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Surface interaction characterisation of microbial fuel cell organism Shewanella oneidensis. / Maia Kierann Shah

Swansea University Author: Maia Kierann Shah

Abstract

In order to develop MFCs to their full potential, the mechanisms by which organisms such as S. oneidensis transfer electrons extracellularly need to be researched and understood, and key to this are the physical and chemical interactions between the cell surface and the surrounding environment, incl...

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Published: 2011
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
URI: https://cronfa.swan.ac.uk/Record/cronfa42293
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last_indexed 2018-08-03T10:09:46Z
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spelling 2018-08-02T16:24:28.7137853 v2 42293 2018-08-02 Surface interaction characterisation of microbial fuel cell organism Shewanella oneidensis. 0c2159e39419b374c26aa317abf8754b NULL Maia Kierann Shah Maia Kierann Shah true true 2018-08-02 In order to develop MFCs to their full potential, the mechanisms by which organisms such as S. oneidensis transfer electrons extracellularly need to be researched and understood, and key to this are the physical and chemical interactions between the cell surface and the surrounding environment, including other cells, minerals and MFC-relevant substrates. The research presented here characterises the physical interactions of anaerobically grown S. oneidensis MRl under varying chemical conditions, using aerobically grown cells in identical experiments for comparison. An array of experimental methods are used, including techniques for estimating cell concentration for growth profiles, zeta-potential of cells in solution, and Atomic Force Microscopy imaging of cells in different growth phases. A novel method using Surface Plasmon Resonance is used to quantify the kinetics of binding of cells to surfaces approximating MFC electrodes. This method is assessed for suitability and reviewed as a potential answer to other research problems based on cell-device interfaces. Finally, novel force spectroscopy using custom-made mineral probes is used to gather mechanical data about cells of S. oneidensis MR-1 and to quantify the interaction of cells with iron oxide and graphite. The results show the differences in growth profiles between aerobically and anaerobically grown cells. Different results were also seen for aerobically grown and anaerobically grown cells in preliminary SPR studies using poly-L-lysine, and in the force spectroscopy results including adhesion force and Young's moduli. The effects of pH and salinity on cell surface interaction were investigated using measured isoelectric points from the zeta-potential studies as a guide and found to change the measured values of Young's modulus, and the maximum change in SPR response, for both types of cell. The demonstrable effects of ambient chemistry on cell-cell and cell-surface interaction provide a reference point for bio-device design with the potential for multi-organism devices utilising the multiple electron transfer pathways of S. oneidensis MRl. The use of SPR for real time measurement of whole-cell binding to electrode-approximating surfaces and the resultant interaction kinetics is established as a novel, repeatable and accessible way of investigating cell-surface interaction. E-Thesis Bioengineering.;Microbiology.;Alternative Energy. 31 12 2011 2011-12-31 COLLEGE NANME Engineering COLLEGE CODE Swansea University Doctoral Ph.D 2018-08-02T16:24:28.7137853 2018-08-02T16:24:28.7137853 Faculty of Science and Engineering School of Engineering and Applied Sciences - Uncategorised Maia Kierann Shah NULL 1 0042293-02082018162443.pdf 10798001.pdf 2018-08-02T16:24:43.0970000 Output 18259158 application/pdf E-Thesis true 2018-08-02T16:24:43.0970000 false
title Surface interaction characterisation of microbial fuel cell organism Shewanella oneidensis.
spellingShingle Surface interaction characterisation of microbial fuel cell organism Shewanella oneidensis.
Maia Kierann Shah
title_short Surface interaction characterisation of microbial fuel cell organism Shewanella oneidensis.
title_full Surface interaction characterisation of microbial fuel cell organism Shewanella oneidensis.
title_fullStr Surface interaction characterisation of microbial fuel cell organism Shewanella oneidensis.
title_full_unstemmed Surface interaction characterisation of microbial fuel cell organism Shewanella oneidensis.
title_sort Surface interaction characterisation of microbial fuel cell organism Shewanella oneidensis.
author_id_str_mv 0c2159e39419b374c26aa317abf8754b
author_id_fullname_str_mv 0c2159e39419b374c26aa317abf8754b_***_Maia Kierann Shah
author Maia Kierann Shah
author2 Maia Kierann Shah
format E-Thesis
publishDate 2011
institution Swansea University
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Uncategorised{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Uncategorised
document_store_str 1
active_str 0
description In order to develop MFCs to their full potential, the mechanisms by which organisms such as S. oneidensis transfer electrons extracellularly need to be researched and understood, and key to this are the physical and chemical interactions between the cell surface and the surrounding environment, including other cells, minerals and MFC-relevant substrates. The research presented here characterises the physical interactions of anaerobically grown S. oneidensis MRl under varying chemical conditions, using aerobically grown cells in identical experiments for comparison. An array of experimental methods are used, including techniques for estimating cell concentration for growth profiles, zeta-potential of cells in solution, and Atomic Force Microscopy imaging of cells in different growth phases. A novel method using Surface Plasmon Resonance is used to quantify the kinetics of binding of cells to surfaces approximating MFC electrodes. This method is assessed for suitability and reviewed as a potential answer to other research problems based on cell-device interfaces. Finally, novel force spectroscopy using custom-made mineral probes is used to gather mechanical data about cells of S. oneidensis MR-1 and to quantify the interaction of cells with iron oxide and graphite. The results show the differences in growth profiles between aerobically and anaerobically grown cells. Different results were also seen for aerobically grown and anaerobically grown cells in preliminary SPR studies using poly-L-lysine, and in the force spectroscopy results including adhesion force and Young's moduli. The effects of pH and salinity on cell surface interaction were investigated using measured isoelectric points from the zeta-potential studies as a guide and found to change the measured values of Young's modulus, and the maximum change in SPR response, for both types of cell. The demonstrable effects of ambient chemistry on cell-cell and cell-surface interaction provide a reference point for bio-device design with the potential for multi-organism devices utilising the multiple electron transfer pathways of S. oneidensis MRl. The use of SPR for real time measurement of whole-cell binding to electrode-approximating surfaces and the resultant interaction kinetics is established as a novel, repeatable and accessible way of investigating cell-surface interaction.
published_date 2011-12-31T03:52:41Z
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score 11.014067

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