Journal article 778 views
Fabrication of Micro-Nano Bioactive Glass Scaffold Incorporated with Siglec-15 for Bone Repair and Postoperative Treatment of Osteosarcoma
Science of Advanced Materials, Volume: 13, Issue: 8, Pages: 1445 - 1451
Swansea University Authors:
Xiaorong Li, Hong Lu, Zhidao Xia
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DOI (Published version): 10.1166/sam.2021.4000
Abstract
This study aimed to fabricate micro-nano bioactive glass (MNBG) scaffolds loaded with chemotherapeutics and siglec-15 monoclonal antibody with bone repair capability and high-active drug loading capability for postoperative treatment of osteosarcoma. Bioactive glass (BG) particles were incorporated...
| Published in: | Science of Advanced Materials |
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| ISSN: | 1947-2935 |
| Published: |
American Scientific Publishers
2021
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa58672 |
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| Abstract: |
This study aimed to fabricate micro-nano bioactive glass (MNBG) scaffolds loaded with chemotherapeutics and siglec-15 monoclonal antibody with bone repair capability and high-active drug loading capability for postoperative treatment of osteosarcoma. Bioactive glass (BG) particles were incorporated with siglec-15 mAb and gemcitabine through mesopores and calcium ions on the surface. Dendritic cells (DCs) were treated with siglec-loaded BGs and gemcitabine. RT-qPCR analysis was conducted to detect DJ-1 and PTEN mRNA levels, CCK-8 technology to detect cell activity, and flow cytometry to detect cell apoptosis. Rats were administrated with siglec-15-MNBG composite scaffolds with/without gemcitabine. 3D printing was used to determine adhesion strength of each group. Administration of MNBG scaffold decreased the expression of PTEN and up-regulated expression of DJ-1 when inducing cell apoptosis. Combined treatment with gemcitabine augmented adhesion of material and enhanced phosphorylation activity of p-AKT, mitigating the inhibitory effect of scaffold loaded with siglec-15 on p-AKT protein expression. Collectively, MNBG scaffold loaded with siglec-15 might promote bone regeneration and incorporate with chemotherapeutic drugs to suppress tumor development and promote apoptosis through PTEN/PI3 K pathway. These findings provide a novel insight into postoperative treatment of osteosarcoma and help development of tumor immunotherapy/bone repair integrated materials. |
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| Keywords: |
Apoptosis; Bioactive Glass; Osteosarcoma; PTEN/PI3K/AKT; Scaffold; Siglec-15 |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Issue: |
8 |
| Start Page: |
1445 |
| End Page: |
1451 |