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Comparative genomics of Metarhizium brunneum strains V275 and ARSEF 4556: unraveling intraspecies diversity

Alexandra M Kortsinoglou Orcid Logo, Martyn J Wood, Antonis I Myridakis, Marios Andrikopoulos, Andreas Roussis Orcid Logo, Dan Eastwood Orcid Logo, Tariq Butt Orcid Logo, Vassili N Kouvelis Orcid Logo

G3: Genes, Genomes, Genetics, Volume: 14, Issue: 10, Start page: jkae190

Swansea University Authors: Dan Eastwood Orcid Logo, Tariq Butt Orcid Logo

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DOI (Published version): 10.1093/g3journal/jkae190

Abstract

Entomopathogenic fungi belonging to the Order Hypocreales are renowned for their ability to infect and kill insect hosts, while their endophytic mode of life and the beneficial rhizosphere effects on plant hosts have only been recently recognized. Understanding the molecular mechanisms underlying th...

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Published in: G3: Genes, Genomes, Genetics
ISSN: 2160-1836
Published: Oxford University Press (OUP) 2024
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URI: https://cronfa.swan.ac.uk/Record/cronfa68477
first_indexed 2024-12-06T13:48:58Z
last_indexed 2024-12-06T13:48:58Z
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Understanding the molecular mechanisms underlying their different lifestyles could optimize their potential as both biocontrol and biofertilizer agents, as well as the wider appreciation of niche plasticity in fungal ecology. This study describes the comprehensive whole genome sequencing and analysis of one of the most effective entomopathogenic and endophytic EPF strains, Metarhizium brunneum V275 (commercially known as Lalguard Met52), achieved through Nanopore and Illumina reads. Comparative genomics for exploring intraspecies variability and analyses of key gene sets were conducted with a second effective EPF strain, M. brunneum ARSEF 4556. The search for strain- or species-specific genes was extended to M. brunneum strain ARSEF 3297 and other species of genus Metarhizium, to identify molecular mechanisms and putative key genome adaptations associated with mode of life differences. Genome size differed significantly, with M. brunneum V275 having the largest genome amongst M. brunneum strains sequenced to date. Genome analyses revealed an abundance of plant-degrading enzymes, plant colonization-associated genes, and intriguing intraspecies variations regarding their predicted secondary metabolic compounds and the number and localization of Transposable Elements. 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spelling 2024-12-06T11:49:48.9154215 v2 68477 2024-12-06 Comparative genomics of Metarhizium brunneum strains V275 and ARSEF 4556: unraveling intraspecies diversity 4982f3fa83886c0362e2bb43ce1c027f 0000-0002-7015-0739 Dan Eastwood Dan Eastwood true false 85d1c2ddde272a1176e74978e25ebece 0000-0002-8789-9543 Tariq Butt Tariq Butt true false 2024-12-06 BGPS Entomopathogenic fungi belonging to the Order Hypocreales are renowned for their ability to infect and kill insect hosts, while their endophytic mode of life and the beneficial rhizosphere effects on plant hosts have only been recently recognized. Understanding the molecular mechanisms underlying their different lifestyles could optimize their potential as both biocontrol and biofertilizer agents, as well as the wider appreciation of niche plasticity in fungal ecology. This study describes the comprehensive whole genome sequencing and analysis of one of the most effective entomopathogenic and endophytic EPF strains, Metarhizium brunneum V275 (commercially known as Lalguard Met52), achieved through Nanopore and Illumina reads. Comparative genomics for exploring intraspecies variability and analyses of key gene sets were conducted with a second effective EPF strain, M. brunneum ARSEF 4556. The search for strain- or species-specific genes was extended to M. brunneum strain ARSEF 3297 and other species of genus Metarhizium, to identify molecular mechanisms and putative key genome adaptations associated with mode of life differences. Genome size differed significantly, with M. brunneum V275 having the largest genome amongst M. brunneum strains sequenced to date. Genome analyses revealed an abundance of plant-degrading enzymes, plant colonization-associated genes, and intriguing intraspecies variations regarding their predicted secondary metabolic compounds and the number and localization of Transposable Elements. The potential significance of the differences found between closely related endophytic and entomopathogenic fungi, regarding plant