No Cover Image

Journal article 141 views 25 downloads

Scaffold Flexibility Controls Binding of Herpes Simplex Virus Type 1 with Sulfated Dendritic Polyglycerol Hydrogels Fabricated by Thiol‐Maleimide Click Reaction

Boonya Thongrom, Antara Sharma, Chuanxiong Nie Orcid Logo, Elisa Quaas, Marwin Raue, Sumati Bhatia Orcid Logo, Rainer Haag Orcid Logo

Macromolecular Bioscience, Volume: 22, Issue: 5

Swansea University Author: Sumati Bhatia Orcid Logo

  • 64856.VOR.pdf

    PDF | Version of Record

    © 2022 The Authors. Macromolecular Bioscience published by Wiley-VCH GmbH. Distributed under the terms of a Creative Commons Attribution Non Commercial 4.0 License (CC BY-NC 4.0).

    Download (1.48MB)

Check full text

DOI (Published version): 10.1002/mabi.202100507

Abstract

Herpes Simplex Virus-1 (HSV-1) with a diameter of 155–240 nm uses electrostatic interactions to bind with the heparan sulfate present on the cell surface to initiate infection. In this work, the initial contact using polysulfate-functionalized hydrogels is aimed to deter. The hydrogels provide a lar...

Full description

Published in: Macromolecular Bioscience
ISSN: 1616-5187 1616-5195
Published: Wiley 2022
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa64856
Tags: Add Tag
No Tags, Be the first to tag this record!
first_indexed 2023-11-17T18:23:55Z
last_indexed 2023-11-17T18:23:55Z
id cronfa64856
recordtype SURis
fullrecord <?xml version="1.0" encoding="utf-8"?><rfc1807 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xsd="http://www.w3.org/2001/XMLSchema"><bib-version>v2</bib-version><id>64856</id><entry>2023-11-01</entry><title>Scaffold Flexibility Controls Binding of Herpes Simplex Virus Type 1 with Sulfated Dendritic Polyglycerol Hydrogels Fabricated by Thiol‐Maleimide Click Reaction</title><swanseaauthors><author><sid>a6b1181ebdbe42bd03b24cbdb559d082</sid><ORCID>0000-0002-5123-4937</ORCID><firstname>Sumati</firstname><surname>Bhatia</surname><name>Sumati Bhatia</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2023-11-01</date><deptcode>CHEM</deptcode><abstract>Herpes Simplex Virus-1 (HSV-1) with a diameter of 155–240 nm uses electrostatic interactions to bind with the heparan sulfate present on the cell surface to initiate infection. In this work, the initial contact using polysulfate-functionalized hydrogels is aimed to deter. The hydrogels provide a large contact surface area for viral interaction and sulfated hydrogels are good mimics for the native heparan sulfate. In this work, hydrogels of different flexibilities are synthesized, determined by rheology. Gels are prepared within an elastic modulus range of 10–1119 Pa with a mesh size of 80–15 nm, respectively. The virus binding studies carried out with the plaque assay show that the most flexible sulfated hydrogel performs the best in binding HSV viruses. These studies prove that polysulfated hydrogels are a viable option as HSV-1 antiviral compounds. Furthermore, such hydrogel networks are also physically similar to naturally occurring mucus gels and therefore may be used as mucus substitutes.</abstract><type>Journal Article</type><journal>Macromolecular Bioscience</journal><volume>22</volume><journalNumber>5</journalNumber><paginationStart/><paginationEnd/><publisher>Wiley</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>1616-5187</issnPrint><issnElectronic>1616-5195</issnElectronic><keywords>Click chemistry, HSV-1, hydrogel, polysulfates, rheology, virus binding</keywords><publishedDay>15</publishedDay><publishedMonth>5</publishedMonth><publishedYear>2022</publishedYear><publishedDate>2022-05-15</publishedDate><doi>10.1002/mabi.202100507</doi><url>http://dx.doi.org/10.1002/mabi.202100507</url><notes/><college>COLLEGE NANME</college><department>Chemistry</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>CHEM</DepartmentCode><institution>Swansea University</institution><apcterm>Other</apcterm><funders>The study was funded by the Helmholtz Graduate School of Macromolecular Bioscience, by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—SFB 1449—431232613; subprojects A01, B03, C04, Z02 and by the German Federal Ministry of Education and Research (82DZL0098B1). SB acknowledges the financial support by DFG-project number 458564133. Open access funding enabled and organized by Projekt DEAL.