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Mass Spectrometry Imaging of Cholesterol and Oxysterols

William Griffiths Orcid Logo, Eylan Yutuc Orcid Logo, Yuqin Wang Orcid Logo

Implication of Oxysterols and Phytosterols in Aging and Human Diseases, Pages: 73 - 87

Swansea University Authors: William Griffiths Orcid Logo, Eylan Yutuc Orcid Logo, Yuqin Wang Orcid Logo

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DOI (Published version): 10.1007/978-3-031-43883-7_5

Abstract

Mass spectrometry imaging (MSI) is a new technique in the toolbox of the analytical biochemist. It allows the generation of a compound specific image from a tissue slice where a measure of compound abundance is given pixel by pixel, usually displayed on a colour scale. As mass spectra are recorded a...

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Published in: Implication of Oxysterols and Phytosterols in Aging and Human Diseases
ISBN: 9783031438820 9783031438837
ISSN: 0065-2598 2214-8019
Published: Cham Springer International Publishing 2023
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URI: https://cronfa.swan.ac.uk/Record/cronfa64898
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spelling v2 64898 2023-11-02 Mass Spectrometry Imaging of Cholesterol and Oxysterols 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 99332f073ce913a9b7d8b6441b17516d 0000-0001-9971-1950 Eylan Yutuc Eylan Yutuc true false c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 2023-11-02 BMS Mass spectrometry imaging (MSI) is a new technique in the toolbox of the analytical biochemist. It allows the generation of a compound specific image from a tissue slice where a measure of compound abundance is given pixel by pixel, usually displayed on a colour scale. As mass spectra are recorded at each pixel the data can be interrogated to generate images of multiple different compounds all in the same experiment. Mass spectrometry (MS) requires ionisation of analytes but cholesterol and other neutral sterols tend to be poorly ionised by the techniques employed in most MSI experiments, so despite its high abundance in mammalian tissues cholesterol is poorly represented in the MSI literature. In this article we discuss some of the MSI studies where cholesterol has been imaged and introduce newer methods for its analysis by MSI. Disturbed cholesterol metabolism is linked to many disorders and the potential of MSI to study cholesterol, its precursors and its metabolites in animal models and from human biopsies will be discussed. Book chapter Implication of Oxysterols and Phytosterols in Aging and Human Diseases 73 87 Springer International Publishing Cham 9783031438820 9783031438837 0065-2598 2214-8019 Mass spectrometry, mass spectrometry imaging, cholesterol, oxysterol, neurodegeneration, 24S-hydroxycholesterol 1 12 2023 2023-12-01 10.1007/978-3-031-43883-7_5 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University Not Required UKRI BB/T018542/1, BB/N015932/1, BB/S019588/1 2024-03-13T14:08:48.6468836 2023-11-02T08:37:17.6882807 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science William Griffiths 0000-0002-4129-6616 1 Eylan Yutuc 0000-0001-9971-1950 2 Yuqin Wang 0000-0002-3063-3066 3 64898__28912__35983b5f0aba4b038d800daf3c7dec95.pdf 230501_WJG_Combined (1).pdf 2023-11-02T09:04:04.5284727 Output 1487911 application/pdf Accepted Manuscript true true eng
title Mass Spectrometry Imaging of Cholesterol and Oxysterols
spellingShingle Mass Spectrometry Imaging of Cholesterol and Oxysterols
William Griffiths
Eylan Yutuc
Yuqin Wang
title_short Mass Spectrometry Imaging of Cholesterol and Oxysterols
title_full Mass Spectrometry Imaging of Cholesterol and Oxysterols
title_fullStr Mass Spectrometry Imaging of Cholesterol and Oxysterols
title_full_unstemmed Mass Spectrometry Imaging of Cholesterol and Oxysterols
title_sort Mass Spectrometry Imaging of Cholesterol and Oxysterols
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
99332f073ce913a9b7d8b6441b17516d
c92729b58622f9fdf6a0e7d8f4ce5081
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William Griffiths
99332f073ce913a9b7d8b6441b17516d_***_Eylan Yutuc
c92729b58622f9fdf6a0e7d8f4ce5081_***_Yuqin Wang
author William Griffiths
Eylan Yutuc
Yuqin Wang
author2 William Griffiths
Eylan Yutuc
Yuqin Wang
format Book chapter
container_title Implication of Oxysterols and Phytosterols in Aging and Human Diseases
container_start_page 73
publishDate 2023
institution Swansea University
isbn 9783031438820
9783031438837
issn 0065-2598
2214-8019
doi_str_mv 10.1007/978-3-031-43883-7_5
publisher Springer International Publishing
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
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description Mass spectrometry imaging (MSI) is a new technique in the toolbox of the analytical biochemist. It allows the generation of a compound specific image from a tissue slice where a measure of compound abundance is given pixel by pixel, usually displayed on a colour scale. As mass spectra are recorded at each pixel the data can be interrogated to generate images of multiple different compounds all in the same experiment. Mass spectrometry (MS) requires ionisation of analytes but cholesterol and other neutral sterols tend to be poorly ionised by the techniques employed in most MSI experiments, so despite its high abundance in mammalian tissues cholesterol is poorly represented in the MSI literature. In this article we discuss some of the MSI studies where cholesterol has been imaged and introduce newer methods for its analysis by MSI. Disturbed cholesterol metabolism is linked to many disorders and the potential of MSI to study cholesterol, its precursors and its metabolites in animal models and from human biopsies will be discussed.
published_date 2023-12-01T14:08:45Z
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