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Gene regulation in fatty acid pathways in cyanobacteria exposed to far-red light / PATRICK COLLEDGE

Swansea University Author: PATRICK COLLEDGE

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Abstract

Cyanobacteria are photosynthetic microorganisms that can utilise white, far-red or UV light. The organisms adapt their cellular metabolism to their environment by regulating their genetic expression to accumulate or reduce metabolites such as valuable polyunsaturated fatty acids. The production of h...

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Published: Swansea, Wales, UK 2023
Institution: Swansea University
Degree level: Master of Research
Degree name: MRes
Supervisor: Lurgi, Miguel
URI: https://cronfa.swan.ac.uk/Record/cronfa63577
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first_indexed 2023-06-02T16:20:26Z
last_indexed 2023-06-02T16:20:26Z
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spelling v2 63577 2023-06-02 Gene regulation in fatty acid pathways in cyanobacteria exposed to far-red light 74f210b8a944f63365bc69ee696c024f PATRICK COLLEDGE PATRICK COLLEDGE true false 2023-06-02 Cyanobacteria are photosynthetic microorganisms that can utilise white, far-red or UV light. The organisms adapt their cellular metabolism to their environment by regulating their genetic expression to accumulate or reduce metabolites such as valuable polyunsaturated fatty acids. The production of high-value metabolites whilst using light and CO2 to grow make these organisms a successful candidate within the biotechnological industry. Far-red light is known to influence cyanobacteria, especially their photosynthetic apparatus. Thylakoid membranes are known to be altered by far-red light photoacclimation (FaRLiP). However, the role of fatty acid synthesis and desaturation during FarLip is little studied. This study examines the gene expression of 33 fatty acid-related genes when the cyanobacterium Chlorogloeopsis fritschii PCC 6912 is grown under far-red light compared to white light using RNA-seq data from the NCBI database. The transcriptomic analysis, encompassing a bioinformatic pipeline to process and quantify transcripts, found that only two genes of the 33 examined genes were differentially expressed. The two genes were down-regulated and encoded for a fatty acid desaturase (fad) and a pfaD/polyketide biosynthesis protein. The results suggest that fatty acid desaturation decreases in favour of saturated fatty acids. Furthermore, the identification of a pfa gene cluster may highlight a secondary path of polyunsaturated fatty acids within heterocyst cyanobacteria. This study also highlights the need for greater biological replicates within RNA-seq experiments and increased completeness in genome annotations in biotechnological databases. E-Thesis Swansea, Wales, UK Cyanobacteria, fatty acid, RNA-seq, gene expression, microalgae 10 5 2023 2023-05-10 A selection of content is redacted or is partially redacted from this thesis to protect sensitive and personal information. COLLEGE NANME COLLEGE CODE Swansea University Lurgi, Miguel Master of Research MRes Student Finance England 2023-10-27T16:05:12.8172992 2023-06-02T17:17:31.6531626 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences PATRICK COLLEDGE 1 63577__27692__387fd2da550e4bac9ad481f7f1211817.pdf 2023_Colledge_PW.final.63577.pdf 2023-06-02T17:21:29.6087947 Output 2477322 application/pdf E-Thesis – open access true Copyright: The Author, Patrick W. Colledge, 2023. true eng
title Gene regulation in fatty acid pathways in cyanobacteria exposed to far-red light
spellingShingle Gene regulation in fatty acid pathways in cyanobacteria exposed to far-red light
PATRICK COLLEDGE
title_short Gene regulation in fatty acid pathways in cyanobacteria exposed to far-red light
title_full Gene regulation in fatty acid pathways in cyanobacteria exposed to far-red light
title_fullStr Gene regulation in fatty acid pathways in cyanobacteria exposed to far-red light
title_full_unstemmed Gene regulation in fatty acid pathways in cyanobacteria exposed to far-red light
title_sort Gene regulation in fatty acid pathways in cyanobacteria exposed to far-red light
author_id_str_mv 74f210b8a944f63365bc69ee696c024f
author_id_fullname_str_mv 74f210b8a944f63365bc69ee696c024f_***_PATRICK COLLEDGE
author PATRICK COLLEDGE
author2 PATRICK COLLEDGE
format E-Thesis
publishDate 2023
institution Swansea University
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
active_str 0
description Cyanobacteria are photosynthetic microorganisms that can utilise white, far-red or UV light. The organisms adapt their cellular metabolism to their environment by regulating their genetic expression to accumulate or reduce metabolites such as valuable polyunsaturated fatty acids. The production of high-value metabolites whilst using light and CO2 to grow make these organisms a successful candidate within the biotechnological industry. Far-red light is known to influence cyanobacteria, especially their photosynthetic apparatus. Thylakoid membranes are known to be altered by far-red light photoacclimation (FaRLiP). However, the role of fatty acid synthesis and desaturation during FarLip is little studied. This study examines the gene expression of 33 fatty acid-related genes when the cyanobacterium Chlorogloeopsis fritschii PCC 6912 is grown under far-red light compared to white light using RNA-seq data from the NCBI database. The transcriptomic analysis, encompassing a bioinformatic pipeline to process and quantify transcripts, found that only two genes of the 33 examined genes were differentially expressed. The two genes were down-regulated and encoded for a fatty acid desaturase (fad) and a pfaD/polyketide biosynthesis protein. The results suggest that fatty acid desaturation decreases in favour of saturated fatty acids. Furthermore, the identification of a pfa gene cluster may highlight a secondary path of polyunsaturated fatty acids within heterocyst cyanobacteria. This study also highlights the need for greater biological replicates within RNA-seq experiments and increased completeness in genome annotations in biotechnological databases.
published_date 2023-05-10T16:05:11Z
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score 11.037056

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