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The sexual spore pigment asperthecin is required for normal ascospore production and protection from UV light in Aspergillus nidulans

Jonathan M Palmer, Philipp Wiemann, Claudio Greco Orcid Logo, Yi Ming Chiang, Clay C C Wang, Daniel L Lindner, Nancy P Keller

Journal of Industrial Microbiology and Biotechnology, Volume: 48, Issue: 9-10

Swansea University Author: Claudio Greco Orcid Logo

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DOI (Published version): 10.1093/jimb/kuab055

Abstract

Many fungi develop both asexual and sexual spores that serve as propagules for dissemination and/or recombination of genetic traits. Asexual spores are often heavily pigmented and this pigmentation provides protection from UV light. However, little is known about any purpose pigmentation that may se...

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Published in: Journal of Industrial Microbiology and Biotechnology
ISSN: 1367-5435 1476-5535
Published: Oxford University Press (OUP) 2021
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URI: https://cronfa.swan.ac.uk/Record/cronfa61513
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last_indexed 2023-01-13T19:22:18Z
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spelling 2022-10-20T13:44:47.6441511 v2 61513 2022-10-10 The sexual spore pigment asperthecin is required for normal ascospore production and protection from UV light in Aspergillus nidulans cacac6459bd7cf4a241f63661006036f 0000-0003-3067-0999 Claudio Greco Claudio Greco true false 2022-10-10 BGPS Many fungi develop both asexual and sexual spores that serve as propagules for dissemination and/or recombination of genetic traits. Asexual spores are often heavily pigmented and this pigmentation provides protection from UV light. However, little is known about any purpose pigmentation that may serve for sexual spores. The model Ascomycete Aspergillus nidulans produces both green pigmented asexual spores (conidia) and red pigmented sexual spores (ascospores). Here we find that the previously characterized red pigment, asperthecin, is the A. nidulans ascospore pigment. The asperthecin biosynthetic gene cluster is composed of three genes: aptA, aptB, and aptC, where deletion of either aptA (encoding a polyketide synthase) or aptB (encoding a thioesterase) yields small, mishappen hyaline ascospores; while deletion of aptC (encoding a monooxygenase) yields morphologically normal but purple ascospores. ∆aptA and ∆aptB but not ∆aptC or wild type ascospores are extremely sensitive to UV light. We find that two historical ascospore color mutants, clA6 and clB1, possess mutations in aptA and aptB sequences, respectively. Journal Article Journal of Industrial Microbiology and Biotechnology 48 9-10 Oxford University Press (OUP) 1367-5435 1476-5535 Polyketide, UV protection, Ascospore, Cleistothecia, Fungi 23 12 2021 2021-12-23 10.1093/jimb/kuab055 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University This work was supported in part by National Institutes of Health under grant 2R01GM112739-05A1 to N.P.K and by USDA Forest Service, Northern Research Station. 2022-10-20T13:44:47.6441511 2022-10-10T17:23:19.1892825 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Jonathan M Palmer 1 Philipp Wiemann 2 Claudio Greco 0000-0003-3067-0999 3 Yi Ming Chiang 4 Clay C C Wang 5 Daniel L Lindner 6 Nancy P Keller 7 61513__25525__7f91b71bd7b04b3d91c546ed3366c5a6.pdf 61513_VoR.pdf 2022-10-20T13:43:39.9575011 Output 1452926 application/pdf Version of Record true © The Author(s) 2021. This is an Open Access article distributed under the terms of the Creative Commons Attribution License true eng http://creativecommons.org/licenses/by/4.0/
title The sexual spore pigment asperthecin is required for normal ascospore production and protection from UV light in Aspergillus nidulans
spellingShingle The sexual spore pigment asperthecin is required for normal ascospore production and protection from UV light in Aspergillus nidulans
Claudio Greco
title_short The sexual spore pigment asperthecin is required for normal ascospore production and protection from UV light in Aspergillus nidulans
title_full The sexual spore pigment asperthecin is required for normal ascospore production and protection from UV light in Aspergillus nidulans
title_fullStr The sexual spore pigment asperthecin is required for normal ascospore production and protection from UV light in Aspergillus nidulans
title_full_unstemmed The sexual spore pigment asperthecin is required for normal ascospore production and protection from UV light in Aspergillus nidulans
title_sort The sexual spore pigment asperthecin is required for normal ascospore production and protection from UV light in Aspergillus nidulans
author_id_str_mv cacac6459bd7cf4a241f63661006036f
author_id_fullname_str_mv cacac6459bd7cf4a241f63661006036f_***_Claudio Greco
author Claudio Greco
author2 Jonathan M Palmer
Philipp Wiemann
Claudio Greco
Yi Ming Chiang
Clay C C Wang
Daniel L Lindner
Nancy P Keller
format Journal article
container_title Journal of Industrial Microbiology and Biotechnology
container_volume 48
container_issue 9-10
publishDate 2021
institution Swansea University
issn 1367-5435
1476-5535
doi_str_mv 10.1093/jimb/kuab055
publisher Oxford University Press (OUP)
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
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description Many fungi develop both asexual and sexual spores that serve as propagules for dissemination and/or recombination of genetic traits. Asexual spores are often heavily pigmented and this pigmentation provides protection from UV light. However, little is known about any purpose pigmentation that may serve for sexual spores. The model Ascomycete Aspergillus nidulans produces both green pigmented asexual spores (conidia) and red pigmented sexual spores (ascospores). Here we find that the previously characterized red pigment, asperthecin, is the A. nidulans ascospore pigment. The asperthecin biosynthetic gene cluster is composed of three genes: aptA, aptB, and aptC, where deletion of either aptA (encoding a polyketide synthase) or aptB (encoding a thioesterase) yields small, mishappen hyaline ascospores; while deletion of aptC (encoding a monooxygenase) yields morphologically normal but purple ascospores. ∆aptA and ∆aptB but not ∆aptC or wild type ascospores are extremely sensitive to UV light. We find that two historical ascospore color mutants, clA6 and clB1, possess mutations in aptA and aptB sequences, respectively.
published_date 2021-12-23T05:39:33Z
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