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A comparative analysis of pulp-derived nanocelluloses for 3D bioprinting facial cartilages
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Kim Nelson,
Shareen Doak ,
Iain Whitaker
Carbohydrate Polymers, Volume: 321, Start page: 121261
Swansea University Authors:
Thomas Jovic, Hari Arora , Shareen Doak , Iain Whitaker
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DOI (Published version): 10.1016/j.carbpol.2023.121261
Abstract
Nanocelluloses have attracted significant interest in the field of bioprinting, with previous research outlining the value of nanocellulose fibrils and bacterial nanocelluloses for 3D bioprinting tissues such as cartilage. We have recently characterised three distinct structural formulations of pulp...
| Published in: | Carbohydrate Polymers |
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| ISSN: | 0144-8617 1879-1344 |
| Published: |
Elsevier BV
2023
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa64062 |
| Abstract: |
Nanocelluloses have attracted significant interest in the field of bioprinting, with previous research outlining the value of nanocellulose fibrils and bacterial nanocelluloses for 3D bioprinting tissues such as cartilage. We have recently characterised three distinct structural formulations of pulp-derived nanocelluloses: fibrillar (NFC), crystalline (NCC) and blend (NCB), exhibiting variation in pore geometry and mechanical properties. In light of the characterisation of these three distinct entities, this study investigated whether these structural differences translated to differences in printability, chondrogenicity or biocompatibility for 3D bioprinting anatomical structures with human nasoseptal chondrocytes. Composite nanocellulose-alginate bioinks (75:25 v/v) of NFC, NCC and NCB were produced and tested for print resolution and fidelity. NFC offered superior print resolution whereas NCB demonstrated the best post-printing shape fidelity. Biologically, chondrogenicity was assessed using real time quantitative PCR, dimethylmethylene blue assays and histology. All biomaterials showed an increase in chondrogenic gene expression and extracellular matrix production over 21 days, but this was superior in the NCC bioink. Biocompatibility assessments revealed an increase in cell number and metabolism over 21 days in the NCC and NCB formulations. Nanocellulose augments printability and chondrogenicity of bioinks, of which the NCC and NCB formulations offer the best biological promise for bioprinting cartilage. |
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| Keywords: |
Cartilage, Bioprinting, Nanocellulose, Alginate |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Funders: |
This study was supported by The Scar Free Foundation & Health and Care Research Wales Programme of research in Reconstructive Surgery & Regenerative Medicine, which has been established in the ReconRegen Research Centre at Swansea University in partnership with Swansea Bay University Health Board. This work was additionally supported by Action Medical Research and the VTCT Foundation (Grant Number 2782), the Royal College of Surgeons England and the Welsh Clinical Academic Training Programme. |
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121261 |