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Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes

Joose Kreutzer, Marlitt Viehrig, Risto-Pekka Pölönen, Feihu Zhao Orcid Logo, Marisa Ojala, Katriina Aalto-Setälä, Pasi Kallio

Biomechanics and Modeling in Mechanobiology

Swansea University Author: Feihu Zhao Orcid Logo

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DOI (Published version): 10.1007/s10237-019-01211-8

Abstract

In this paper, we present a transparent mechanical stimulation device capable of uniaxial stimulation, which is compatible with standard bioanalytical methods used in cellular mechanobiology. We validate the functionality of the uniaxial stimulation system using human-induced pluripotent stem cells-...

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Published in: Biomechanics and Modeling in Mechanobiology
ISSN: 1617-7959 1617-7940
Published: 2019
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URI: https://cronfa.swan.ac.uk/Record/cronfa51678
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first_indexed 2019-09-04T20:46:36Z
last_indexed 2019-10-11T14:23:30Z
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spelling 2019-10-11T10:13:25.0954672 v2 51678 2019-09-04 Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes 1c6e79b6edd08c88a8d17a241cd78630 0000-0003-0515-6808 Feihu Zhao Feihu Zhao true false 2019-09-04 MEDE In this paper, we present a transparent mechanical stimulation device capable of uniaxial stimulation, which is compatible with standard bioanalytical methods used in cellular mechanobiology. We validate the functionality of the uniaxial stimulation system using human-induced pluripotent stem cells-derived cardiomyocytes (hiPSC-CMs). The pneumatically controlled device is fabricated from polydimethylsiloxane (PDMS) and provides uniaxial strain and superior optical performance compatible with standard inverted microscopy techniques used for bioanalytics (e.g., fluorescence microscopy and calcium imaging). Therefore, it allows for a continuous investigation of the cell state during stretching experiments. The paper introduces design and fabrication of the device, characterizes the mechanical performance of the device and demonstrates the compatibility with standard bioanalytical analysis tools. Imaging modalities, such as high-resolution live cell phase contrast imaging and video recordings, fluorescent imaging and calcium imaging are possible to perform in the device. Utilizing the different imaging modalities and proposed stretching device, we demonstrate the capability of the device for extensive further studies of hiPSC-CMs. We also demonstrate that sarcomere structures of hiPSC-CMs organize and orient perpendicular to uniaxial strain axis and thus express more maturated nature of cardiomyocytes. Journal Article Biomechanics and Modeling in Mechanobiology 1617-7959 1617-7940 Mechanical stimulation, Cardiomyocytes, hiPSC, PDMS 31 12 2019 2019-12-31 10.1007/s10237-019-01211-8 COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2019-10-11T10:13:25.0954672 2019-09-04T15:40:39.7935983 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Joose Kreutzer 1 Marlitt Viehrig 2 Risto-Pekka Pölönen 3 Feihu Zhao 0000-0003-0515-6808 4 Marisa Ojala 5 Katriina Aalto-Setälä 6 Pasi Kallio 7 0051678-11102019101316.pdf Kreutzer2019.pdf 2019-10-11T10:13:16.0070000 Output 5161635 application/pdf Version of Record true 2019-10-11T00:00:00.0000000 false eng
title Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes
spellingShingle Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes
Feihu Zhao
title_short Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes
title_full Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes
title_fullStr Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes
title_full_unstemmed Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes
title_sort Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes
author_id_str_mv 1c6e79b6edd08c88a8d17a241cd78630
author_id_fullname_str_mv 1c6e79b6edd08c88a8d17a241cd78630_***_Feihu Zhao
author Feihu Zhao
author2 Joose Kreutzer
Marlitt Viehrig
Risto-Pekka Pölönen
Feihu Zhao
Marisa Ojala
Katriina Aalto-Setälä
Pasi Kallio
format Journal article
container_title Biomechanics and Modeling in Mechanobiology
publishDate 2019
institution Swansea University
issn 1617-7959
1617-7940
doi_str_mv 10.1007/s10237-019-01211-8
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
document_store_str 1
active_str 0
description In this paper, we present a transparent mechanical stimulation device capable of uniaxial stimulation, which is compatible with standard bioanalytical methods used in cellular mechanobiology. We validate the functionality of the uniaxial stimulation system using human-induced pluripotent stem cells-derived cardiomyocytes (hiPSC-CMs). The pneumatically controlled device is fabricated from polydimethylsiloxane (PDMS) and provides uniaxial strain and superior optical performance compatible with standard inverted microscopy techniques used for bioanalytics (e.g., fluorescence microscopy and calcium imaging). Therefore, it allows for a continuous investigation of the cell state during stretching experiments. The paper introduces design and fabrication of the device, characterizes the mechanical performance of the device and demonstrates the compatibility with standard bioanalytical analysis tools. Imaging modalities, such as high-resolution live cell phase contrast imaging and video recordings, fluorescent imaging and calcium imaging are possible to perform in the device. Utilizing the different imaging modalities and proposed stretching device, we demonstrate the capability of the device for extensive further studies of hiPSC-CMs. We also demonstrate that sarcomere structures of hiPSC-CMs organize and orient perpendicular to uniaxial strain axis and thus express more maturated nature of cardiomyocytes.
published_date 2019-12-31T04:03:40Z
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score 11.013148

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