No Cover Image

Journal article 1237 views

Fabrication of Micro-Nano Bioactive Glass Scaffold Incorporated with Siglec-15 for Bone Repair and Postoperative Treatment of Osteosarcoma

Xiaorong Li, Caiping Yan, Dengyuan Wang, Hong Lu, Zhidao Xia

Science of Advanced Materials, Volume: 13, Issue: 8, Pages: 1445 - 1451

Swansea University Authors: Xiaorong Li, Hong Lu, Zhidao Xia

Full text not available from this repository: check for access using links below.

Check full text

DOI (Published version): 10.1166/sam.2021.4000

Abstract

This study aimed to fabricate micro-nano bioactive glass (MNBG) scaffolds loaded with chemotherapeutics and siglec-15 monoclonal antibody with bone repair capability and high-active drug loading capability for postoperative treatment of osteosarcoma. Bioactive glass (BG) particles were incorporated...

Full description

Published in: Science of Advanced Materials
ISSN: 1947-2935
Published: American Scientific Publishers 2021
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa58672
first_indexed 2021-11-15T14:29:32Z
last_indexed 2021-12-08T04:18:53Z
id cronfa58672
recordtype SURis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2021-12-07T16:08:41.1014276</datestamp><bib-version>v2</bib-version><id>58672</id><entry>2021-11-15</entry><title>Fabrication of Micro-Nano Bioactive Glass Scaffold Incorporated with Siglec-15 for Bone Repair and Postoperative Treatment of Osteosarcoma</title><swanseaauthors><author><sid>583f9bf2d663c1feb79b086d807a3bec</sid><firstname>Xiaorong</firstname><surname>Li</surname><name>Xiaorong Li</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>6aba1fd9584e82c6909e7f37ecf6ccc0</sid><firstname>Hong</firstname><surname>Lu</surname><name>Hong Lu</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>c9307abfed1b43987a19da0c0e30d7a4</sid><firstname>Zhidao</firstname><surname>Xia</surname><name>Zhidao Xia</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2021-11-15</date><deptcode>ACEM</deptcode><abstract>This study aimed to fabricate micro-nano bioactive glass (MNBG) scaffolds loaded with chemotherapeutics and siglec-15 monoclonal antibody with bone repair capability and high-active drug loading capability for postoperative treatment of osteosarcoma. Bioactive glass (BG) particles were incorporated with siglec-15 mAb and gemcitabine through mesopores and calcium ions on the surface. Dendritic cells (DCs) were treated with siglec-loaded BGs and gemcitabine. RT-qPCR analysis was conducted to detect DJ-1 and PTEN mRNA levels, CCK-8 technology to detect cell activity, and flow cytometry to detect cell apoptosis. Rats were administrated with siglec-15-MNBG composite scaffolds with/without gemcitabine. 3D printing was used to determine adhesion strength of each group. Administration of MNBG scaffold decreased the expression of PTEN and up-regulated expression of DJ-1 when inducing cell apoptosis. Combined treatment with gemcitabine augmented adhesion of material and enhanced phosphorylation activity of p-AKT, mitigating the inhibitory effect of scaffold loaded with siglec-15 on p-AKT protein expression. Collectively, MNBG scaffold loaded with siglec-15 might promote bone regeneration and incorporate with chemotherapeutic drugs to suppress tumor development and promote apoptosis through PTEN/PI3 K pathway. These findings provide a novel insight into postoperative treatment of osteosarcoma and help development of tumor immunotherapy/bone repair integrated materials.</abstract><type>Journal Article</type><journal>Science of Advanced Materials</journal><volume>13</volume><journalNumber>8</journalNumber><paginationStart>1445</paginationStart><paginationEnd>1451</paginationEnd><publisher>American Scientific Publishers</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>1947-2935</issnPrint><issnElectronic/><keywords>Apoptosis; Bioactive Glass; Osteosarcoma; PTEN/PI3K/AKT; Scaffold; Siglec-15</keywords><publishedDay>1</publishedDay><publishedMonth>8</publishedMonth><publishedYear>2021</publishedYear><publishedDate>2021-08-01</publishedDate><doi>10.1166/sam.2021.4000</doi><url/><notes/><college>COLLEGE NANME</college><department>Aerospace, Civil, Electrical, and Mechanical Engineering</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>ACEM</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2021-12-07T16:08:41.1014276</lastEdited><Created>2021-11-15T14:25:10.2354740</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Xiaorong</firstname><surname>Li</surname><order>1</order></author><author><firstname>Caiping</firstname><surname>Yan</surname><order>2</order></author><author><firstname>Dengyuan</firstname><surname>Wang</surname><order>3</order></author><author><firstname>Hong</firstname><surname>Lu</surname><order>4</order></author><author><firstname>Zhidao</firstname><surname>Xia</surname><order>5</order></author></authors><documents/><OutputDurs/></rfc1807>
