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Limitations in the use of PSMγ, agr , RNAIII, and biofilm formation as biomarkers to define invasive Staphylococcus epidermidis from chronic biomedical device-associated infections

Llinos Harris Orcid Logo, Ed Dudley, Holger Rohde, Lars Frommelt, Nicolaus Siemssen, Thomas Wilkinson Orcid Logo, Dietrich Mack

International Journal of Medical Microbiology, Volume: 307, Issue: 7, Pages: 382 - 387

Swansea University Authors: Llinos Harris Orcid Logo, Ed Dudley, Thomas Wilkinson Orcid Logo, Dietrich Mack

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DOI (Published version): 10.1016/j.ijmm.2017.08.003

Abstract

Staphylococcus epidermidis is a common cause of biomedical device-associated infections. Agr is the major quorum sensing system in staphylococci and regulates virulence factors. Four agr-specificity groups exist in S. epidermidis, and chronic S. epidermidis infections are hypothesised to select for...

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Published in: International Journal of Medical Microbiology
ISSN: 1438-4221
Published: Elsevier BV 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa35039
first_indexed 2017-08-30T13:01:01Z
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Agr is the major quorum sensing system in staphylococci and regulates virulence factors. Four agr-specificity groups exist in S. epidermidis, and chronic S. epidermidis infections are hypothesised to select for agr-negative phenotypes. Therefore, we investigated S. epidermidis strains from prosthetic joint- and catheter-associated infections to establish i) whether an infection selects for an agr-negative phenotype; ii) the importance of PSM&#x3B3; and iii) if the agr-specificity group is infection dependent. S. epidermidis nasal isolates from healthy volunteers were used as controls. The distribution of agr-specificity groups was significantly different between infection and control episodes, but did not distinguish between the infection types. PSM&#x3B3; secretion was used to determine agr-activity and HPLC analysis showed that 44% of prosthetic and 32% of catheter-associated episodes produced no PSM&#x3B3; in comparison to 8% of the control strains. However, PSM&#x3B3; expression did not always correlate with RNAIII up-regulation, indicating that PSM&#x3B3; synthesis is likely influenced by additional post-transcriptional control. The data suggests chronic S. epidermidis infections favour agr-specificity group 1 but the results suggest that they do not select for an agr-negative phenotype. 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spelling 2022-01-18T10:39:08.2801850 v2 35039 2017-08-30 Limitations in the use of PSMγ, agr , RNAIII, and biofilm formation as biomarkers to define invasive Staphylococcus epidermidis from chronic biomedical device-associated infections dc70f9d4badbbdb5d467fd321986d173 0000-0002-0295-3038 Llinos Harris Llinos Harris true false c7d05f992a817cd3b9a5f946bd909b71 Ed Dudley Ed Dudley true false 86cca6bf31bfe8572de27c1b441420d8 0000-0003-0397-6079 Thomas Wilkinson Thomas Wilkinson true false c6afa8ec5adb139f55d50550e0d9f03f Dietrich Mack Dietrich Mack true false 2017-08-30 MEDS Staphylococcus epidermidis is a common cause of biomedical device-associated infections. Agr is the major quorum sensing system in staphylococci and regulates virulence factors. Four agr-specificity groups exist in S. epidermidis, and chronic S. epidermidis infections are hypothesised to select for agr-negative phenotypes. Therefore, we investigated S. epidermidis strains from prosthetic joint- and catheter-associated infections to establish i) whether an infection selects for an agr-negative phenotype; ii) the importance of PSMγ and iii) if the agr-specificity group is infection dependent. S. epidermidis nasal isolates from healthy volunteers were used as controls. The distribution of agr-specificity groups was significantly different between infection and control episodes, but did not distinguish between the infection types. PSMγ secretion was used to determine agr-activity and HPLC analysis showed that 44% of prosthetic and 32% of catheter-associated episodes produced no PSMγ in comparison to 8% of the control strains. However, PSMγ expression did not always correlate with RNAIII up-regulation, indicating that PSMγ synthesis is likely influenced by additional post-transcriptional control. The data suggests chronic S. epidermidis infections favour agr-specificity group 1 but the results suggest that they do not select for an agr-negative phenotype. Further studies are required to explore the mechanisms underlying the selection and survival of these S. epidermidis phenotypes isolated from biomedical device-associated infections. Journal Article International Journal of Medical Microbiology 307 