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A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil

Rajesh Sreedharan Nair, Nashiru Billa, Andrew Morris Orcid Logo

Current Nutrition & Food Science, Volume: 17

Swansea University Author: Andrew Morris Orcid Logo

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DOI (Published version): 10.2174/1573401317666210803155717

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Background: Palm oil and its constituents have wide applications in food, cosmetics and pharmaceutical industries. The tocotrienol-rich fractions of crude palm oil have drawn greater research interest in recent years due to its potent health benefits. Therefore, reliable and validated analytical met...

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Published in: Current Nutrition & Food Science
ISSN: 1573-4013
Published: Bentham Science Publishers Ltd. 2021
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URI: https://cronfa.swan.ac.uk/Record/cronfa57832
first_indexed 2021-09-09T21:39:16Z
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spelling 2025-01-02T10:53:38.0833321 v2 57832 2021-09-09 A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil 3d7a7f540a5143114fcaf67e4c68d151 0000-0002-6315-7553 Andrew Morris Andrew Morris true false 2021-09-09 MEDS Background: Palm oil and its constituents have wide applications in food, cosmetics and pharmaceutical industries. The tocotrienol-rich fractions of crude palm oil have drawn greater research interest in recent years due to its potent health benefits. Therefore, reliable and validated analytical methods are essential for the quantification of tocotrienols.Objective: This study aimed to develop a simple and economical RP-HPLC method for the quantification of gamma-tocotrienol in tocotrienol rich fractions of crude palm oil.Methods: An Agilent HPLC system supplied with a Diode Array detector and an auto-injector system was used for the method development, and the wavelength was set 295 nm. A reversed-phase C18 column maintained at 30 °C using a mobile phase composition of methanol: water (95:05) at a flow rate of 1 mL/min was used for the analysis. The developed method was validated according to ICH guidelines.Results and discussion: A symmetrical peak of gamma-tocotrienol was observed at 8.7 minutes with minimal peak tailing (between 0.76 and 0.78), and an acceptable resolution above 2.0. Excellent linearity was evident with R2 values 0.9996 and 0.9991 for intra-day and inter-day respectively. The method demonstrated a high precision (%RSD values ≤ 5.8%) and accuracy (%RE<9.6%). The LOD and LOQ of gamma-tocotrienol were determined as 1.4 μg/mL and 4.2 μg/mL respectively. The system suitability studies indicate that the chromatographic parameters are well within the acceptable limit.Conclusion: In conclusion, the developed RP-HPLC method is rapid, precise, stable and economical for the quantification of gamma-tocotrienol. Journal Article Current Nutrition & Food Science 17 Bentham Science Publishers Ltd. 1573-4013 TRF, gamma-tocotrienol, RP-HPLC, validation, ICH, system suitability. 3 8 2021 2021-08-03 10.2174/1573401317666210803155717 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2025-01-02T10:53:38.0833321 2021-09-09T22:35:10.6206819 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Rajesh Sreedharan Nair 1 Nashiru Billa 2 Andrew Morris 0000-0002-6315-7553 3 57832__20840__0eab8d8eb2814e98a09fdc05c8682423.pdf 57832.pdf 2021-09-13T17:13:39.3246276 Output 530072 application/pdf Accepted Manuscript true 2022-08-03T00:00:00.0000000 true eng
title A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
spellingShingle A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
Andrew Morris
title_short A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
title_full A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
title_fullStr A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
title_full_unstemmed A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
title_sort A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
author_id_str_mv 3d7a7f540a5143114fcaf67e4c68d151
author_id_fullname_str_mv 3d7a7f540a5143114fcaf67e4c68d151_***_Andrew Morris
author Andrew Morris
author2 Rajesh Sreedharan Nair
Nashiru Billa
Andrew Morris
format Journal article
container_title Current Nutrition & Food Science
container_volume 17
publishDate 2021
institution Swansea University
issn 1573-4013
doi_str_mv 10.2174/1573401317666210803155717
publisher Bentham Science Publishers Ltd.
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description Background: Palm oil and its constituents have wide applications in food, cosmetics and pharmaceutical industries. The tocotrienol-rich fractions of crude palm oil have drawn greater research interest in recent years due to its potent health benefits. Therefore, reliable and validated analytical methods are essential for the quantification of tocotrienols.Objective: This study aimed to develop a simple and economical RP-HPLC method for the quantification of gamma-tocotrienol in tocotrienol rich fractions of crude palm oil.Methods: An Agilent HPLC system supplied with a Diode Array detector and an auto-injector system was used for the method development, and the wavelength was set 295 nm. A reversed-phase C18 column maintained at 30 °C using a mobile phase composition of methanol: water (95:05) at a flow rate of 1 mL/min was used for the analysis. The developed method was validated according to ICH guidelines.Results and discussion: A symmetrical peak of gamma-tocotrienol was observed at 8.7 minutes with minimal peak tailing (between 0.76 and 0.78), and an acceptable resolution above 2.0. Excellent linearity was evident with R2 values 0.9996 and 0.9991 for intra-day and inter-day respectively. The method demonstrated a high precision (%RSD values ≤ 5.8%) and accuracy (%RE<9.6%). The LOD and LOQ of gamma-tocotrienol were determined as 1.4 μg/mL and 4.2 μg/mL respectively. The system suitability studies indicate that the chromatographic parameters are well within the acceptable limit.Conclusion: In conclusion, the developed RP-HPLC method is rapid, precise, stable and economical for the quantification of gamma-tocotrienol.
published_date 2021-08-03T08:04:37Z
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score 11.047501

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