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Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model

F. Ahmad, S. Soe, N. White, R. Johnston, I. Khan, J. Liao, M. Jones, R. Prabhu, I. Maconochie, Peter Theobald, Richard Johnston Orcid Logo, Ilyas Khan Orcid Logo

Annals of Biomedical Engineering

Swansea University Authors: Richard Johnston Orcid Logo, Ilyas Khan Orcid Logo

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DOI (Published version): 10.1007/s10439-018-2089-4

Abstract

The neonate transitions from placenta-derived oxygen, to supply from the pulmonary system, moments after birth. This requires a series of structural developments to divert more blood through the right heart and onto the lungs, with the tissue quickly remodelling to the changing ventricular workload....

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Published in: Annals of Biomedical Engineering
ISSN: 0090-6964 1573-9686
Published: 2018
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URI: https://cronfa.swan.ac.uk/Record/cronfa41043
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This requires a series of structural developments to divert more blood through the right heart and onto the lungs, with the tissue quickly remodelling to the changing ventricular workload. In some cases, however, the heart structure does not fully develop causing poor circulation and inefficient oxygenation, which is associated with an increase in mortality and morbidity. This study focuses on developing an enhanced knowledge of the 1-day old heart, quantifying the region-specific microstructural parameters of the tissue. This will enable more accurate mathematical and computa- tional simulations of the young heart. Hearts were dissected from 12, 1-day-old deceased Yorkshire piglets (mass: 2.1&#x2013; 2.4 kg, length: 0.38&#x2013;0.51 m), acquired from a breeding farm. Evans blue dye was used to label the heart equator and to demarcate the left and right ventricle free walls. Two hearts were used for three-dimensional diffusion-tensor magnetic resonance imaging, to quantify the fractional anisotropy (FA). The remaining hearts were used for two-photon excited fluorescence and second-harmonic generation microscopy, to quantify the cardiomyocyte and collagen fibril structures within the anterior and posterior aspects of the right and left ventricles. FA varied significantly across both ventricles, with the greatest in the equatorial region, followed by the base and apex. The FA in each right ventricular region was statistically greater than that in the left. Cardiomyocyte and collagen fibre rotation was greatest in the anterior wall of both ventricles, with less dispersion when compared to the posterior walls. In defining these key parameters, this study provides a valuable insight into the 1-day-old heart that will provide a valuable platform for further investigation the normal and abnormal heart using mathematical and com- putational models.</abstract><type>Journal Article</type><journal>Annals of Biomedical Engineering</journal><publisher/><issnPrint>0090-6964</issnPrint><issnElectronic>1573-9686</issnElectronic><keywords/><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2018</publishedYear><publishedDate>2018-12-31</publishedDate><doi>10.1007/s10439-018-2089-4</doi><url/><notes/><college>COLLEGE NANME</college><department>Materials Science and Engineering</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>MTLS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2018-09-14T15:35:22.9192538</lastEdited><Created>2018-07-17T15:19:17.1391101</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>F.</firstname><surname>Ahmad</surname><order>1</order></author><author><firstname>S.</firstname><surname>Soe</surname><order>2</order></author><author><firstname>N.</firstname><surname>White</surname><order>3</order></author><author><firstname>R.</firstname><surname>Johnston</surname><order>4</order></author><author><firstname>I.</firstname><surname>Khan</surname><order>5</order></author><author><firstname>J.</firstname><surname>Liao</surname><order>6</order></author><author><firstname>M.</firstname><surname>Jones</surname><order>7</order></author><author><firstname>R.</firstname><surname>Prabhu</surname><order>8</order></author><author><firstname>I.</firstname><surname>Maconochie</surname><order>9</order></author><author><firstname>Peter</firstname><surname>Theobald</surname><order>10</order></author><author><firstname>Richard</firstname><surname>Johnston</surname><orcid>0000-0003-1977-6418</orcid><order>11</order></author><author><firstname>Ilyas</firstname><surname>Khan</surname><orcid>0000-0002-3886-1987</orcid><order>12</order></author></authors><documents><document><filename>0041043-15082018094521.pdf</filename><originalFilename>41043.pdf</originalFilename><uploaded>2018-08-15T09:45:21.5730000</uploaded><type>Output</type><contentLength>5497720</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><embargoDate>2018-07-16T00:00:00.0000000</embargoDate><documentNotes>This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling 2018-09-14T15:35:22.9192538 v2 41043 2018-07-17 Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model 23282e7acce87dd926b8a62ae410a393 0000-0003-1977-6418 Richard Johnston Richard Johnston true false 2536d955ff70e7b77063a8efe9103161 0000-0002-3886-1987 Ilyas Khan Ilyas Khan true false 2018-07-17 MTLS The neonate transitions from placenta-derived oxygen, to supply from the pulmonary system, moments after birth. This requires a series of structural developments to divert more blood through the right heart and onto the lungs, with the tissue quickly remodelling to the changing