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Dynasore - not just a dynamin inhibitor

Giulio Preta, James Cronin Orcid Logo, I Martin Sheldon, Martin Sheldon Orcid Logo

Cell Communication and Signaling, Volume: 13, Issue: 1

Swansea University Authors: James Cronin Orcid Logo, Martin Sheldon Orcid Logo

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DOI (Published version): 10.1186/s12964-015-0102-1

Abstract

Dynamin is a GTPase protein that is essential for membrane fission during clathrin-mediated endocytosis in eukaryotic cells. Dynasore is a GTPase inhibitor that rapidly and reversibly inhibits dynamin activity, which prevents endocytosis. However, comparison between cells treated with dynasore and R...

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Published in: Cell Communication and Signaling
ISSN: 1478-811X
Published: 2015
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URI: https://cronfa.swan.ac.uk/Record/cronfa20642
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first_indexed 2015-04-14T02:06:58Z
last_indexed 2019-10-14T13:27:13Z
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spelling 2019-10-14T10:52:24.4518323 v2 20642 2015-04-13 Dynasore - not just a dynamin inhibitor 9cfd17551c0d1f7438895121e4fbb6e8 0000-0002-0590-9462 James Cronin James Cronin true false ab0f74b794e59cc270c69e63ee1d9748 0000-0001-7902-5558 Martin Sheldon Martin Sheldon true false 2015-04-13 BMS Dynamin is a GTPase protein that is essential for membrane fission during clathrin-mediated endocytosis in eukaryotic cells. Dynasore is a GTPase inhibitor that rapidly and reversibly inhibits dynamin activity, which prevents endocytosis. However, comparison between cells treated with dynasore and RNA interference of genes encoding dynamin, reveals evidence that dynasore reduces labile cholesterol in the plasma membrane, and disrupts lipid raft organization, in a dynamin-independent manner. To explore the role of dynamin it is important to use multiple dynamin inhibitors, alongside the use of dynamin mutants and RNA interference targeting genes encoding dynamin. On the other hand, dynasore provides an interesting tool to explore the regulation of cholesterol in plasma membranes. Journal Article Cell Communication and Signaling 13 1 1478-811X 13 4 2015 2015-04-13 10.1186/s12964-015-0102-1 http://www.biosignaling.com/content/pdf/s12964-015-0102-1.pdf COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2019-10-14T10:52:24.4518323 2015-04-13T19:59:53.0280192 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Giulio Preta 1 James Cronin 0000-0002-0590-9462 2 I Martin Sheldon 3 Martin Sheldon 0000-0001-7902-5558 4 0020642-13042015200106.pdf s12964-015-0102-1.pdf 2015-04-13T20:01:06.4870000 Output 1357605 application/pdf Version of Record true 2015-04-13T00:00:00.0000000 Distributed under the terms of a Creative Commons Attribution (CC-BY-4.0) true
title Dynasore - not just a dynamin inhibitor
spellingShingle Dynasore - not just a dynamin inhibitor
James Cronin
Martin Sheldon
title_short Dynasore - not just a dynamin inhibitor
title_full Dynasore - not just a dynamin inhibitor
title_fullStr Dynasore - not just a dynamin inhibitor
title_full_unstemmed Dynasore - not just a dynamin inhibitor
title_sort Dynasore - not just a dynamin inhibitor
author_id_str_mv 9cfd17551c0d1f7438895121e4fbb6e8
ab0f74b794e59cc270c69e63ee1d9748
author_id_fullname_str_mv 9cfd17551c0d1f7438895121e4fbb6e8_***_James Cronin
ab0f74b794e59cc270c69e63ee1d9748_***_Martin Sheldon
author James Cronin
Martin Sheldon
author2 Giulio Preta
James Cronin
I Martin Sheldon
Martin Sheldon
format Journal article
container_title Cell Communication and Signaling
container_volume 13
container_issue 1
publishDate 2015
institution Swansea University
issn 1478-811X
doi_str_mv 10.1186/s12964-015-0102-1
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url http://www.biosignaling.com/content/pdf/s12964-015-0102-1.pdf
document_store_str 1
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description Dynamin is a GTPase protein that is essential for membrane fission during clathrin-mediated endocytosis in eukaryotic cells. Dynasore is a GTPase inhibitor that rapidly and reversibly inhibits dynamin activity, which prevents endocytosis. However, comparison between cells treated with dynasore and RNA interference of genes encoding dynamin, reveals evidence that dynasore reduces labile cholesterol in the plasma membrane, and disrupts lipid raft organization, in a dynamin-independent manner. To explore the role of dynamin it is important to use multiple dynamin inhibitors, alongside the use of dynamin mutants and RNA interference targeting genes encoding dynamin. On the other hand, dynasore provides an interesting tool to explore the regulation of cholesterol in plasma membranes.
published_date 2015-04-13T03:24:26Z
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score 11.017016

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