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The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique

April Rees Orcid Logo, Zoe Edwards-I-Coll, Oliver Richards Orcid Logo, Molly E Raikes Orcid Logo, Roberto Angelini Orcid Logo, Cathy Thornton Orcid Logo

Molecular Omics, Volume: 19, Issue: 4, Pages: 340 - 350

Swansea University Authors: April Rees Orcid Logo, Oliver Richards Orcid Logo, Roberto Angelini Orcid Logo, Cathy Thornton Orcid Logo

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DOI (Published version): 10.1039/d2mo00294a

Abstract

The lipid environment changes throughout pregnancy both physiologically with emergent insulin resistance and pathologically e.g., gestational diabetes mellitus (GDM). Novel mass spectrometry (MS) techniques applied to minimally processed blood might lend themselves to monitoring changing lipid profi...

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Published in: Molecular Omics
ISSN: 2515-4184
Published: Royal Society of Chemistry (RSC) 2023
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URI: https://cronfa.swan.ac.uk/Record/cronfa63196
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Novel mass spectrometry (MS) techniques applied to minimally processed blood might lend themselves to monitoring changing lipid profiles to inform care decisions across pregnancy. In this study we use an intact-sandwich, MALDI-ToF MS method to identify phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) species and calculate their ratio as an indicator of inflammation. Plasma and sera were prepared from venous blood of non-pregnant women (aged 18–40) and pregnant women at 16 weeks, 28 weeks (including GDM-positive women), and 37+ weeks (term) of gestation alongside umbilical cord blood (UCB). Women with a normal menstrual cycle and age-matched men provided finger-prick derived capillary sera at 6 time-points over a month. Serum rather than plasma was preferable for PC/LPC measurement. As pregnancy progresses, an anti-inflammatory phenotype dominates the maternal circulation, evidenced by increasing PC/LPC ratio. In contrast, the PC/LPC ratio of UCB was aligned to that of non-pregnant donors. BMI had no significant effect on the PC/LPC ratio, but GDM-complicated pregnancies had significantly lower PC/LPC at 16 weeks of gestation. To further translate the use of the PC/LPC ratio clinically, the utility of finger-prick blood was evaluated; no significant difference between capillary versus venous serum was found and we revealed the PC/LPC ratio oscillates with the menstrual cycle. Overall, we show that the PC/LPC ratio can be measured simply in human serum and has the potential to be used as a time-efficient and less invasive biomarker of (mal)adaptative inflammation.</abstract><type>Journal Article</type><journal>Molecular Omics</journal><volume>19</volume><journalNumber>4</journalNumber><paginationStart>340</paginationStart><paginationEnd>350</paginationEnd><publisher>Royal Society of Chemistry (RSC)</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>2515-4184</issnElectronic><keywords>Lipidomics, pregnancy, novel mass spectrometry techniques, inflammation</keywords><publishedDay>8</publishedDay><publishedMonth>3</publishedMonth><publishedYear>2023</publishedYear><publishedDate>2023-03-08</publishedDate><doi>10.1039/d2mo00294a</doi><url>http://dx.doi.org/10.1039/d2mo00294a</url><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm>SU Library paid the OA fee (TA Institutional Deal)</apcterm><funders>This work was funded in part by Diabetes UK.</funders><projectreference/><lastEdited>2023-07-26T16:20:12.4770176</lastEdited><Created>2023-04-19T10:11:56.5412467</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>April</firstname><surname>Rees</surname><orcid>0000-0002-4408-634X</orcid><order>1</order></author><author><firstname>Zoe</firstname><surname>Edwards-I-Coll</surname><order>2</order></author><author><firstname>Oliver</firstname><surname>Richards</surname><orcid>0000-0002-5824-2745</orcid><order>3</order></author><author><firstname>Molly E</firstname><surname>Raikes</surname><orcid>0009-0009-0174-7687</orcid><order>4</order></author><author><firstname>Roberto</firstname><surname>Angelini</surname><orcid>0000-0001-5136-5921</orcid><order>5</order></author><author><firstname>Cathy</firstname><surname>Thornton</surname><orcid>0000-0002-5153-573X</orcid><order>6</order></author></authors><documents><document><filename>63196__27754__2a51b1310e9f4dd7b4180da9d2c3bea6.pdf</filename><originalFilename>63196.VOR.pdf</originalFilename><uploaded>2023-06-07T16:43:43.0387379</uploaded><type>Output</type><contentLength>1511921</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>Distributed under a Creative Commons Attribution 3.0 Unported (CC BY 3.0) Licence</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>https://creativecommons.org/licenses/by/3.