025-05-08 The thrips gut pH and implications for symbiont-mediated RNAi 6d83c753a85242a9fb9a366ab5636d69 Awawing Anjwengwo Andongma Awawing Anjwengwo Andongma true false 83389c57d000a704fae36fda87d7ca76 0000-0003-1930-5042 Miranda Walker (neeWhitten) Miranda Walker (neeWhitten) true false 300e3f46b70ae83f563b24f41d00cd17 0000-0002-0558-2666 Paul Dyson Paul Dyson true false 2025-05-08 MEDS The gut pH plays crucial roles in diet preference, habitat choice, insect fitness, and insect-microbial relationships. It significantly impacts enzyme activity efficiency, as well as the internalisation and efficacy of pesticides. Without a comprehensive understanding of the gut environment, potential pest management strategies cannot be fully optimised.This study investigates the gut pH of the globally invasive pest insect Western flower thrips , and the effect its Gram-negative symbiotic gut bacterium BFo2 has on pH modulation. Indicator dyes were fed to and the gut pH was found to vary between 6 and 7. In general, the larval and adult guts appear to have a pH of between 6 and 6.5; however, the posterior gut of some adults appears to be closer to 7. This almost neutral pH offers a favourable environment for the neutrophilic symbiotic BFo2. The ability of BFo2 isolates to buffer pH towards neutral was also observed during culture using broths at different pH values.This paper also discusses the implications of this gut environment on dsRNAi delivery. By laying the foundation for understanding how gut pH can be leveraged to enhance current pest management strategies, this study particularly benefits research aimed at optimising the delivery of lethal dsRNA through symbiont-mediated RNAi to Western flower thrips in pest management programs. Journal Article Bulletin of Entomological Research 0 1 7 Cambridge University Press (CUP) 0007-4853 1475-2670 BFo2, insect gut, pH indicator, symbiotic bacteria, thrips 16 4 2025 2025-04-16 10.1017/S0007485325000240 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University Another institution paid the OA fee This work was funded by TechAccel LLC and by a grant from UKRI (BBSRC grant ref. BB/R006148/1) to PD. 2025-05-08T13:56:55.1599073 2025-05-08T12:54:53.3642703 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Awawing Anjwengwo Andongma 1 Miranda Walker (neeWhitten) 0000-0003-1930-5042 2 Gilbert N Chofong 3 Paul Dyson 0000-0002-0558-2666 4 69453__34206__11e80710a69f4194aed6f6b6f801b052.pdf 69453.VOR.pdf 2025-05-08T13:53:21.4941679 Output 1469470 application/pdf Version of Record true © The Author(s), 2025. This is an Open Access article, distributed under the terms of the Creative Commons Attribution (CC BY) licence. true eng http://creativecommons.org/licenses/by/4.0
title	The thrips gut pH and implications for symbiont-mediated RNAi
spellingShingle	The thrips gut pH and implications for symbiont-mediated RNAi Awawing Anjwengwo Andongma Miranda Walker (neeWhitten) Paul Dyson
title_short	The thrips gut pH and implications for symbiont-mediated RNAi
title_full	The thrips gut pH and implications for symbiont-mediated RNAi
title_fullStr	The thrips gut pH and implications for symbiont-mediated RNAi
title_full_unstemmed	The thrips gut pH and implications for symbiont-mediated RNAi
title_sort	The thrips gut pH and implications for symbiont-mediated RNAi
author_id_str_mv	6d83c753a85242a9fb9a366ab5636d69 83389c57d000a704fae36fda87d7ca76 300e3f46b70ae83f563b24f41d00cd17
author_id_fullname_str_mv	6d83c753a85242a9fb9a366ab5636d69_*_Awawing Anjwengwo Andongma 83389c57d000a704fae36fda87d7ca76__Miranda Walker (neeWhitten) 300e3f46b70ae83f563b24f41d00cd17_**_Paul Dyson
author	Awawing Anjwengwo Andongma Miranda Walker (neeWhitten) Paul Dyson
author2	Awawing Anjwengwo Andongma Miranda Walker (neeWhitten) Gilbert N Chofong Paul Dyson
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description	The gut pH plays crucial roles in diet preference, habitat choice, insect fitness, and insect-microbial relationships. It significantly impacts enzyme activity efficiency, as well as the internalisation and efficacy of pesticides. Without a comprehensive understanding of the gut environment, potential pest management strategies cannot be fully optimised.This study investigates the gut pH of the globally invasive pest insect Western flower thrips , and the effect its Gram-negative symbiotic gut bacterium BFo2 has on pH modulation. Indicator dyes were fed to and the gut pH was found to vary between 6 and 7. In general, the larval and adult guts appear to have a pH of between 6 and 6.5; however, the posterior gut of some adults appears to be closer to 7. This almost neutral pH offers a favourable environment for the neutrophilic symbiotic BFo2. The ability of BFo2 isolates to buffer pH towards neutral was also observed during culture using broths at different pH values.This paper also discusses the implications of this gut environment on dsRNAi delivery. By laying the foundation for understanding how gut pH can be leveraged to enhance current pest management strategies, this study particularly benefits research aimed at optimising the delivery of lethal dsRNA through symbiont-mediated RNAi to Western flower thrips in pest management programs.
published_date	2025-04-16T05:47:25Z
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score	11.065032

The thrips gut pH and implications for symbiont-mediated RNAi

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