growth-promoting and entomopathogenic abilities, are discussed, enhancing our understanding of their diverse functionalities and putative applications in agriculture and ecology. Journal Article G3: Genes, Genomes, Genetics 14 10 jkae190 Oxford University Press (OUP) 2160-1836 Endophytic entomopathogenic fungi; Metarhizium brunneum; whole genome sequencing; biosynthetic gene clusters (BGCs); CAZymes; transposable elements (TEs) 31 10 2024 2024-10-31 10.1093/g3journal/jkae190 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University Another institution paid the OA fee The research work was supported by the Hellenic Foundation for Research and Innovation (HFRI) under the 3rd Call for HFRI PhD Fellowships (Fellowship Number: 5879). 2024-12-06T11:49:48.9154215 2024-12-06T11:42:55.3785424 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Alexandra M Kortsinoglou 0000-0002-4667-7290 1 Martyn J Wood 2 Antonis I Myridakis 3 Marios Andrikopoulos 4 Andreas Roussis 0000-0003-2346-7132 5 Dan Eastwood 0000-0002-7015-0739 6 Tariq Butt 0000-0002-8789-9543 7 Vassili N Kouvelis 0000-0001-6753-0872 8
title Comparative genomics of Metarhizium brunneum strains V275 and ARSEF 4556: unraveling intraspecies diversity
spellingShingle Comparative genomics of Metarhizium brunneum strains V275 and ARSEF 4556: unraveling intraspecies diversity
Dan Eastwood
Tariq Butt
title_short Comparative genomics of Metarhizium brunneum strains V275 and ARSEF 4556: unraveling intraspecies diversity
title_full Comparative genomics of Metarhizium brunneum strains V275 and ARSEF 4556: unraveling intraspecies diversity
title_fullStr Comparative genomics of Metarhizium brunneum strains V275 and ARSEF 4556: unraveling intraspecies diversity
title_full_unstemmed Comparative genomics of Metarhizium brunneum strains V275 and ARSEF 4556: unraveling intraspecies diversity
title_sort Comparative genomics of Metarhizium brunneum strains V275 and ARSEF 4556: unraveling intraspecies diversity
author_id_str_mv 4982f3fa83886c0362e2bb43ce1c027f
85d1c2ddde272a1176e74978e25ebece
author_id_fullname_str_mv 4982f3fa83886c0362e2bb43ce1c027f_***_Dan Eastwood
85d1c2ddde272a1176e74978e25ebece_***_Tariq Butt
author Dan Eastwood
Tariq Butt
author2 Alexandra M Kortsinoglou
Martyn J Wood
Antonis I Myridakis
Marios Andrikopoulos
Andreas Roussis
Dan Eastwood
Tariq Butt
Vassili N Kouvelis
format Journal article
container_title G3: Genes, Genomes, Genetics
container_volume 14
container_issue 10
container_start_page jkae190
publishDate 2024
institution Swansea University
issn 2160-1836
doi_str_mv 10.1093/g3journal/jkae190
publisher Oxford University Press (OUP)
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
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description Entomopathogenic fungi belonging to the Order Hypocreales are renowned for their ability to infect and kill insect hosts, while their endophytic mode of life and the beneficial rhizosphere effects on plant hosts have only been recently recognized. Understanding the molecular mechanisms underlying their different lifestyles could optimize their potential as both biocontrol and biofertilizer agents, as well as the wider appreciation of niche plasticity in fungal ecology. This study describes the comprehensive whole genome sequencing and analysis of one of the most effective entomopathogenic and endophytic EPF strains, Metarhizium brunneum V275 (commercially known as Lalguard Met52), achieved through Nanopore and Illumina reads. Comparative genomics for exploring intraspecies variability and analyses of key gene sets were conducted with a second effective EPF strain, M. brunneum ARSEF 4556. The search for strain- or species-specific genes was extended to M. brunneum strain ARSEF 3297 and other species of genus Metarhizium, to identify molecular mechanisms and putative key genome adaptations associated with mode of life differences. Genome size differed significantly, with M. brunneum V275 having the largest genome amongst M. brunneum strains sequenced to date. Genome analyses revealed an abundance of plant-degrading enzymes, plant colonization-associated genes, and intriguing intraspecies variations regarding their predicted secondary metabolic compounds and the number and localization of Transposable Elements. The potential significance of the differences found between closely related endophytic and entomopathogenic fungi, regarding plant growth-promoting and entomopathogenic abilities, are discussed, enhancing our understanding of their diverse functionalities and putative applications in agriculture and ecology.
published_date 2024-10-31T08:36:58Z
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