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In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models

Sylvia Lehmann, Benjamin Gilbert, Thierry Maffeis Orcid Logo, Alexei Grichine, Isabelle Pignot-Paintrand, Simon Clavaguera, Walid Rachidi, Michel Seve, Laurent Charlet

Nanomaterials, Volume: 8, Issue: 4, Start page: 232

Swansea University Author: Thierry Maffeis Orcid Logo

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DOI (Published version): 10.3390/nano8040232

Abstract

Silver nanowires (AgNW) are attractive materials that are anticipated to be incorporated into numerous consumer products such as textiles, touchscreen display, and medical devices that could be in direct contact with skin. There are very few studies on the cellular toxicity of AgNW and no studies th...

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Published in: Nanomaterials
ISSN: 2079-4991
Published: 2018
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URI: https://cronfa.swan.ac.uk/Record/cronfa39989
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spelling 2018-08-06T10:04:35.0384876 v2 39989 2018-05-08 In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models 992eb4cb18b61c0cd3da6e0215ac787c 0000-0003-2357-0092 Thierry Maffeis Thierry Maffeis true false 2018-05-08 EEEG Silver nanowires (AgNW) are attractive materials that are anticipated to be incorporated into numerous consumer products such as textiles, touchscreen display, and medical devices that could be in direct contact with skin. There are very few studies on the cellular toxicity of AgNW and no studies that have specifically evaluated the potential toxicity from dermal exposure. To address this question, we investigated the dermal toxicity after acute exposure of polymer-coated AgNW with two sizes using two models, human primary keratinocytes and human reconstructed epidermis. In keratinocytes, AgNW are rapidly and massively internalized inside cells leading to dose-dependent cytotoxicity that was not due to Ag+ release. Analysing our data with different dose metrics, we propose that the number of NW is the most appropriate dose-metric for studies of AgNW toxicity. In reconstructed epidermis, the results of a standard in vitro skin irritation assay classified AgNW as non-irritant to skin and we found no evidence of penetration into the deeper layer of the epidermis. The findings show that healthy and intact epidermis provides an effective barrier for AgNW, although the study does not address potential transport through follicles or injured skin. The combined cell and tissue model approach used here is likely to provide an important methodology for assessing the risks for skin exposure to AgNW from consumer products. Journal Article Nanomaterials 8 4 232 2079-4991 silver nanowires; cytotoxicity; skin irritation in vitro; primary keratinocytes; 3D reconstructed epidermis model 11 4 2018 2018-04-11 10.3390/nano8040232 This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0). COLLEGE NANME Electronic and Electrical Engineering COLLEGE CODE EEEG Swansea University 2018-08-06T10:04:35.0384876 2018-05-08T09:16:32.3388022 Faculty of Science and Engineering School of Aerospace, Civil, Electrical, General and Mechanical Engineering - Electronic and Electrical Engineering Sylvia Lehmann 1 Benjamin Gilbert 2 Thierry Maffeis 0000-0003-2357-0092 3 Alexei Grichine 4 Isabelle Pignot-Paintrand 5 Simon Clavaguera 6 Walid Rachidi 7 Michel Seve 8 Laurent Charlet 9 0039989-08052018091848.pdf lehmann2018.pdf 2018-05-08T09:18:48.2330000 Output 17112606 application/pdf Version of Record true 2018-05-08T00:00:00.0000000 true eng
title In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models
spellingShingle In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models
Thierry Maffeis
title_short In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models
title_full In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models
title_fullStr In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models
title_full_unstemmed In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models
title_sort In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models
author_id_str_mv 992eb4cb18b61c0cd3da6e0215ac787c
author_id_fullname_str_mv 992eb4cb18b61c0cd3da6e0215ac787c_***_Thierry Maffeis
author Thierry Maffeis
author2 Sylvia Lehmann
Benjamin Gilbert
Thierry Maffeis
Alexei Grichine
Isabelle Pignot-Paintrand
Simon Clavaguera
Walid Rachidi
Michel Seve
Laurent Charlet
format Journal article
container_title Nanomaterials
container_volume 8
container_issue 4
container_start_page 232
publishDate 2018
institution Swansea University
issn 2079-4991
doi_str_mv 10.3390/nano8040232
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Aerospace, Civil, Electrical, General and Mechanical Engineering - Electronic and Electrical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Aerospace, Civil, Electrical, General and Mechanical Engineering - Electronic and Electrical Engineering
document_store_str 1
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description Silver nanowires (AgNW) are attractive materials that are anticipated to be incorporated into numerous consumer products such as textiles, touchscreen display, and medical devices that could be in direct contact with skin. There are very few studies on the cellular toxicity of AgNW and no studies that have specifically evaluated the potential toxicity from dermal exposure. To address this question, we investigated the dermal toxicity after acute exposure of polymer-coated AgNW with two sizes using two models, human primary keratinocytes and human reconstructed epidermis. In keratinocytes, AgNW are rapidly and massively internalized inside cells leading to dose-dependent cytotoxicity that was not due to Ag+ release. Analysing our data with different dose metrics, we propose that the number of NW is the most appropriate dose-metric for studies of AgNW toxicity. In reconstructed epidermis, the results of a standard in vitro skin irritation assay classified AgNW as non-irritant to skin and we found no evidence of penetration into the deeper layer of the epidermis. The findings show that healthy and intact epidermis provides an effective barrier for AgNW, although the study does not address potential transport through follicles or injured skin. The combined cell and tissue model approach used here is likely to provide an important methodology for assessing the risks for skin exposure to AgNW from consumer products.
published_date 2018-04-11T03:50:51Z
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