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Transcriptomic and Reverse Genetic Analysesof Branched-Chain Fatty Acid and Acyl Sugar Production in Solanum pennellii and Nicotiana benthamiana

Steve Slocombe Orcid Logo, Ines Schauvinhold, Ryan P. McQuinn, Katrin Besser, Nicholas A. Welsby, Andrea Harper, Naveed Aziz, Yi Li, Tony R. Larson, James Giovannoni, Richard A. Dixon, Pierre Broun

Plant Physiology, Volume: 148, Issue: 4, Pages: 1830 - 1846

Swansea University Author: Steve Slocombe Orcid Logo

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DOI (Published version): 10.1104/pp.108.129510

Abstract

Acyl sugars containing branched-chain fatty acids (BCFAs) are exuded by glandular trichomes of many species in Solanaceae, having an important defensive role against insects. From isotope-feeding studies, two modes of BCFA elongation have been proposed: (1) fatty acid synthase-mediated two-carbon el...

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Published in: Plant Physiology
ISSN: 1532-2548
Published: Oxford University Press (OUP) 2008
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URI: https://cronfa.swan.ac.uk/Record/cronfa65480
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last_indexed 2024-11-25T14:16:10Z
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spelling 2024-03-23T11:30:47.1891107 v2 65480 2024-01-22 Transcriptomic and Reverse Genetic Analysesof Branched-Chain Fatty Acid and Acyl Sugar Production in Solanum pennellii and Nicotiana benthamiana 4a1ea486a78ed357efdfa053a277ae40 0000-0002-3549-7999 Steve Slocombe Steve Slocombe true false 2024-01-22 BGPS Acyl sugars containing branched-chain fatty acids (BCFAs) are exuded by glandular trichomes of many species in Solanaceae, having an important defensive role against insects. From isotope-feeding studies, two modes of BCFA elongation have been proposed: (1) fatty acid synthase-mediated two-carbon elongation in the high acyl sugar-producing tomato species Solanum pennellii and Datura metel; and (2) alpha-keto acid elongation-mediated one-carbon increments in several tobacco (Nicotiana) species and a Petunia species. To investigate the molecular mechanisms underlying BCFAs and acyl sugar production in trichomes, we have taken a comparative genomic approach to identify critical enzymatic steps followed by gene silencing and metabolite analysis in S. pennellii and Nicotiana benthamiana. Our study verified the existence of distinct mechanisms of acyl sugar synthesis in Solanaceae. From microarray analyses, genes associated with alpha-keto acid elongation were found to be among the most strongly expressed in N. benthamiana trichomes only, supporting this model in tobacco species. Genes encoding components of the branched-chain keto-acid dehydrogenase complex were expressed at particularly high levels in trichomes of both species, and we show using virus-induced gene silencing that they are required for BCFA production in both cases and for acyl sugar synthesis in N. benthamiana. Functional analysis by down-regulation of specific KAS I genes and cerulenin inhibition indicated the involvement of the fatty acid synthase complex in BCFA production in S. pennellii. In summary, our study highlights both conserved and divergent mechanisms in the production of important defense compounds in Solanaceae and defines potential targets for engineering acyl sugar production in plants for improved pest tolerance. Journal Article Plant Physiology 148 4 1830 1846 Oxford University Press (OUP) 1532-2548 4 12 2008 2008-12-04 10.1104/pp.108.129510 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University 2024-03-23T11:30:47.1891107 2024-01-22T13:30:34.6217270 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Steve Slocombe 0000-0002-3549-7999 1 Ines Schauvinhold 2 Ryan P. McQuinn 3 Katrin Besser 4 Nicholas A. Welsby 5 Andrea Harper 6 Naveed Aziz 7 Yi Li 8 Tony R. Larson 9 James Giovannoni 10 Richard A. Dixon 11 Pierre Broun 12
title Transcriptomic and Reverse Genetic Analysesof Branched-Chain Fatty Acid and Acyl Sugar Production in Solanum pennellii and Nicotiana benthamiana
spellingShingle Transcriptomic and Reverse Genetic Analysesof Branched-Chain Fatty Acid and Acyl Sugar Production in Solanum pennellii and Nicotiana benthamiana
Steve Slocombe
title_short Transcriptomic and Reverse Genetic Analysesof Branched-Chain Fatty Acid and Acyl Sugar Production in Solanum pennellii and Nicotiana benthamiana
title_full Transcriptomic and Reverse Genetic Analysesof Branched-Chain Fatty Acid and Acyl Sugar Production in Solanum pennellii and Nicotiana benthamiana
title_fullStr Transcriptomic and Reverse Genetic Analysesof Branched-Chain Fatty Acid and Acyl Sugar Production in Solanum pennellii and Nicotiana benthamiana
title_full_unstemmed Transcriptomic and Reverse Genetic Analysesof Branched-Chain Fatty Acid and Acyl Sugar Production in Solanum pennellii and Nicotiana benthamiana
title_sort Transcriptomic and Reverse Genetic Analysesof Branched-Chain Fatty Acid and Acyl Sugar Production in Solanum pennellii and Nicotiana benthamiana
author_id_str_mv 4a1ea486a78ed357efdfa053a277ae40
author_id_fullname_str_mv 4a1ea486a78ed357efdfa053a277ae40_***_Steve Slocombe
author Steve Slocombe
author2 Steve Slocombe
Ines Schauvinhold
Ryan P. McQuinn
Katrin Besser
Nicholas A. Welsby
Andrea Harper
Naveed Aziz
Yi Li
Tony R. Larson
James Giovannoni
Richard A. Dixon
Pierre Broun
format Journal article
container_title Plant Physiology
container_volume 148
container_issue 4
container_start_page 1830
publishDate 2008
institution Swansea University
issn 1532-2548
doi_str_mv 10.1104/pp.108.129510
publisher Oxford University Press (OUP)
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 0
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description Acyl sugars containing branched-chain fatty acids (BCFAs) are exuded by glandular trichomes of many species in Solanaceae, having an important defensive role against insects. From isotope-feeding studies, two modes of BCFA elongation have been proposed: (1) fatty acid synthase-mediated two-carbon elongation in the high acyl sugar-producing tomato species Solanum pennellii and Datura metel; and (2) alpha-keto acid elongation-mediated one-carbon increments in several tobacco (Nicotiana) species and a Petunia species. To investigate the molecular mechanisms underlying BCFAs and acyl sugar production in trichomes, we have taken a comparative genomic approach to identify critical enzymatic steps followed by gene silencing and metabolite analysis in S. pennellii and Nicotiana benthamiana. Our study verified the existence of distinct mechanisms of acyl sugar synthesis in Solanaceae. From microarray analyses, genes associated with alpha-keto acid elongation were found to be among the most strongly expressed in N. benthamiana trichomes only, supporting this model in tobacco species. Genes encoding components of the branched-chain keto-acid dehydrogenase complex were expressed at particularly high levels in trichomes of both species, and we show using virus-induced gene silencing that they are required for BCFA production in both cases and for acyl sugar synthesis in N. benthamiana. Functional analysis by down-regulation of specific KAS I genes and cerulenin inhibition indicated the involvement of the fatty acid synthase complex in BCFA production in S. pennellii. In summary, our study highlights both conserved and divergent mechanisms in the production of important defense compounds in Solanaceae and defines potential targets for engineering acyl sugar production in plants for improved pest tolerance.
published_date 2008-12-04T08:27:40Z
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