24S,25-Epoxycholesterol in mouse and rat brain

Wang, Yuchen; Karu, Kersti; Meljon, Anna; Turton, John; Yau, Joyce L.; Seckl, Jonathan R.; Wang, Yuqin; Griffiths, William

doi:10.1016/j.bbrc.2014.05.012

Journal article 1270 views

24S,25-Epoxycholesterol in mouse and rat brain

Yuchen Wang, Kersti Karu, Anna Meljon, John Turton, Joyce L. Yau, Jonathan R. Seckl, Yuqin Wang

, William Griffiths

Biochemical and Biophysical Research Communications

Swansea University Authors: Yuqin Wang , William Griffiths

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DOI (Published version): 10.1016/j.bbrc.2014.05.012

Abstract

24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high se...

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Published in:	Biochemical and Biophysical Research Communications
Published:	2014
URI:	https://cronfa.swan.ac.uk/Record/cronfa17998
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first_indexed	2014-05-29T10:24:50Z
last_indexed	2018-02-09T04:52:10Z
id	cronfa17998
recordtype	SURis
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spelling	2014-05-22T10:09:20.9285633 v2 17998 2014-05-22 24S,25-Epoxycholesterol in mouse and rat brain c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2014-05-22 BMS 24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of the order of 0.4-1.4μg/g wet weight in both adult mouse and rat. For comparison the level of 24S-hydroxycholesterol in brain of both rodents was of the order of 20μg/g, while that of cholesterol in mouse was 10-20mg/g. By exploiting knockout mice for the enzyme oxysterol 7α-hydroxylase (Cyp7b1) we show that this enzymes is important for the subsequent metabolism of the 24S,25-epoxide Journal Article Biochemical and Biophysical Research Communications 31 12 2014 2014-12-31 10.1016/j.bbrc.2014.05.012 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2014-05-22T10:09:20.9285633 2014-05-22T10:09:20.9285633 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Yuchen Wang 1 Kersti Karu 2 Anna Meljon 3 John Turton 4 Joyce L. Yau 5 Jonathan R. Seckl 6 Yuqin Wang 0000-0002-3063-3066 7 William Griffiths 0000-0002-4129-6616 8
title	24S,25-Epoxycholesterol in mouse and rat brain
spellingShingle	24S,25-Epoxycholesterol in mouse and rat brain Yuqin Wang William Griffiths
title_short	24S,25-Epoxycholesterol in mouse and rat brain
title_full	24S,25-Epoxycholesterol in mouse and rat brain
title_fullStr	24S,25-Epoxycholesterol in mouse and rat brain
title_full_unstemmed	24S,25-Epoxycholesterol in mouse and rat brain
title_sort	24S,25-Epoxycholesterol in mouse and rat brain
author_id_str_mv	c92729b58622f9fdf6a0e7d8f4ce5081 3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv	c92729b58622f9fdf6a0e7d8f4ce5081_*_Yuqin Wang 3316b1d1b524be1831790933eed1c26e_*_William Griffiths
author	Yuqin Wang William Griffiths
author2	Yuchen Wang Kersti Karu Anna Meljon John Turton Joyce L. Yau Jonathan R. Seckl Yuqin Wang William Griffiths
format	Journal article
container_title	Biochemical and Biophysical Research Communications
publishDate	2014
institution	Swansea University
doi_str_mv	10.1016/j.bbrc.2014.05.012
college_str	Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id	facultyofmedicinehealthandlifesciences
hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str	0
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description	24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of the order of 0.4-1.4μg/g wet weight in both adult mouse and rat. For comparison the level of 24S-hydroxycholesterol in brain of both rodents was of the order of 20μg/g, while that of cholesterol in mouse was 10-20mg/g. By exploiting knockout mice for the enzyme oxysterol 7α-hydroxylase (Cyp7b1) we show that this enzymes is important for the subsequent metabolism of the 24S,25-epoxide
published_date	2014-12-31T03:20:59Z
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score	11.037581

24S,25-Epoxycholesterol in mouse and rat brain

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