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Naked-Eye Detection of Hepatitis B Surface Antigen Using Gold Nanoparticles Aggregation and Catalase-Functionalized Polystyrene Nanospheres

Rubing Chen, Yongqin Hu, Meizhu Chen, Jia An, Ying Lyu, Yufei Liu, Dongling Li

ACS Omega, Volume: 6, Issue: 14, Pages: 9828 - 9833

Swansea University Author: Yufei Liu

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DOI (Published version): 10.1021/acsomega.1c00507

Abstract

Developing rapid, efficient, highly sensitive, simple, stable, and low-cost virus marker detection products that are appropriate for basic facilities is of great importance in the early diagnosis and treatment of viruses. Naked-eye detection methods are especially important when medical testing faci...

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Published in: ACS Omega
ISSN: 2470-1343 2470-1343
Published: American Chemical Society (ACS) 2021
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URI: https://cronfa.swan.ac.uk/Record/cronfa57787
first_indexed 2021-09-07T09:43:07Z
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spelling 2021-09-07T10:56:20.3538890 v2 57787 2021-09-07 Naked-Eye Detection of Hepatitis B Surface Antigen Using Gold Nanoparticles Aggregation and Catalase-Functionalized Polystyrene Nanospheres b6e81312388169e1da3e505660e08f32 Yufei Liu Yufei Liu true false 2021-09-07 Developing rapid, efficient, highly sensitive, simple, stable, and low-cost virus marker detection products that are appropriate for basic facilities is of great importance in the early diagnosis and treatment of viruses. Naked-eye detection methods are especially important when medical testing facilities are limited. Polystyrene nanospheres (PSs) with catalytic and specific recognition functions were successfully developed by simultaneously modifying catalase and goat anti-hepatitis B surface antibodies on nanospheres. The modified PSs contributed significantly to the amplification of the signal. Via the specific antigen–antibody reaction, the bifunctional nanospheres could be captured on microplate and then catalyzed the decomposition of hydrogen peroxide to reduce chloroauric acid and synthesize gold nanoparticles (AuNPs). Due to the surface plasmon resonance of AuNPs, the solution color change could be observed with the naked eye and the limit of detection (LOD) was 0.1 ng/mL. Furthermore, the LOD observed with instrumentation was 0.01 ng/mL, which meant that a rapid, efficient, and highly sensitive method for the detection of hepatitis B surface antigens was successfully developed, and neither complex sample pretreatment nor expensive equipment was needed. Journal Article ACS Omega 6 14 9828 9833 American Chemical Society (ACS) 2470-1343 2470-1343 General Chemical Engineering, General Chemistry 13 4 2021 2021-04-13 10.1021/acsomega.1c00507 COLLEGE NANME COLLEGE CODE Swansea University Other National Natural Science Foundation of China Grant: 61927818 Identifier: FundRef 10.13039/501100001809 Fundamental Research Funds for the Central Universities of China Grant: 2019CDCGGD304 Grant: 2019CDQYGD020 Identifier: FundRef 10.13039/501100012226 National Key Research and Development Program of China Grant: 2016YFE0125200 2021-09-07T10:56:20.3538890 2021-09-07T10:38:00.6759131 Faculty of Science and Engineering School of Engineering and Applied Sciences - Uncategorised Rubing Chen 1 Yongqin Hu 2 Meizhu Chen 3 Jia An 4 Ying Lyu 5 Yufei Liu 6 Dongling Li 7 57787__20762__b876442d0c1242e382b2a33915bbf74c.pdf 57787.pdf 2021-09-07T10:43:27.9750898 Output 2924318 application/pdf Version of Record true © 2021 The Authors. Released under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License true eng https://creativecommons.org/licenses/by-nc-nd/4.0/
title Naked-Eye Detection of Hepatitis B Surface Antigen Using Gold Nanoparticles Aggregation and Catalase-Functionalized Polystyrene Nanospheres
spellingShingle Naked-Eye Detection of Hepatitis B Surface Antigen Using Gold Nanoparticles Aggregation and Catalase-Functionalized Polystyrene Nanospheres
Yufei Liu
title_short Naked-Eye Detection of Hepatitis B Surface Antigen Using Gold Nanoparticles Aggregation and Catalase-Functionalized Polystyrene Nanospheres
title_full Naked-Eye Detection of Hepatitis B Surface Antigen Using Gold Nanoparticles Aggregation and Catalase-Functionalized Polystyrene Nanospheres
title_fullStr Naked-Eye Detection of Hepatitis B Surface Antigen Using Gold Nanoparticles Aggregation and Catalase-Functionalized Polystyrene Nanospheres
title_full_unstemmed Naked-Eye Detection of Hepatitis B Surface Antigen Using Gold Nanoparticles Aggregation and Catalase-Functionalized Polystyrene Nanospheres
title_sort Naked-Eye Detection of Hepatitis B Surface Antigen Using Gold Nanoparticles Aggregation and Catalase-Functionalized Polystyrene Nanospheres
author_id_str_mv b6e81312388169e1da3e505660e08f32
author_id_fullname_str_mv b6e81312388169e1da3e505660e08f32_***_Yufei Liu
author Yufei Liu
author2 Rubing Chen
Yongqin Hu
Meizhu Chen
Jia An
Ying Lyu
Yufei Liu
Dongling Li
format Journal article
container_title ACS Omega
container_volume 6
container_issue 14
container_start_page 9828
publishDate 2021
institution Swansea University
issn 2470-1343
2470-1343
doi_str_mv 10.1021/acsomega.1c00507
publisher American Chemical Society (ACS)
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Uncategorised{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Uncategorised
document_store_str 1
active_str 0
description Developing rapid, efficient, highly sensitive, simple, stable, and low-cost virus marker detection products that are appropriate for basic facilities is of great importance in the early diagnosis and treatment of viruses. Naked-eye detection methods are especially important when medical testing facilities are limited. Polystyrene nanospheres (PSs) with catalytic and specific recognition functions were successfully developed by simultaneously modifying catalase and goat anti-hepatitis B surface antibodies on nanospheres. The modified PSs contributed significantly to the amplification of the signal. Via the specific antigen–antibody reaction, the bifunctional nanospheres could be captured on microplate and then catalyzed the decomposition of hydrogen peroxide to reduce chloroauric acid and synthesize gold nanoparticles (AuNPs). Due to the surface plasmon resonance of AuNPs, the solution color change could be observed with the naked eye and the limit of detection (LOD) was 0.1 ng/mL. Furthermore, the LOD observed with instrumentation was 0.01 ng/mL, which meant that a rapid, efficient, and highly sensitive method for the detection of hepatitis B surface antigens was successfully developed, and neither complex sample pretreatment nor expensive equipment was needed.
published_date 2021-04-13T07:50:33Z
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