</funders><projectreference/><lastEdited>2023-12-04T13:05:55.5083734</lastEdited><Created>2023-11-01T10:33:52.4473755</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Engineering and Applied Sciences - Chemistry</level></path><authors><author><firstname>Boonya</firstname><surname>Thongrom</surname><order>1</order></author><author><firstname>Antara</firstname><surname>Sharma</surname><order>2</order></author><author><firstname>Chuanxiong</firstname><surname>Nie</surname><orcid>0000-0001-7963-1187</orcid><order>3</order></author><author><firstname>Elisa</firstname><surname>Quaas</surname><order>4</order></author><author><firstname>Marwin</firstname><surname>Raue</surname><order>5</order></author><author><firstname>Sumati</firstname><surname>Bhatia</surname><orcid>0000-0002-5123-4937</orcid><order>6</order></author><author><firstname>Rainer</firstname><surname>Haag</surname><orcid>0000-0003-3840-162x</orcid><order>7</order></author></authors><documents><document><filename>64856__29182__0cc08295b1704a7b8e8da99721703a4f.pdf</filename><originalFilename>64856.VOR.pdf</originalFilename><uploaded>2023-12-04T13:04:14.0002901</uploaded><type>Output</type><contentLength>1554045</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>© 2022 The Authors. Macromolecular Bioscience published by Wiley-VCH GmbH. Distributed under the terms of a Creative Commons Attribution Non Commercial 4.0 License (CC BY-NC 4.0).</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>https://creativecommons.org/licenses/by-nc/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling v2 64856 2023-11-01 Scaffold Flexibility Controls Binding of Herpes Simplex Virus Type 1 with Sulfated Dendritic Polyglycerol Hydrogels Fabricated by Thiol‐Maleimide Click Reaction a6b1181ebdbe42bd03b24cbdb559d082 0000-0002-5123-4937 Sumati Bhatia Sumati Bhatia true false 2023-11-01 CHEM Herpes Simplex Virus-1 (HSV-1) with a diameter of 155–240 nm uses electrostatic interactions to bind with the heparan sulfate present on the cell surface to initiate infection. In this work, the initial contact using polysulfate-functionalized hydrogels is aimed to deter. The hydrogels provide a large contact surface area for viral interaction and sulfated hydrogels are good mimics for the native heparan sulfate. In this work, hydrogels of different flexibilities are synthesized, determined by rheology. Gels are prepared within an elastic modulus range of 10–1119 Pa with a mesh size of 80–15 nm, respectively. The virus binding studies carried out with the plaque assay show that the most flexible sulfated hydrogel performs the best in binding HSV viruses. These studies prove that polysulfated hydrogels are a viable option as HSV-1 antiviral compounds. Furthermore, such hydrogel networks are also physically similar to naturally occurring mucus gels and therefore may be used as mucus substitutes. Journal Article Macromolecular Bioscience 22 5 Wiley 1616-5187 1616-5195 Click chemistry, HSV-1, hydrogel, polysulfates, rheology, virus binding 15 5 2022 2022-05-15 10.1002/mabi.202100507 http://dx.doi.org/10.1002/mabi.202100507 COLLEGE NANME Chemistry COLLEGE CODE CHEM Swansea University Other The study was funded by the Helmholtz Graduate School of Macromolecular Bioscience, by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—SFB 1449—431232613; subprojects A01, B03, C04, Z02 and by the German Federal Ministry of