Journal article 1497 views 193 downloads
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
European Cells and Materials, Volume: 35, Pages: 132 - 150
Swansea University Author:
Ilyas Khan
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DOI (Published version): 10.22203/eCM.v035a10
Abstract
Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine str...
| Published in: | European Cells and Materials |
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| Published: |
2018
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa41044 |
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| Abstract: |
Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs). However, the quality of neo-tissues resulting from these cell types is inadequate due to ine cient chondrogenic di erentiation and endochondral ossi cation, respectively. Recently, a subpopulation of stem/progenitor cells has been identi ed within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored. This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic di erentiation. Neocartilage formation was assessed through gene expression pro ling, compression testing, biochemical composition and histology. Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties. AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes. These results underscored the AuCPCs’ tissue-speci c di erentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs. |
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| College: |
Faculty of Medicine, Health and Life Sciences |
| Start Page: |
132 |
| End Page: |
150 |