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Scaffold Flexibility Controls Binding of Herpes Simplex Virus Type 1 with Sulfated Dendritic Polyglycerol Hydrogels Fabricated by Thiol‐Maleimide Click Reaction

Boonya Thongrom, Antara Sharma, Chuanxiong Nie Orcid Logo, Elisa Quaas, Marwin Raue, Sumati Bhatia Orcid Logo, Rainer Haag Orcid Logo

Macromolecular Bioscience, Volume: 22, Issue: 5

Swansea University Author: Sumati Bhatia Orcid Logo

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DOI (Published version): 10.1002/mabi.202100507

Abstract

Herpes Simplex Virus-1 (HSV-1) with a diameter of 155–240 nm uses electrostatic interactions to bind with the heparan sulfate present on the cell surface to initiate infection. In this work, the initial contact using polysulfate-functionalized hydrogels is aimed to deter. The hydrogels provide a lar...

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Published in: Macromolecular Bioscience
ISSN: 1616-5187 1616-5195
Published: Wiley 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa64856
Abstract: Herpes Simplex Virus-1 (HSV-1) with a diameter of 155–240 nm uses electrostatic interactions to bind with the heparan sulfate present on the cell surface to initiate infection. In this work, the initial contact using polysulfate-functionalized hydrogels is aimed to deter. The hydrogels provide a large contact surface area for viral interaction and sulfated hydrogels are good mimics for the native heparan sulfate. In this work, hydrogels of different flexibilities are synthesized, determined by rheology. Gels are prepared within an elastic modulus range of 10–1119 Pa with a mesh size of 80–15 nm, respectively. The virus binding studies carried out with the plaque assay show that the most flexible sulfated hydrogel performs the best in binding HSV viruses. These studies prove that polysulfated hydrogels are a viable option as HSV-1 antiviral compounds. Furthermore, such hydrogel networks are also physically similar to naturally occurring mucus gels and therefore may be used as mucus substitutes.
Keywords: Click chemistry, HSV-1, hydrogel, polysulfates, rheology, virus binding
College: Faculty of Science and Engineering
Funders: The study was funded by the Helmholtz Graduate School of Macromolecular Bioscience, by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—SFB 1449—431232613; subprojects A01, B03, C04, Z02 and by the German Federal Ministry of Education and Research (82DZL0098B1). SB acknowledges the financial support by DFG-project number 458564133. Open access funding enabled and organized by Projekt DEAL.
Issue: 5

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