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Odd chain fatty acid metabolism in mice after a high fat diet

Isaac Ampong, O. John Ikwuobe, James E.P. Brown, Clifford J. Bailey, Dan Gao, Jorge Gutierrez-Merino, Helen Griffiths

The International Journal of Biochemistry & Cell Biology, Volume: 143, Start page: 106135

Swansea University Author: Helen Griffiths

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DOI (Published version): 10.1016/j.biocel.2021.106135

Abstract

Epidemiological studies show that higher circulating levels of odd chain saturated fatty acids (FA: C15:0 and C17:0) are associated with lower risk of metabolic disease. These odd chain saturated fatty acids (OCSFA) are produced by α-oxidation in peroxisomes, de novo lipogenesis, from the diet and b...

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Published in: The International Journal of Biochemistry & Cell Biology
ISSN: 1357-2725
Published: Elsevier BV 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa59403
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spelling 2022-08-08T15:19:31.0612510 v2 59403 2022-02-15 Odd chain fatty acid metabolism in mice after a high fat diet 0366ea9a689b222146b7d63c6baf8427 Helen Griffiths Helen Griffiths true false 2022-02-15 SMT Epidemiological studies show that higher circulating levels of odd chain saturated fatty acids (FA: C15:0 and C17:0) are associated with lower risk of metabolic disease. These odd chain saturated fatty acids (OCSFA) are produced by α-oxidation in peroxisomes, de novo lipogenesis, from the diet and by gut microbiota. Although present at low concentrations, they are of interest as potential targets to reduce metabolic disease risk. To determine whether OCSFA are affected by obesogenic diets, we have investigated whether high dietary fat intake affects the frequency of OCSFA-producing gut microbiota, liver lipid metabolism genes and circulating OCSFA. FA concentrations were determined in liver and serum from pathogen-free SPF C57BL/6 J mice fed either standard chow or a high fat diet (HFD; 60% calories as fat) for four and twelve weeks. Post-mortem mouse livers were analysed histologically for fat deposition by gas chromatography–mass spectrometry for FA composition and by qPCR for the lipid metabolic genes fatty acid desaturase 2 (FADS2), stearoyl CoA desaturase 1 (SCD1), elongation of long-chain fatty acids family member 6 (ELOVL6) and 2-hydroxyacyl-CoA lyase 1 (HACL). Gut microbiota in faecal pellets from the ileum were analysed by 16S RNA sequencing. A significant depletion of serum and liver C15:0 (>50%; P < 0.05) and liver C17:0 (>35%; P < 0.05) was observed in HFD-fed SPF mice in parallel with hepatic fat accumulation after four weeks. In addition, liver gene expression (HACL1, ELOVL6, SCD1 and FADS2) was lower (>50%; P < 0.05) and the relative abundance of beneficial C3:0-producing gut bacteria such as Akkermansia, Lactobacillus, Bifidobacterium was lower after HFD in SPF mice. In summary, high dietary fat intake reduces serum and liver OCSFA, OCSFA-producing gut microbiota and is associated with impaired liver lipid metabolism. Further studies are required to identify whether there is any beneficial effect of OCSFA and C3:0-producing gut bacteria to counter metabolic disease. Journal Article The International Journal of Biochemistry & Cell Biology 143 106135 Elsevier BV 1357-2725 Free fatty acids; Gut microbiota; Odd-chain saturated fatty acids; Steatosis; Liver 1 2 2022 2022-02-01 10.1016/j.biocel.2021.106135 COLLEGE NANME Senior Leadership Team COLLEGE CODE SMT Swansea University 2022-08-08T15:19:31.0612510 2022-02-15T15:01:23.2346084 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Isaac Ampong 1 O. John Ikwuobe 2 James E.P. Brown 3 Clifford J. Bailey 4 Dan Gao 5 Jorge Gutierrez-Merino 6 Helen Griffiths 7 59403__22496__d9b89310c60142d7818444795a3b2b6f.pdf 59403.pdf 2022-03-02T14:31:38.1725504 Output 3199977 application/pdf Version of Record true © 2021 The Author(s). This is an open access article under the CC BY license true eng http://creativecommons.org/licenses/by/4.0/
title Odd chain fatty acid metabolism in mice after a high fat diet
spellingShingle Odd chain fatty acid metabolism in mice after a high fat diet
Helen Griffiths
title_short Odd chain fatty acid metabolism in mice after a high fat diet
title_full Odd chain fatty acid metabolism in mice after a high fat diet
title_fullStr Odd chain fatty acid metabolism in mice after a high fat diet
title_full_unstemmed Odd chain fatty acid metabolism in mice after a high fat diet
title_sort Odd chain fatty acid metabolism in mice after a high fat diet
author_id_str_mv 0366ea9a689b222146b7d63c6baf8427
author_id_fullname_str_mv 0366ea9a689b222146b7d63c6baf8427_***_Helen Griffiths
author Helen Griffiths
author2 Isaac Ampong
O. John Ikwuobe
James E.P. Brown
Clifford J. Bailey
Dan Gao
Jorge Gutierrez-Merino
Helen Griffiths
format Journal article
container_title The International Journal of Biochemistry & Cell Biology
container_volume 143
container_start_page 106135
publishDate 2022
institution Swansea University
issn 1357-2725
doi_str_mv 10.1016/j.biocel.2021.106135
publisher Elsevier BV
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
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description Epidemiological studies show that higher circulating levels of odd chain saturated fatty acids (FA: C15:0 and C17:0) are associated with lower risk of metabolic disease. These odd chain saturated fatty acids (OCSFA) are produced by α-oxidation in peroxisomes, de novo lipogenesis, from the diet and by gut microbiota. Although present at low concentrations, they are of interest as potential targets to reduce metabolic disease risk. To determine whether OCSFA are affected by obesogenic diets, we have investigated whether high dietary fat intake affects the frequency of OCSFA-producing gut microbiota, liver lipid metabolism genes and circulating OCSFA. FA concentrations were determined in liver and serum from pathogen-free SPF C57BL/6 J mice fed either standard chow or a high fat diet (HFD; 60% calories as fat) for four and twelve weeks. Post-mortem mouse livers were analysed histologically for fat deposition by gas chromatography–mass spectrometry for FA composition and by qPCR for the lipid metabolic genes fatty acid desaturase 2 (FADS2), stearoyl CoA desaturase 1 (SCD1), elongation of long-chain fatty acids family member 6 (ELOVL6) and 2-hydroxyacyl-CoA lyase 1 (HACL). Gut microbiota in faecal pellets from the ileum were analysed by 16S RNA sequencing. A significant depletion of serum and liver C15:0 (>50%; P < 0.05) and liver C17:0 (>35%; P < 0.05) was observed in HFD-fed SPF mice in parallel with hepatic fat accumulation after four weeks. In addition, liver gene expression (HACL1, ELOVL6, SCD1 and FADS2) was lower (>50%; P < 0.05) and the relative abundance of beneficial C3:0-producing gut bacteria such as Akkermansia, Lactobacillus, Bifidobacterium was lower after HFD in SPF mice. In summary, high dietary fat intake reduces serum and liver OCSFA, OCSFA-producing gut microbiota and is associated with impaired liver lipid metabolism. Further studies are required to identify whether there is any beneficial effect of OCSFA and C3:0-producing gut bacteria to counter metabolic disease.
published_date 2022-02-01T04:16:41Z
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