7 382 387 Elsevier BV 1438-4221 Staphylococcus epidermidis; agr; Quorum sensing; Infection; Phenol soluble modulins; Biomedical devices; Biofilm formation 1 10 2017 2017-10-01 10.1016/j.ijmm.2017.08.003 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2022-01-18T10:39:08.2801850 2017-08-30T10:38:19.3572523 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Llinos Harris 0000-0002-0295-3038 1 Ed Dudley 2 Holger Rohde 3 Lars Frommelt 4 Nicolaus Siemssen 5 Thomas Wilkinson 0000-0003-0397-6079 6 Dietrich Mack 7 0035039-27092017143539.pdf 35039.pdf 2017-09-27T14:35:39.3570000 Output 685659 application/pdf Accepted Manuscript true 2018-08-15T00:00:00.0000000 ©2017 All rights reserved. All article content, except where otherwise noted, is licensed under a Creative Commons Attribution Non-Commercial No Derivatives License (CC-BY-NC-ND) true eng https://creativecommons.org/licenses/by-nc-nd/4.0/
title Limitations in the use of PSMγ, agr , RNAIII, and biofilm formation as biomarkers to define invasive Staphylococcus epidermidis from chronic biomedical device-associated infections
spellingShingle Limitations in the use of PSMγ, agr , RNAIII, and biofilm formation as biomarkers to define invasive Staphylococcus epidermidis from chronic biomedical device-associated infections
Llinos Harris
Ed Dudley
Thomas Wilkinson
Dietrich Mack
title_short Limitations in the use of PSMγ, agr , RNAIII, and biofilm formation as biomarkers to define invasive Staphylococcus epidermidis from chronic biomedical device-associated infections
title_full Limitations in the use of PSMγ, agr , RNAIII, and biofilm formation as biomarkers to define invasive Staphylococcus epidermidis from chronic biomedical device-associated infections
title_fullStr Limitations in the use of PSMγ, agr , RNAIII, and biofilm formation as biomarkers to define invasive Staphylococcus epidermidis from chronic biomedical device-associated infections
title_full_unstemmed Limitations in the use of PSMγ, agr , RNAIII, and biofilm formation as biomarkers to define invasive Staphylococcus epidermidis from chronic biomedical device-associated infections
title_sort Limitations in the use of PSMγ, agr , RNAIII, and biofilm formation as biomarkers to define invasive Staphylococcus epidermidis from chronic biomedical device-associated infections
author_id_str_mv dc70f9d4badbbdb5d467fd321986d173
c7d05f992a817cd3b9a5f946bd909b71
86cca6bf31bfe8572de27c1b441420d8
c6afa8ec5adb139f55d50550e0d9f03f
author_id_fullname_str_mv dc70f9d4badbbdb5d467fd321986d173_***_Llinos Harris
c7d05f992a817cd3b9a5f946bd909b71_***_Ed Dudley
86cca6bf31bfe8572de27c1b441420d8_***_Thomas Wilkinson
c6afa8ec5adb139f55d50550e0d9f03f_***_Dietrich Mack
author Llinos Harris
Ed Dudley
Thomas Wilkinson
Dietrich Mack
author2 Llinos Harris
Ed Dudley
Holger Rohde
Lars Frommelt
Nicolaus Siemssen
Thomas Wilkinson
Dietrich Mack
format Journal article
container_title International Journal of Medical Microbiology
container_volume 307
container_issue 7
container_start_page 382
publishDate 2017
institution Swansea University
issn 1438-4221
doi_str_mv 10.1016/j.ijmm.2017.08.003
publisher Elsevier BV
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description Staphylococcus epidermidis is a common cause of biomedical device-associated infections. Agr is the major quorum sensing system in staphylococci and regulates virulence factors. Four agr-specificity groups exist in S. epidermidis, and chronic S. epidermidis infections are hypothesised to select for agr-negative phenotypes. Therefore, we investigated S. epidermidis strains from prosthetic joint- and catheter-associated infections to establish i) whether an infection selects for an agr-negative phenotype; ii) the importance of PSMγ and iii) if the agr-specificity group is infection dependent. S. epidermidis nasal isolates from healthy volunteers were used as controls. The distribution of agr-specificity groups was significantly different between infection and control episodes, but did not distinguish between the infection types. PSMγ secretion was used to determine agr-activity and HPLC analysis showed that 44% of prosthetic and 32% of catheter-associated episodes produced no PSMγ in comparison to 8% of the control strains. However, PSMγ expression did not always correlate with RNAIII up-regulation, indicating that PSMγ synthesis is likely influenced by additional post-transcriptional control. The data suggests chronic S. epidermidis infections favour agr-specificity group 1 but the results suggest that they do not select for an agr-negative phenotype. Further studies are required to explore the mechanisms underlying the selection and survival of these S. epidermidis phenotypes isolated from biomedical device-associated infections.
published_date 2017-10-01T19:11:18Z
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