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The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells

Sanni Virjula, Feihu Zhao Orcid Logo, Joni Leivo, Sari Vanhatupa, Joose Kreutzer, Ted J. Vaughan, Anna-Maija Honkala, Marlitt Viehrig, Conleth A. Mullen, Pasi Kallio, Laoise M. McNamara, Susanna Miettinen

Journal of the Mechanical Behavior of Biomedical Materials, Volume: 72, Pages: 38 - 48

Swansea University Author: Feihu Zhao Orcid Logo

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DOI (Published version): 10.1016/j.jmbbm.2017.04.016

Abstract

Although mechanical cues are known to affect stem cell fate and mechanobiology, the significance of such stimuli on the osteogenic differentiation of human adipose stem cells (hASCs) remains unclear. In this study, we investigated the effect of long-term mechanical stimulation on the attachment, ost...

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Published in: Journal of the Mechanical Behavior of Biomedical Materials
ISSN: 1751-6161
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa51687
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spelling 2019-10-11T11:32:19.2831529 v2 51687 2019-09-04 The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells 1c6e79b6edd08c88a8d17a241cd78630 0000-0003-0515-6808 Feihu Zhao Feihu Zhao true false 2019-09-04 MEDE Although mechanical cues are known to affect stem cell fate and mechanobiology, the significance of such stimuli on the osteogenic differentiation of human adipose stem cells (hASCs) remains unclear. In this study, we investigated the effect of long-term mechanical stimulation on the attachment, osteogenic differentiation and mechanical properties of hASCs. Tailor-made, pneumatic cell stretching devices were used to expose hASCs to cyclic equiaxial stretching in osteogenic medium. Cell attachment and focal adhesions were visualised using immunocytochemical vinculin staining on days 3 and 6, and the proliferation and alkaline phosphatase activity, as a sign of early osteogenic differentiation, were analysed on days 0, 6 and 10. Furthermore, the mechanical properties of hASCs, in terms of apparent Young’s modulus and normalised contractility, were obtained using a combination of atomic force microscopy based indentation and computational approaches. Our results indicated that cyclic equiaxial stretching delayed proliferation and promoted osteogenic differentiation of hASCs. Stretching also reduced cell size and intensified focal adhesions and actin cytoskeleton. Moreover, cell stiffening was observed during osteogenic differentiation and especially under mechanical stimulation. These results suggest that cyclic equiaxial stretching modifies cell morphology, focal adhesion formation and mechanical properties of hASCs. This could be exploited to enhance osteogenic differentiation. Journal Article Journal of the Mechanical Behavior of Biomedical Materials 72 38 48 1751-6161 Mesenchymal stem cell, Dynamic cell culture, Mechanical stimulation, Atomic force microscopy indentation, PDMS, mechanobiology 31 8 2017 2017-08-31 10.1016/j.jmbbm.2017.04.016 COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2019-10-11T11:32:19.2831529 2019-09-04T15:40:57.2663884 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Sanni Virjula 1 Feihu Zhao 0000-0003-0515-6808 2 Joni Leivo 3 Sari Vanhatupa 4 Joose Kreutzer 5 Ted J. Vaughan 6 Anna-Maija Honkala 7 Marlitt Viehrig 8 Conleth A. Mullen 9 Pasi Kallio 10 Laoise M. McNamara 11 Susanna Miettinen 12
title The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells
spellingShingle The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells
Feihu Zhao
title_short The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells
title_full The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells
title_fullStr The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells
title_full_unstemmed The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells
title_sort The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells
author_id_str_mv 1c6e79b6edd08c88a8d17a241cd78630
author_id_fullname_str_mv 1c6e79b6edd08c88a8d17a241cd78630_***_Feihu Zhao
author Feihu Zhao
author2 Sanni Virjula
Feihu Zhao
Joni Leivo
Sari Vanhatupa
Joose Kreutzer
Ted J. Vaughan
Anna-Maija Honkala
Marlitt Viehrig
Conleth A. Mullen
Pasi Kallio
Laoise M. McNamara
Susanna Miettinen
format Journal article
container_title Journal of the Mechanical Behavior of Biomedical Materials
container_volume 72
container_start_page 38
publishDate 2017
institution Swansea University
issn 1751-6161
doi_str_mv 10.1016/j.jmbbm.2017.04.016
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
document_store_str 0
active_str 0
description Although mechanical cues are known to affect stem cell fate and mechanobiology, the significance of such stimuli on the osteogenic differentiation of human adipose stem cells (hASCs) remains unclear. In this study, we investigated the effect of long-term mechanical stimulation on the attachment, osteogenic differentiation and mechanical properties of hASCs. Tailor-made, pneumatic cell stretching devices were used to expose hASCs to cyclic equiaxial stretching in osteogenic medium. Cell attachment and focal adhesions were visualised using immunocytochemical vinculin staining on days 3 and 6, and the proliferation and alkaline phosphatase activity, as a sign of early osteogenic differentiation, were analysed on days 0, 6 and 10. Furthermore, the mechanical properties of hASCs, in terms of apparent Young’s modulus and normalised contractility, were obtained using a combination of atomic force microscopy based indentation and computational approaches. Our results indicated that cyclic equiaxial stretching delayed proliferation and promoted osteogenic differentiation of hASCs. Stretching also reduced cell size and intensified focal adhesions and actin cytoskeleton. Moreover, cell stiffening was observed during osteogenic differentiation and especially under mechanical stimulation. These results suggest that cyclic equiaxial stretching modifies cell morphology, focal adhesion formation and mechanical properties of hASCs. This could be exploited to enhance osteogenic differentiation.
published_date 2017-08-31T04:03:41Z
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score 11.013731

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