ventricular workload. In some cases, however, the heart structure does not fully develop causing poor circulation and inefficient oxygenation, which is associated with an increase in mortality and morbidity. This study focuses on developing an enhanced knowledge of the 1-day old heart, quantifying the region-specific microstructural parameters of the tissue. This will enable more accurate mathematical and computa- tional simulations of the young heart. Hearts were dissected from 12, 1-day-old deceased Yorkshire piglets (mass: 2.1– 2.4 kg, length: 0.38–0.51 m), acquired from a breeding farm. Evans blue dye was used to label the heart equator and to demarcate the left and right ventricle free walls. Two hearts were used for three-dimensional diffusion-tensor magnetic resonance imaging, to quantify the fractional anisotropy (FA). The remaining hearts were used for two-photon excited fluorescence and second-harmonic generation microscopy, to quantify the cardiomyocyte and collagen fibril structures within the anterior and posterior aspects of the right and left ventricles. FA varied significantly across both ventricles, with the greatest in the equatorial region, followed by the base and apex. The FA in each right ventricular region was statistically greater than that in the left. Cardiomyocyte and collagen fibre rotation was greatest in the anterior wall of both ventricles, with less dispersion when compared to the posterior walls. In defining these key parameters, this study provides a valuable insight into the 1-day-old heart that will provide a valuable platform for further investigation the normal and abnormal heart using mathematical and com- putational models. Journal Article Annals of Biomedical Engineering 0090-6964 1573-9686 31 12 2018 2018-12-31 10.1007/s10439-018-2089-4 COLLEGE NANME Materials Science and Engineering COLLEGE CODE MTLS Swansea University 2018-09-14T15:35:22.9192538 2018-07-17T15:19:17.1391101 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine F. Ahmad 1 S. Soe 2 N. White 3 R. Johnston 4 I. Khan 5 J. Liao 6 M. Jones 7 R. Prabhu 8 I. Maconochie 9 Peter Theobald 10 Richard Johnston 0000-0003-1977-6418 11 Ilyas Khan 0000-0002-3886-1987 12 0041043-15082018094521.pdf 41043.pdf 2018-08-15T09:45:21.5730000 Output 5497720 application/pdf Version of Record true 2018-07-16T00:00:00.0000000 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License. true eng
title Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model
spellingShingle Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model
Richard Johnston
Ilyas Khan
title_short Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model
title_full Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model
title_fullStr Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model
title_full_unstemmed Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model
title_sort Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model
author_id_str_mv 23282e7acce87dd926b8a62ae410a393
2536d955ff70e7b77063a8efe9103161
author_id_fullname_str_mv 23282e7acce87dd926b8a62ae410a393_***_Richard Johnston
2536d955ff70e7b77063a8efe9103161_***_Ilyas Khan
author Richard Johnston
Ilyas Khan
author2 F. Ahmad
S. Soe
N. White
R. Johnston
I. Khan
J. Liao
M. Jones
R. Prabhu
I. Maconochie
Peter Theobald
Richard Johnston
Ilyas Khan
format Journal article
container_title Annals of Biomedical Engineering
publishDate 2018
institution Swansea University
issn 0090-6964
1573-9686
doi_str_mv 10.1007/s10439-018-2089-4
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description The neonate transitions from placenta-derived oxygen, to supply from the pulmonary system, moments after birth. This requires a series of structural developments to divert more blood through the right heart and onto the lungs, with the tissue quickly remodelling to the changing ventricular workload. In some cases, however, the heart structure does not fully develop causing poor circulation and inefficient oxygenation, which is associated with an increase in mortality and morbidity. This study focuses on developing an enhanced knowledge of the 1-day old heart, quantifying the region-specific microstructural parameters of the tissue. This will enable more accurate mathematical and computa- tional simulations of the young heart. Hearts were dissected from 12, 1-day-old deceased Yorkshire piglets (mass: 2.1– 2.4 kg, length: 0.38–0.51 m), acquired from a breeding farm. Evans blue dye was used to label the heart equator and to demarcate the left and right ventricle free walls. Two hearts were used for three-dimensional diffusion-tensor magnetic resonance imaging, to quantify the fractional anisotropy (FA). The remaining hearts were used for two-photon excited fluorescence and second-harmonic generation microscopy, to quantify the cardiomyocyte and collagen fibril structures within the anterior and posterior aspects of the right and left ventricles. FA varied significantly across both ventricles, with the greatest in the equatorial region, followed by the base and apex. The FA in each right ventricular region was statistically greater than that in the left. Cardiomyocyte and collagen fibre rotation was greatest in the anterior wall of both ventricles, with less dispersion when compared to the posterior walls. In defining these key parameters, this study provides a valuable insight into the 1-day-old heart that will provide a valuable platform for further investigation the normal and abnormal heart using mathematical and com- putational models.
published_date 2018-12-31T03:52:18Z
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score 11.037603

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