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling v2 63196 2023-04-19 The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique ae088f7f8609d2b2ea4666f9b52b3c15 0000-0002-4408-634X April Rees April Rees true false 93c672d7c4bf8ebe19916d432f0ec7bb 0000-0002-5824-2745 Oliver Richards Oliver Richards true false 6405b7880498750d41c93c6ff89cff96 0000-0001-5136-5921 Roberto Angelini Roberto Angelini true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 2023-04-19 BMS The lipid environment changes throughout pregnancy both physiologically with emergent insulin resistance and pathologically e.g., gestational diabetes mellitus (GDM). Novel mass spectrometry (MS) techniques applied to minimally processed blood might lend themselves to monitoring changing lipid profiles to inform care decisions across pregnancy. In this study we use an intact-sandwich, MALDI-ToF MS method to identify phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) species and calculate their ratio as an indicator of inflammation. Plasma and sera were prepared from venous blood of non-pregnant women (aged 18–40) and pregnant women at 16 weeks, 28 weeks (including GDM-positive women), and 37+ weeks (term) of gestation alongside umbilical cord blood (UCB). Women with a normal menstrual cycle and age-matched men provided finger-prick derived capillary sera at 6 time-points over a month. Serum rather than plasma was preferable for PC/LPC measurement. As pregnancy progresses, an anti-inflammatory phenotype dominates the maternal circulation, evidenced by increasing PC/LPC ratio. In contrast, the PC/LPC ratio of UCB was aligned to that of non-pregnant donors. BMI had no significant effect on the PC/LPC ratio, but GDM-complicated pregnancies had significantly lower PC/LPC at 16 weeks of gestation. To further translate the use of the PC/LPC ratio clinically, the utility of finger-prick blood was evaluated; no significant difference between capillary versus venous serum was found and we revealed the PC/LPC ratio oscillates with the menstrual cycle. Overall, we show that the PC/LPC ratio can be measured simply in human serum and has the potential to be used as a time-efficient and less invasive biomarker of (mal)adaptative inflammation. Journal Article Molecular Omics 19 4 340 350 Royal Society of Chemistry (RSC) 2515-4184 Lipidomics, pregnancy, novel mass spectrometry techniques, inflammation 8 3 2023 2023-03-08 10.1039/d2mo00294a http://dx.doi.org/10.1039/d2mo00294a COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University SU Library paid the OA fee (TA Institutional Deal) This work was funded in part by Diabetes UK. 2023-07-26T16:20:12.4770176 2023-04-19T10:11:56.5412467 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine April Rees 0000-0002-4408-634X 1 Zoe Edwards-I-Coll 2 Oliver Richards 0000-0002-5824-2745 3 Molly E Raikes 0009-0009-0174-7687 4 Roberto Angelini 0000-0001-5136-5921 5 Cathy Thornton 0000-0002-5153-573X 6 63196__27754__2a51b1310e9f4dd7b4180da9d2c3bea6.pdf 63196.VOR.pdf 2023-06-07T16:43:43.0387379 Output 1511921 application/pdf Version of Record true Distributed under a Creative Commons Attribution 3.0 Unported (CC BY 3.0) Licence true eng https://creativecommons.org/licenses/by/3.0/
title The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique
spellingShingle The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique
April Rees
Oliver Richards
Roberto Angelini
Cathy Thornton
title_short The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique
title_full The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique
title_fullStr The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique
title_full_unstemmed The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique
title_sort The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique
author_id_str_mv ae088f7f8609d2b2ea4666f9b52b3c15
93c672d7c4bf8ebe19916d432f0ec7bb
6405b7880498750d41c93c6ff89cff96
c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv ae088f7f8609d2b2ea4666f9b52b3c15_***_April Rees
93c672d7c4bf8ebe19916d432f0ec7bb_***_Oliver Richards
6405b7880498750d41c93c6ff89cff96_***_Roberto Angelini
c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton
author April Rees
Oliver Richards
Roberto Angelini
Cathy Thornton
author2 April Rees
Zoe Edwards-I-Coll
Oliver Richards
Molly E Raikes
Roberto Angelini
Cathy Thornton
format Journal article
container_title Molecular Omics
container_volume 19
container_issue 4
container_start_page 340
publishDate 2023
institution Swansea University
issn 2515-4184
doi_str_mv 10.1039/d2mo00294a
publisher Royal Society of Chemistry (RSC)
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url http://dx.doi.org/10.1039/d2mo00294a
document_store_str 1
active_str 0
description The lipid environment changes throughout pregnancy both physiologically with emergent insulin resistance and pathologically e.g., gestational diabetes mellitus (GDM). Novel mass spectrometry (MS) techniques applied to minimally processed blood might lend themselves to monitoring changing lipid profiles to inform care decisions across pregnancy. In this study we use an intact-sandwich, MALDI-ToF MS method to identify phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) species and calculate their ratio as an indicator of inflammation. Plasma and sera were prepared from venous blood of non-pregnant women (aged 18–40) and pregnant women at 16 weeks, 28 weeks (including GDM-positive women), and 37+ weeks (term) of gestation alongside umbilical cord blood (UCB). Women with a normal menstrual cycle and age-matched men provided finger-prick derived capillary sera at 6 time-points over a month. Serum rather than plasma was preferable for PC/LPC measurement. As pregnancy progresses, an anti-inflammatory phenotype dominates the maternal circulation, evidenced by increasing PC/LPC ratio. In contrast, the PC/LPC ratio of UCB was aligned to that of non-pregnant donors. BMI had no significant effect on the PC/LPC ratio, but GDM-complicated pregnancies had significantly lower PC/LPC at 16 weeks of gestation. To further translate the use of the PC/LPC ratio clinically, the utility of finger-prick blood was evaluated; no significant difference between capillary versus venous serum was found and we revealed the PC/LPC ratio oscillates with the menstrual cycle. Overall, we show that the PC/LPC ratio can be measured simply in human serum and has the potential to be used as a time-efficient and less invasive biomarker of (mal)adaptative inflammation.
published_date 2023-03-08T16:19:27Z
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