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Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways

Steve Slocombe, Jo Cornah, Helen Pinfield‐Wells, Kelly Soady, QianYi Zhang, Alison Gilday, John M. Dyer, Ian A. Graham

Plant Biotechnology Journal, Volume: 7, Issue: 7, Pages: 694 - 703

Swansea University Author: Steve Slocombe

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DOI (Published version): 10.1111/j.1467-7652.2009.00435.x

Abstract

Plant oils in the form of triacylglycerol (TAG) are used for food, industrial feedstock and biofuel manufacture. Although TAG is typically harvested from the fruit or seeds of oil crop species, plants can also accumulate small amounts of TAG in the leaves and other vegetative tissues. Here we show t...

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Published in: Plant Biotechnology Journal
ISSN: 1467-7644 1467-7652
Published: Wiley 2009
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URI: https://cronfa.swan.ac.uk/Record/cronfa65478
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spelling v2 65478 2024-01-22 Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways 4a1ea486a78ed357efdfa053a277ae40 Steve Slocombe Steve Slocombe true false 2024-01-22 SBI Plant oils in the form of triacylglycerol (TAG) are used for food, industrial feedstock and biofuel manufacture. Although TAG is typically harvested from the fruit or seeds of oil crop species, plants can also accumulate small amounts of TAG in the leaves and other vegetative tissues. Here we show that leaf TAG levels can be increased significantly (10–20 fold) by blocking fatty acid breakdown, particularly during extended dark treatments or leaf senescence in the model plant Arabidopsis. Generation of a double mutant in fatty acid breakdown and diacylglycerol acyltransferase 1 (DGAT1) resulted in a severe vegetative growth phenotype suggesting that partitioning of fatty acids to TAG in leaves is carried out predominantly by this acyltransferase. LEC2, a seed development transcription factor involved in storage product accumulation, was ectopically expressed during senescence in the fatty acid breakdown mutant COMATOSE (cts2). This resulted in accumulation of seed oil type species of TAG in senescing tissue. Our data suggests that recycled membrane fatty acids can be re-directed to TAG by expressing the seed-programme in senescing tissue or by a block in fatty acid breakdown. This work raises the possibility of producing significant amounts of oil in vegetative tissues of biomass crops such as Miscanthus. Journal Article Plant Biotechnology Journal 7 7 694 703 Wiley 1467-7644 1467-7652 biofuel, triacylglycerol, beta-oxidation, diacylglycerol acyl-transferase 1, LEC 1 9 2009 2009-09-01 10.1111/j.1467-7652.2009.00435.x COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University Another institution paid the OA fee 2024-03-23T11:33:20.0255588 2024-01-22T13:12:53.7707069 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Steve Slocombe 1 Jo Cornah 2 Helen Pinfield‐Wells 3 Kelly Soady 4 QianYi Zhang 5 Alison Gilday 6 John M. Dyer 7 Ian A. Graham 8
title Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways
spellingShingle Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways
Steve Slocombe
title_short Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways
title_full Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways
title_fullStr Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways
title_full_unstemmed Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways
title_sort Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways
author_id_str_mv 4a1ea486a78ed357efdfa053a277ae40
author_id_fullname_str_mv 4a1ea486a78ed357efdfa053a277ae40_***_Steve Slocombe
author Steve Slocombe
author2 Steve Slocombe
Jo Cornah
Helen Pinfield‐Wells
Kelly Soady
QianYi Zhang
Alison Gilday
John M. Dyer
Ian A. Graham
format Journal article
container_title Plant Biotechnology Journal
container_volume 7
container_issue 7
container_start_page 694
publishDate 2009
institution Swansea University
issn 1467-7644
1467-7652
doi_str_mv 10.1111/j.1467-7652.2009.00435.x
publisher Wiley
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 0
active_str 0
description Plant oils in the form of triacylglycerol (TAG) are used for food, industrial feedstock and biofuel manufacture. Although TAG is typically harvested from the fruit or seeds of oil crop species, plants can also accumulate small amounts of TAG in the leaves and other vegetative tissues. Here we show that leaf TAG levels can be increased significantly (10–20 fold) by blocking fatty acid breakdown, particularly during extended dark treatments or leaf senescence in the model plant Arabidopsis. Generation of a double mutant in fatty acid breakdown and diacylglycerol acyltransferase 1 (DGAT1) resulted in a severe vegetative growth phenotype suggesting that partitioning of fatty acids to TAG in leaves is carried out predominantly by this acyltransferase. LEC2, a seed development transcription factor involved in storage product accumulation, was ectopically expressed during senescence in the fatty acid breakdown mutant COMATOSE (cts2). This resulted in accumulation of seed oil type species of TAG in senescing tissue. Our data suggests that recycled membrane fatty acids can be re-directed to TAG by expressing the seed-programme in senescing tissue or by a block in fatty acid breakdown. This work raises the possibility of producing significant amounts of oil in vegetative tissues of biomass crops such as Miscanthus.
published_date 2009-09-01T11:33:17Z
_version_ 1794316537366052864
score 11.013507

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