spelling 2021-12-07T16:08:41.1014276 v2 58672 2021-11-15 Fabrication of Micro-Nano Bioactive Glass Scaffold Incorporated with Siglec-15 for Bone Repair and Postoperative Treatment of Osteosarcoma 583f9bf2d663c1feb79b086d807a3bec Xiaorong Li Xiaorong Li true false 6aba1fd9584e82c6909e7f37ecf6ccc0 Hong Lu Hong Lu true false c9307abfed1b43987a19da0c0e30d7a4 Zhidao Xia Zhidao Xia true false 2021-11-15 ACEM This study aimed to fabricate micro-nano bioactive glass (MNBG) scaffolds loaded with chemotherapeutics and siglec-15 monoclonal antibody with bone repair capability and high-active drug loading capability for postoperative treatment of osteosarcoma. Bioactive glass (BG) particles were incorporated with siglec-15 mAb and gemcitabine through mesopores and calcium ions on the surface. Dendritic cells (DCs) were treated with siglec-loaded BGs and gemcitabine. RT-qPCR analysis was conducted to detect DJ-1 and PTEN mRNA levels, CCK-8 technology to detect cell activity, and flow cytometry to detect cell apoptosis. Rats were administrated with siglec-15-MNBG composite scaffolds with/without gemcitabine. 3D printing was used to determine adhesion strength of each group. Administration of MNBG scaffold decreased the expression of PTEN and up-regulated expression of DJ-1 when inducing cell apoptosis. Combined treatment with gemcitabine augmented adhesion of material and enhanced phosphorylation activity of p-AKT, mitigating the inhibitory effect of scaffold loaded with siglec-15 on p-AKT protein expression. Collectively, MNBG scaffold loaded with siglec-15 might promote bone regeneration and incorporate with chemotherapeutic drugs to suppress tumor development and promote apoptosis through PTEN/PI3 K pathway. These findings provide a novel insight into postoperative treatment of osteosarcoma and help development of tumor immunotherapy/bone repair integrated materials. Journal Article Science of Advanced Materials 13 8 1445 1451 American Scientific Publishers 1947-2935 Apoptosis; Bioactive Glass; Osteosarcoma; PTEN/PI3K/AKT; Scaffold; Siglec-15 1 8 2021 2021-08-01 10.1166/sam.2021.4000 COLLEGE NANME Aerospace, Civil, Electrical, and Mechanical Engineering COLLEGE CODE ACEM Swansea University 2021-12-07T16:08:41.1014276 2021-11-15T14:25:10.2354740 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Xiaorong Li 1 Caiping Yan 2 Dengyuan Wang 3 Hong Lu 4 Zhidao Xia 5
title Fabrication of Micro-Nano Bioactive Glass Scaffold Incorporated with Siglec-15 for Bone Repair and Postoperative Treatment of Osteosarcoma
spellingShingle Fabrication of Micro-Nano Bioactive Glass Scaffold Incorporated with Siglec-15 for Bone Repair and Postoperative Treatment of Osteosarcoma
Xiaorong Li
Hong Lu
Zhidao Xia
title_short Fabrication of Micro-Nano Bioactive Glass Scaffold Incorporated with Siglec-15 for Bone Repair and Postoperative Treatment of Osteosarcoma
title_full Fabrication of Micro-Nano Bioactive Glass Scaffold Incorporated with Siglec-15 for Bone Repair and Postoperative Treatment of Osteosarcoma
title_fullStr Fabrication of Micro-Nano Bioactive Glass Scaffold Incorporated with Siglec-15 for Bone Repair and Postoperative Treatment of Osteosarcoma
title_full_unstemmed Fabrication of Micro-Nano Bioactive Glass Scaffold Incorporated with Siglec-15 for Bone Repair and Postoperative Treatment of Osteosarcoma
title_sort Fabrication of Micro-Nano Bioactive Glass Scaffold Incorporated with Siglec-15 for Bone Repair and Postoperative Treatment of Osteosarcoma
author_id_str_mv 583f9bf2d663c1feb79b086d807a3bec
6aba1fd9584e82c6909e7f37ecf6ccc0
c9307abfed1b43987a19da0c0e30d7a4
author_id_fullname_str_mv 583f9bf2d663c1feb79b086d807a3bec_***_Xiaorong Li
6aba1fd9584e82c6909e7f37ecf6ccc0_***_Hong Lu
c9307abfed1b43987a19da0c0e30d7a4_***_Zhidao Xia
author Xiaorong Li
Hong Lu
Zhidao Xia
author2 Xiaorong Li
Caiping Yan
Dengyuan Wang
Hong Lu
Zhidao Xia
format Journal article
container_title Science of Advanced Materials
container_volume 13
container_issue 8
container_start_page 1445
publishDate 2021
institution Swansea University
issn 1947-2935
doi_str_mv 10.1166/sam.2021.4000
publisher American Scientific Publishers
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
active_str 0
description This study aimed to fabricate micro-nano bioactive glass (MNBG) scaffolds loaded with chemotherapeutics and siglec-15 monoclonal antibody with bone repair capability and high-active drug loading capability for postoperative treatment of osteosarcoma. Bioactive glass (BG) particles were incorporated with siglec-15 mAb and gemcitabine through mesopores and calcium ions on the surface. Dendritic cells (DCs) were treated with siglec-loaded BGs and gemcitabine. RT-qPCR analysis was conducted to detect DJ-1 and PTEN mRNA levels, CCK-8 technology to detect cell activity, and flow cytometry to detect cell apoptosis. Rats were administrated with siglec-15-MNBG composite scaffolds with/without gemcitabine. 3D printing was used to determine adhesion strength of each group. Administration of MNBG scaffold decreased the expression of PTEN and up-regulated expression of DJ-1 when inducing cell apoptosis. Combined treatment with gemcitabine augmented adhesion of material and enhanced phosphorylation activity of p-AKT, mitigating the inhibitory effect of scaffold loaded with siglec-15 on p-AKT protein expression. Collectively, MNBG scaffold loaded with siglec-15 might promote bone regeneration and incorporate with chemotherapeutic drugs to suppress tumor development and promote apoptosis through PTEN/PI3 K pathway. These findings provide a novel insight into postoperative treatment of osteosarcoma and help development of tumor immunotherapy/bone repair integrated materials.
published_date 2021-08-01T04:59:55Z
_version_ 1851096087027253248
score 11.089407

Similar Items