Journal article 1520 views 383 downloads
Expression and function of NOD-like receptors by human term gestation-associated tissues
,
Ryan J. Bevan,
Samantha Spencer-Harty,
Louis M. Scott,
Ruth H. Jones,
Cathy Thornton
Placenta, Volume: 58, Pages: 25 - 32
Swansea University Authors:
Aled Bryant , Cathy Thornton
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DOI (Published version): 10.1016/j.placenta.2017.07.017
Abstract
Introduction: Nucleotide-binding oligomerization domain (NOD)-like receptors or NOD-like receptors (NLRs) have been implicated in several disease pathologies associated with inflammation. Since local and systemic inflammation is a hallmark of both term and preterm labour, a role for NLRs at the mate...
| Published in: | Placenta |
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| ISSN: | 01434004 |
| Published: |
2017
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa34833 |
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| Abstract: |
Introduction: Nucleotide-binding oligomerization domain (NOD)-like receptors or NOD-like receptors (NLRs) have been implicated in several disease pathologies associated with inflammation. Since local and systemic inflammation is a hallmark of both term and preterm labour, a role for NLRs at the materno-fetal interface has been postulated. Methods: Gene expression and immunolocalisation of NLR family members in human placenta, choriodecidua, and amnion were examined. Tissue explants were used to examine the response to activators of NOD1 (Tri-DAP), NOD2 (MDP) and NLRP3 (nigericin). Cell/tissue-free supernatants were examined for the production of interleukin (IL)-1, IL-6, IL-8 and IL-10 using specific ELISAs. Results: Expression of transcripts for NOD1, NOD2, NLRP3, NLRC4, NLRX1, NLRP1 and NAIP and protein expression of NOD1, NOD2 and NLRP3 were a broad feature of all term gestation-associated tissues. Production of cytokines was increased significantly in response to all ligands in placenta and choriodecidua, except for MDP-induced IL-10. Similarly, there was a significant increase in cytokine production in the amnion except for MDP induced IL-1β and IL-10 response to either agonist. IL-1β production was dependent on caspase-1 regardless of agonist used or tissue examined.Discussion: Term human gestation-associated tissues express functional NLRs which likely play a role in both sterile and pathogen-driven inflammatory responses at the materno-fetal interface. |
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| Keywords: |
Inflammation; reproductive immunology; preterm labour; NOD-like receptors |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Start Page: |
25 |
| End Page: |
32 |