Journal article 621 views 122 downloads
Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes
Joose Kreutzer,
Marlitt Viehrig,
Risto-Pekka Pölönen,
Feihu Zhao 
,
Marisa Ojala,
Katriina Aalto-Setälä,
Pasi Kallio
,
Marisa Ojala,
Katriina Aalto-Setälä,
Pasi Kallio
Biomechanics and Modeling in Mechanobiology
Swansea University Author:
Feihu Zhao
-
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DOI (Published version): 10.1007/s10237-019-01211-8
Abstract
In this paper, we present a transparent mechanical stimulation device capable of uniaxial stimulation, which is compatible with standard bioanalytical methods used in cellular mechanobiology. We validate the functionality of the uniaxial stimulation system using human-induced pluripotent stem cells-...
| Published in: | Biomechanics and Modeling in Mechanobiology |
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| ISSN: | 1617-7959 1617-7940 |
| Published: |
2019
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa51678 |
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| Abstract: |
In this paper, we present a transparent mechanical stimulation device capable of uniaxial stimulation, which is compatible with standard bioanalytical methods used in cellular mechanobiology. We validate the functionality of the uniaxial stimulation system using human-induced pluripotent stem cells-derived cardiomyocytes (hiPSC-CMs). The pneumatically controlled device is fabricated from polydimethylsiloxane (PDMS) and provides uniaxial strain and superior optical performance compatible with standard inverted microscopy techniques used for bioanalytics (e.g., fluorescence microscopy and calcium imaging). Therefore, it allows for a continuous investigation of the cell state during stretching experiments. The paper introduces design and fabrication of the device, characterizes the mechanical performance of the device and demonstrates the compatibility with standard bioanalytical analysis tools. Imaging modalities, such as high-resolution live cell phase contrast imaging and video recordings, fluorescent imaging and calcium imaging are possible to perform in the device. Utilizing the different imaging modalities and proposed stretching device, we demonstrate the capability of the device for extensive further studies of hiPSC-CMs. We also demonstrate that sarcomere structures of hiPSC-CMs organize and orient perpendicular to uniaxial strain axis and thus express more maturated nature of cardiomyocytes. |
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| Keywords: |
Mechanical stimulation, Cardiomyocytes, hiPSC, PDMS |
| College: |
Faculty of Science and Engineering |