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Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.

Paul Dyson Orcid Logo, Miranda Walker (neeWhitten) Orcid Logo, Awawing Anjwengwo Andongma, Marcela Figueiredo Orcid Logo

Methods in molecular biology, Volume: 2360, Pages: 295 - 306

Swansea University Authors: Paul Dyson Orcid Logo, Miranda Walker (neeWhitten) Orcid Logo, Awawing Anjwengwo Andongma, Marcela Figueiredo Orcid Logo

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DOI (Published version): 10.1007/978-1-0716-1633-8_21

Abstract

RNA interference (RNAi) has emerged as a widely used approach for reverse genetic analysis in eukaryotes. In insects, RNAi also has an application in the control of insect pests. Several methods have been developed for delivery of interfering RNA in insects, with varying outcomes for different speci...

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Published in: Methods in molecular biology
ISBN: 978-1-0716-1632-1 978-1-0716-1633-8
Published: Protocol 2021
URI: https://cronfa.swan.ac.uk/Record/cronfa60220
first_indexed 2022-07-07T12:19:25Z
last_indexed 2023-01-13T19:20:11Z
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spelling 2022-07-07T13:19:47.3772252 v2 60220 2022-06-14 Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects. 300e3f46b70ae83f563b24f41d00cd17 0000-0002-0558-2666 Paul Dyson Paul Dyson true false 83389c57d000a704fae36fda87d7ca76 0000-0003-1930-5042 Miranda Walker (neeWhitten) Miranda Walker (neeWhitten) true false 6d83c753a85242a9fb9a366ab5636d69 Awawing Anjwengwo Andongma Awawing Anjwengwo Andongma true false b6d8f11df85fce6d8bd702f565908a79 0000-0001-6374-358X Marcela Figueiredo Marcela Figueiredo true false 2022-06-14 MEDS RNA interference (RNAi) has emerged as a widely used approach for reverse genetic analysis in eukaryotes. In insects, RNAi also has an application in the control of insect pests. Several methods have been developed for delivery of interfering RNA in insects, with varying outcomes for different species. Here we describe how a bacterial symbiont can be exploited for continuous synthesis of interfering double-stranded RNA (dsRNA) in its insect host. This approach, termed symbiont-mediated RNAi (SMR), can overcome problems associated with instability of dietary dsRNA due to action of salivary or foregut nucleases. As insects do not possess RNA-dependent RNA polymerase activity that can amplify and extend RNAi in other organisms, SMR also offers the possibility of long-term systemic RNAi not afforded by single applications of dsRNA to insects by other delivery methods. Here, we describe how SMR can be applied in a globally distributed agricultural pest species, western flower thrips (Frankliniella occidentalis). Other Methods in molecular biology 2360 295 306 Protocol 978-1-0716-1632-1 978-1-0716-1633-8 9 9 2021 2021-09-09 10.1007/978-1-0716-1633-8_21 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University Not Required This work was funded by a Grand Challenges Explorations grant from the Bill and Melinda Gates Foundation (OPP1068514), and a grant from the Biotechnology and Biological Sciences Research Council, UK (BB/R006418/1). 2022-07-07T13:19:47.3772252 2022-06-14T12:40:52.6208416 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Paul Dyson 0000-0002-0558-2666 1 Miranda Walker (neeWhitten) 0000-0003-1930-5042 2 Awawing Anjwengwo Andongma 3 Marcela Figueiredo 0000-0001-6374-358X 4
title Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.
spellingShingle Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.
Paul Dyson
Miranda Walker (neeWhitten)
Awawing Anjwengwo Andongma
Marcela Figueiredo
title_short Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.
title_full Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.
title_fullStr Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.
title_full_unstemmed Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.
title_sort Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.
author_id_str_mv 300e3f46b70ae83f563b24f41d00cd17
83389c57d000a704fae36fda87d7ca76
6d83c753a85242a9fb9a366ab5636d69
b6d8f11df85fce6d8bd702f565908a79
author_id_fullname_str_mv 300e3f46b70ae83f563b24f41d00cd17_***_Paul Dyson
83389c57d000a704fae36fda87d7ca76_***_Miranda Walker (neeWhitten)
6d83c753a85242a9fb9a366ab5636d69_***_Awawing Anjwengwo Andongma
b6d8f11df85fce6d8bd702f565908a79_***_Marcela Figueiredo
author Paul Dyson
Miranda Walker (neeWhitten)
Awawing Anjwengwo Andongma
Marcela Figueiredo
author2 Paul Dyson
Miranda Walker (neeWhitten)
Awawing Anjwengwo Andongma
Marcela Figueiredo
format Other
container_title Methods in molecular biology
container_volume 2360
container_start_page 295
publishDate 2021
institution Swansea University
isbn 978-1-0716-1632-1
978-1-0716-1633-8
doi_str_mv 10.1007/978-1-0716-1633-8_21
publisher Protocol
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description RNA interference (RNAi) has emerged as a widely used approach for reverse genetic analysis in eukaryotes. In insects, RNAi also has an application in the control of insect pests. Several methods have been developed for delivery of interfering RNA in insects, with varying outcomes for different species. Here we describe how a bacterial symbiont can be exploited for continuous synthesis of interfering double-stranded RNA (dsRNA) in its insect host. This approach, termed symbiont-mediated RNAi (SMR), can overcome problems associated with instability of dietary dsRNA due to action of salivary or foregut nucleases. As insects do not possess RNA-dependent RNA polymerase activity that can amplify and extend RNAi in other organisms, SMR also offers the possibility of long-term systemic RNAi not afforded by single applications of dsRNA to insects by other delivery methods. Here, we describe how SMR can be applied in a globally distributed agricultural pest species, western flower thrips (Frankliniella occidentalis).
published_date 2021-09-09T20:12:21Z
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