Journal article 1535 views 378 downloads
Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
April Rees 
,
Louis M. Scott,
Aled Bryant ,
April Rees,
Billy Down,
Ruth Jones ,
Cathy Thornton
,
Louis M. Scott,
Aled Bryant ,
April Rees,
Billy Down,
Ruth Jones ,
Cathy Thornton
Cytokine, Volume: 99, Pages: 194 - 202
Swansea University Authors:
April Rees , Aled Bryant , Ruth Jones , Cathy Thornton
-
PDF | Accepted Manuscript
Download (850.43KB)
DOI (Published version): 10.1016/j.cyto.2017.07.005
Abstract
IL-1 family members regulate innate immune responses, are produced by gestation-associated tissues, and have a role in healthy and adverse pregnancy outcomes. To better understand their role at the materno-fetal interface we used a human tissue explant model to map lipopolysaccharide (LPS)-stimulate...
| Published in: | Cytokine |
|---|---|
| ISSN: | 10434666 |
| Published: |
2017
|
| Online Access: |
Check full text
|
| URI: | https://cronfa.swan.ac.uk/Record/cronfa34588 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract: |
IL-1 family members regulate innate immune responses, are produced by gestation-associated tissues, and have a role in healthy and adverse pregnancy outcomes. To better understand their role at the materno-fetal interface we used a human tissue explant model to map lipopolysaccharide (LPS)-stimulated production of IL-1α, IL-1β, IL-18, IL-33, IL-1Ra, IL-18BPa, ST2 and IL-1RAcP by placenta, choriodecidua and amnion. Caspase-dependent processing of IL-1α, IL-1β, IL-18, and IL-33 and the ability of IL-1α, IL-1β, IL-18, and IL-33 to regulate the production of IL-1RA, IL-18BPa, ST2 and IL-1RAcP was also determined. LPS acted as a potent inducer of IL-1 family member expression especially in the placenta and choriodecidua with the response by the amnion restricted to IL-1β. Caspases-1, 4 and 8 contributed to LPS-stimulated production of IL-1 and IL-18, whereas calpain was required for IL-1 production. Exogenous administration of IL-1α, IL-1β, IL-18, and IL-33 lead to differential expression of IL-1Ra, IL-18BPa, ST2 and IL-1RAcP across all tissues examined. Most notable were the counter-regulatory effect of LPS on IL-1 and IL-1Ra in the amnion and the broad responsiveness of the amnion to IL-1 family cytokines for increased production of immunomodulatory peptides and soluble receptors. The placenta and membranes vary not only in their output of various IL-1 family members but also in their counter-regulatory mechanisms through endogenous inhibitory peptides, processing enzymes and soluble decoy receptors. This interactive network of inflammatory mediators likely contributes to innate defence mechanisms at the materno-fetal interface to limit, in particular, the detrimental effects of microbial invasion. |
|---|---|
| Keywords: |
Cytokines, IL-1 family, Placenta, Preterm labour, Reproductive Immunology. |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Start Page: |
194 |
| End Page: |
202 |