Journal article 26 views 7 downloads
The thrips gut pH and implications for symbiont-mediated RNAi
,
Gilbert N Chofong,
Paul Dyson
Bulletin of Entomological Research, Pages: 1 - 7
Swansea University Authors:
Awawing Anjwengwo Andongma, Miranda Walker (neeWhitten) , Paul Dyson
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© The Author(s), 2025. This is an Open Access article, distributed under the terms of the Creative Commons Attribution (CC BY) licence.
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DOI (Published version): 10.1017/S0007485325000240
Abstract
The gut pH plays crucial roles in diet preference, habitat choice, insect fitness, and insect-microbial relationships. It significantly impacts enzyme activity efficiency, as well as the internalisation and efficacy of pesticides. Without a comprehensive understanding of the gut environment, potenti...
| Published in: | Bulletin of Entomological Research |
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| ISSN: | 0007-4853 1475-2670 |
| Published: |
Cambridge University Press (CUP)
2025
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa69453 |
| Abstract: |
The gut pH plays crucial roles in diet preference, habitat choice, insect fitness, and insect-microbial relationships. It significantly impacts enzyme activity efficiency, as well as the internalisation and efficacy of pesticides. Without a comprehensive understanding of the gut environment, potential pest management strategies cannot be fully optimised.This study investigates the gut pH of the globally invasive pest insect Western flower thrips , and the effect its Gram-negative symbiotic gut bacterium BFo2 has on pH modulation. Indicator dyes were fed to and the gut pH was found to vary between 6 and 7. In general, the larval and adult guts appear to have a pH of between 6 and 6.5; however, the posterior gut of some adults appears to be closer to 7. This almost neutral pH offers a favourable environment for the neutrophilic symbiotic BFo2. The ability of BFo2 isolates to buffer pH towards neutral was also observed during culture using broths at different pH values.This paper also discusses the implications of this gut environment on dsRNAi delivery. By laying the foundation for understanding how gut pH can be leveraged to enhance current pest management strategies, this study particularly benefits research aimed at optimising the delivery of lethal dsRNA through symbiont-mediated RNAi to Western flower thrips in pest management programs. |
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| Keywords: |
BFo2, insect gut, pH indicator, symbiotic bacteria, thrips |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Funders: |
This work was funded by TechAccel LLC and by a grant from UKRI (BBSRC grant ref. BB/R006148/1) to PD. |
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