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An Arabidopsis pex10 Null Mutant Is Embryo Lethal, Implicating Peroxisomes in an Essential Role during Plant Embryogenesis

Imogen A. Sparkes, Federica Brandizzi, Steve Slocombe Orcid Logo, Mahmoud El-Shami, Chris Hawes, Alison Baker

Plant Physiology, Volume: 133, Issue: 4, Pages: 1809 - 1819

Swansea University Author: Steve Slocombe Orcid Logo

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DOI (Published version): 10.1104/pp.103.031252

Abstract

Peroxisomes participate in many important functions in plants, including seed reserve mobilization, photorespiration, defense against oxidative stress, and auxin and jasmonate signaling. In mammals, defects in peroxisome biogenesis result in multiple system abnormalities, severe developmental delay,...

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Published in: Plant Physiology
ISSN: 0032-0889 1532-2548
Published: Oxford University Press (OUP) 2003
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URI: https://cronfa.swan.ac.uk/Record/cronfa65486
first_indexed 2024-01-22T14:13:41Z
last_indexed 2024-11-25T14:16:11Z
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spelling 2024-03-21T16:27:40.5352910 v2 65486 2024-01-22 An Arabidopsis pex10 Null Mutant Is Embryo Lethal, Implicating Peroxisomes in an Essential Role during Plant Embryogenesis 4a1ea486a78ed357efdfa053a277ae40 0000-0002-3549-7999 Steve Slocombe Steve Slocombe true false 2024-01-22 BGPS Peroxisomes participate in many important functions in plants, including seed reserve mobilization, photorespiration, defense against oxidative stress, and auxin and jasmonate signaling. In mammals, defects in peroxisome biogenesis result in multiple system abnormalities, severe developmental delay, and death, whereas in unicellular yeasts, peroxisomes are dispensable unless required for growth of specific substrates. PEX10 encodes an integral membrane protein required for peroxisome biogenesis in mammals and yeast. To investigate the importance of PEX10 in plants, we characterized a Ds insertion mutant in the PEX10 gene of Arabidopsis (AtPEX10). Heterozygous AtPEX10::dissociation element mutants show normal vegetative phenotypes under optimal growth conditions, but produce about 20% abnormal seeds. The embryos in the abnormal seeds are predominantly homozygous for the disruption allele. They show retarded development and some morphological abnormalities. No viable homozygous mutant plants were obtained. AtPEX10 fused to yellow fluorescent protein colocalized with green fluorescent protein-serine-lysine-leucine, a well-documented peroxisomal marker, suggesting that AtPEX10 encodes a peroxisomal protein that is essential for normal embryo development and viability. Journal Article Plant Physiology 133 4 1809 1819 Oxford University Press (OUP) 0032-0889 1532-2548 17 12 2003 2003-12-17 10.1104/pp.103.031252 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University 2024-03-21T16:27:40.5352910 2024-01-22T14:13:07.7892862 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Imogen A. Sparkes 1 Federica Brandizzi 2 Steve Slocombe 0000-0002-3549-7999 3 Mahmoud El-Shami 4 Chris Hawes 5 Alison Baker 6
title An Arabidopsis pex10 Null Mutant Is Embryo Lethal, Implicating Peroxisomes in an Essential Role during Plant Embryogenesis
spellingShingle An Arabidopsis pex10 Null Mutant Is Embryo Lethal, Implicating Peroxisomes in an Essential Role during Plant Embryogenesis
Steve Slocombe
title_short An Arabidopsis pex10 Null Mutant Is Embryo Lethal, Implicating Peroxisomes in an Essential Role during Plant Embryogenesis
title_full An Arabidopsis pex10 Null Mutant Is Embryo Lethal, Implicating Peroxisomes in an Essential Role during Plant Embryogenesis
title_fullStr An Arabidopsis pex10 Null Mutant Is Embryo Lethal, Implicating Peroxisomes in an Essential Role during Plant Embryogenesis
title_full_unstemmed An Arabidopsis pex10 Null Mutant Is Embryo Lethal, Implicating Peroxisomes in an Essential Role during Plant Embryogenesis
title_sort An Arabidopsis pex10 Null Mutant Is Embryo Lethal, Implicating Peroxisomes in an Essential Role during Plant Embryogenesis
author_id_str_mv 4a1ea486a78ed357efdfa053a277ae40
author_id_fullname_str_mv 4a1ea486a78ed357efdfa053a277ae40_***_Steve Slocombe
author Steve Slocombe
author2 Imogen A. Sparkes
Federica Brandizzi
Steve Slocombe
Mahmoud El-Shami
Chris Hawes
Alison Baker
format Journal article
container_title Plant Physiology
container_volume 133
container_issue 4
container_start_page 1809
publishDate 2003
institution Swansea University
issn 0032-0889
1532-2548
doi_str_mv 10.1104/pp.103.031252
publisher Oxford University Press (OUP)
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 0
active_str 0
description Peroxisomes participate in many important functions in plants, including seed reserve mobilization, photorespiration, defense against oxidative stress, and auxin and jasmonate signaling. In mammals, defects in peroxisome biogenesis result in multiple system abnormalities, severe developmental delay, and death, whereas in unicellular yeasts, peroxisomes are dispensable unless required for growth of specific substrates. PEX10 encodes an integral membrane protein required for peroxisome biogenesis in mammals and yeast. To investigate the importance of PEX10 in plants, we characterized a Ds insertion mutant in the PEX10 gene of Arabidopsis (AtPEX10). Heterozygous AtPEX10::dissociation element mutants show normal vegetative phenotypes under optimal growth conditions, but produce about 20% abnormal seeds. The embryos in the abnormal seeds are predominantly homozygous for the disruption allele. They show retarded development and some morphological abnormalities. No viable homozygous mutant plants were obtained. AtPEX10 fused to yellow fluorescent protein colocalized with green fluorescent protein-serine-lysine-leucine, a well-documented peroxisomal marker, suggesting that AtPEX10 encodes a peroxisomal protein that is essential for normal embryo development and viability.
published_date 2003-12-17T08:27:41Z
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score 11.544631

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