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A new high‐resolution melt curve eDNA assay to monitor the simultaneous presence of invasive brown trout ( <i>Salmo trutta</i> ) and endangered galaxiids
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Paul Brickle ,
Sofia Consuegra del Olmo
Environmental DNA, Volume: 3, Issue: 3, Pages: 561 - 572
Swansea University Authors:
Jessica Minett, Carlos Garcia De Leaniz , Sofia Consuegra del Olmo
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DOI (Published version): 10.1002/edn3.151
Abstract
Brown trout are highly invasive in the Southern Hemisphere where they support important sport fisheries and aquaculture activities, which may impact endangered native galaxiid fishes and cause conflicts. To protect native galaxiids it is essential to monitor changes in species distributions, but thi...
| Published in: | Environmental DNA |
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| ISSN: | 2637-4943 2637-4943 |
| Published: |
Wiley
2021
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa56109 |
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| Abstract: |
Brown trout are highly invasive in the Southern Hemisphere where they support important sport fisheries and aquaculture activities, which may impact endangered native galaxiid fishes and cause conflicts. To protect native galaxiids it is essential to monitor changes in species distributions, but this can be difficult when species are rare or difficult to sample. We developed and validated, both in the laboratory and in the field, a new assay using a high-resolution melt curve (HRM)-eDNA approach to simultaneously detect the presence of two threatened native galaxiids (Aplochiton zebra and Aplochiton taeniatus) and the invasive brown trout (Salmo trutta). Using this method, we found brown trout in 30% of the sampled waterbodies and Aplochiton sp. in 15% of them. Galaxiids were solely identified as being present in rivers that lacked brown trout, with both native species coexisting in two of the three rivers where they were detected, despite their different niche preferences. These assays can be used to monitor threatened zebra trout as well as invasive brown trout populations, allowing conservation managers to target areas for intervention. |
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| Keywords: |
Aplochiton taeniatus; Aplochiton zebra; endemic fishes; high‐resolution melt curve; invasive species; qPCR‐HRM |
| College: |
Faculty of Science and Engineering |
| Funders: |
Fortuna Ltd; Swansea University |
| Issue: |
3 |
| Start Page: |
561 |
| End Page: |
572 |