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Mass Spectrometry Imaging of Cholesterol and Oxysterols
William Griffiths 
,
Eylan Yutuc ,
Yuqin Wang
,
Eylan Yutuc ,
Yuqin Wang
Implication of Oxysterols and Phytosterols in Aging and Human Diseases, Pages: 73 - 87
Swansea University Authors:
William Griffiths , Eylan Yutuc , Yuqin Wang
-
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DOI (Published version): 10.1007/978-3-031-43883-7_5
Abstract
Mass spectrometry imaging (MSI) is a new technique in the toolbox of the analytical biochemist. It allows the generation of a compound specific image from a tissue slice where a measure of compound abundance is given pixel by pixel, usually displayed on a colour scale. As mass spectra are recorded a...
| Published in: | Implication of Oxysterols and Phytosterols in Aging and Human Diseases |
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| ISBN: | 9783031438820 9783031438837 |
| ISSN: | 0065-2598 2214-8019 |
| Published: |
Cham
Springer International Publishing
2023
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa64898 |
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| Abstract: |
Mass spectrometry imaging (MSI) is a new technique in the toolbox of the analytical biochemist. It allows the generation of a compound specific image from a tissue slice where a measure of compound abundance is given pixel by pixel, usually displayed on a colour scale. As mass spectra are recorded at each pixel the data can be interrogated to generate images of multiple different compounds all in the same experiment. Mass spectrometry (MS) requires ionisation of analytes but cholesterol and other neutral sterols tend to be poorly ionised by the techniques employed in most MSI experiments, so despite its high abundance in mammalian tissues cholesterol is poorly represented in the MSI literature. In this article we discuss some of the MSI studies where cholesterol has been imaged and introduce newer methods for its analysis by MSI. Disturbed cholesterol metabolism is linked to many disorders and the potential of MSI to study cholesterol, its precursors and its metabolites in animal models and from human biopsies will be discussed. |
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| Keywords: |
Mass spectrometry, mass spectrometry imaging, cholesterol, oxysterol, neurodegeneration, 24S-hydroxycholesterol |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Funders: |
UKRI |
| Start Page: |
73 |
| End Page: |
87 |