Education and Research (82DZL0098B1). SB acknowledges the financial support by DFG-project number 458564133. Open access funding enabled and organized by Projekt DEAL. 2023-12-04T13:05:55.5083734 2023-11-01T10:33:52.4473755 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemistry Boonya Thongrom 1 Antara Sharma 2 Chuanxiong Nie 0000-0001-7963-1187 3 Elisa Quaas 4 Marwin Raue 5 Sumati Bhatia 0000-0002-5123-4937 6 Rainer Haag 0000-0003-3840-162x 7 64856__29182__0cc08295b1704a7b8e8da99721703a4f.pdf 64856.VOR.pdf 2023-12-04T13:04:14.0002901 Output 1554045 application/pdf Version of Record true © 2022 The Authors. Macromolecular Bioscience published by Wiley-VCH GmbH. Distributed under the terms of a Creative Commons Attribution Non Commercial 4.0 License (CC BY-NC 4.0). true eng https://creativecommons.org/licenses/by-nc/4.0/
title Scaffold Flexibility Controls Binding of Herpes Simplex Virus Type 1 with Sulfated Dendritic Polyglycerol Hydrogels Fabricated by Thiol‐Maleimide Click Reaction
spellingShingle Scaffold Flexibility Controls Binding of Herpes Simplex Virus Type 1 with Sulfated Dendritic Polyglycerol Hydrogels Fabricated by Thiol‐Maleimide Click Reaction
Sumati Bhatia
title_short Scaffold Flexibility Controls Binding of Herpes Simplex Virus Type 1 with Sulfated Dendritic Polyglycerol Hydrogels Fabricated by Thiol‐Maleimide Click Reaction
title_full Scaffold Flexibility Controls Binding of Herpes Simplex Virus Type 1 with Sulfated Dendritic Polyglycerol Hydrogels Fabricated by Thiol‐Maleimide Click Reaction
title_fullStr Scaffold Flexibility Controls Binding of Herpes Simplex Virus Type 1 with Sulfated Dendritic Polyglycerol Hydrogels Fabricated by Thiol‐Maleimide Click Reaction
title_full_unstemmed Scaffold Flexibility Controls Binding of Herpes Simplex Virus Type 1 with Sulfated Dendritic Polyglycerol Hydrogels Fabricated by Thiol‐Maleimide Click Reaction
title_sort Scaffold Flexibility Controls Binding of Herpes Simplex Virus Type 1 with Sulfated Dendritic Polyglycerol Hydrogels Fabricated by Thiol‐Maleimide Click Reaction
author_id_str_mv a6b1181ebdbe42bd03b24cbdb559d082
author_id_fullname_str_mv a6b1181ebdbe42bd03b24cbdb559d082_***_Sumati Bhatia
author Sumati Bhatia
author2 Boonya Thongrom
Antara Sharma
Chuanxiong Nie
Elisa Quaas
Marwin Raue
Sumati Bhatia
Rainer Haag
format Journal article
container_title Macromolecular Bioscience
container_volume 22
container_issue 5
publishDate 2022
institution Swansea University
issn 1616-5187
1616-5195
doi_str_mv 10.1002/mabi.202100507
publisher Wiley
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemistry{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemistry
url http://dx.doi.org/10.1002/mabi.202100507
document_store_str 1
active_str 0
description Herpes Simplex Virus-1 (HSV-1) with a diameter of 155–240 nm uses electrostatic interactions to bind with the heparan sulfate present on the cell surface to initiate infection. In this work, the initial contact using polysulfate-functionalized hydrogels is aimed to deter. The hydrogels provide a large contact surface area for viral interaction and sulfated hydrogels are good mimics for the native heparan sulfate. In this work, hydrogels of different flexibilities are synthesized, determined by rheology. Gels are prepared within an elastic modulus range of 10–1119 Pa with a mesh size of 80–15 nm, respectively. The virus binding studies carried out with the plaque assay show that the most flexible sulfated hydrogel performs the best in binding HSV viruses. These studies prove that polysulfated hydrogels are a viable option as HSV-1 antiviral compounds. Furthermore, such hydrogel networks are also physically similar to naturally occurring mucus gels and therefore may be used as mucus substitutes.
published_date 2022-05-15T13:05:56Z
_version_ 1784356700679045120
score 11.013731

Similar Items