Journal article 71 views
Comparative genomics of Metarhizium brunneum strains V275 and ARSEF 4556: unraveling intraspecies diversity
,
Martyn J Wood,
Antonis I Myridakis,
Marios Andrikopoulos,
Andreas Roussis ,
Dan Eastwood ,
Tariq Butt ,
Vassili N Kouvelis
G3: Genes, Genomes, Genetics, Volume: 14, Issue: 10, Start page: jkae190
Swansea University Authors:
Dan Eastwood , Tariq Butt
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.1093/g3journal/jkae190
Abstract
Entomopathogenic fungi belonging to the Order Hypocreales are renowned for their ability to infect and kill insect hosts, while their endophytic mode of life and the beneficial rhizosphere effects on plant hosts have only been recently recognized. Understanding the molecular mechanisms underlying th...
| Published in: | G3: Genes, Genomes, Genetics |
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| ISSN: | 2160-1836 |
| Published: |
Oxford University Press (OUP)
2024
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa68477 |
| Abstract: |
Entomopathogenic fungi belonging to the Order Hypocreales are renowned for their ability to infect and kill insect hosts, while their endophytic mode of life and the beneficial rhizosphere effects on plant hosts have only been recently recognized. Understanding the molecular mechanisms underlying their different lifestyles could optimize their potential as both biocontrol and biofertilizer agents, as well as the wider appreciation of niche plasticity in fungal ecology. This study describes the comprehensive whole genome sequencing and analysis of one of the most effective entomopathogenic and endophytic EPF strains, Metarhizium brunneum V275 (commercially known as Lalguard Met52), achieved through Nanopore and Illumina reads. Comparative genomics for exploring intraspecies variability and analyses of key gene sets were conducted with a second effective EPF strain, M. brunneum ARSEF 4556. The search for strain- or species-specific genes was extended to M. brunneum strain ARSEF 3297 and other species of genus Metarhizium, to identify molecular mechanisms and putative key genome adaptations associated with mode of life differences. Genome size differed significantly, with M. brunneum V275 having the largest genome amongst M. brunneum strains sequenced to date. Genome analyses revealed an abundance of plant-degrading enzymes, plant colonization-associated genes, and intriguing intraspecies variations regarding their predicted secondary metabolic compounds and the number and localization of Transposable Elements. The potential significance of the differences found between closely related endophytic and entomopathogenic fungi, regarding plant growth-promoting and entomopathogenic abilities, are discussed, enhancing our understanding of their diverse functionalities and putative applications in agriculture and ecology. |
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| Keywords: |
Endophytic entomopathogenic fungi; Metarhizium brunneum; whole genome sequencing; biosynthetic gene clusters (BGCs); CAZymes; transposable elements (TEs) |
| College: |
Faculty of Science and Engineering |
| Funders: |
The research work was supported by the Hellenic Foundation for Research and Innovation (HFRI) under the 3rd Call for HFRI PhD Fellowships (Fellowship Number: 5879). |
| Issue: |
10 |
| Start Page: |
jkae190 |