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A new derivative for oxosteroid analysis by mass spectrometry

, William Griffiths Orcid Logo, Yuqin Wang Orcid Logo

Biochemical and Biophysical Research Communications

Swansea University Authors: William Griffiths Orcid Logo, Yuqin Wang Orcid Logo

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DOI (Published version): 10.1016/j.bbrc.2014.01.190

Abstract

Here we report a new method for oxosteroid identification utilizing "tandem mass tag hydrazine" (TMTH) carbonyl-reactive derivatisation reagent. TMTH is a reagent with a chargeable tertiary amino group attached through a linker to a carbonyl-reactive hydrazine group. Thirty oxosteroids wer...

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Published in: Biochemical and Biophysical Research Communications
Published: 2014
URI: https://cronfa.swan.ac.uk/Record/cronfa17815
first_indexed 2014-04-15T01:30:03Z
last_indexed 2018-02-09T04:51:49Z
id cronfa17815
recordtype SURis
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spelling 2014-04-14T16:41:01.6263853 v2 17815 2014-04-14 A new derivative for oxosteroid analysis by mass spectrometry 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 2014-04-14 MEDS Here we report a new method for oxosteroid identification utilizing "tandem mass tag hydrazine" (TMTH) carbonyl-reactive derivatisation reagent. TMTH is a reagent with a chargeable tertiary amino group attached through a linker to a carbonyl-reactive hydrazine group. Thirty oxosteroids were analysed after derivatisation with TMTH by electrospray ionization mass spectrometry (ESI-MS) and were found to give high ion-currents compared to underivatised molecules. ESI-tandem mass spectrometry (MS/MS) analysis of the derivatives yielded characteristic fragmentation patterns with specific mass reporter ions derived from the TMT group. A shotgun ESI-MS method incorporating TMTH derivatisation was applied to a urine sample Journal Article Biochemical and Biophysical Research Communications 31 12 2014 2014-12-31 10.1016/j.bbrc.2014.01.190 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2014-04-14T16:41:01.6263853 2014-04-14T16:41:01.6263853 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine 1 William Griffiths 0000-0002-4129-6616 2 Yuqin Wang 0000-0002-3063-3066 3
title A new derivative for oxosteroid analysis by mass spectrometry
spellingShingle A new derivative for oxosteroid analysis by mass spectrometry
William Griffiths
Yuqin Wang
title_short A new derivative for oxosteroid analysis by mass spectrometry
title_full A new derivative for oxosteroid analysis by mass spectrometry
title_fullStr A new derivative for oxosteroid analysis by mass spectrometry
title_full_unstemmed A new derivative for oxosteroid analysis by mass spectrometry
title_sort A new derivative for oxosteroid analysis by mass spectrometry
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
c92729b58622f9fdf6a0e7d8f4ce5081
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William Griffiths
c92729b58622f9fdf6a0e7d8f4ce5081_***_Yuqin Wang
author William Griffiths
Yuqin Wang
author2
William Griffiths
Yuqin Wang
format Journal article
container_title Biochemical and Biophysical Research Communications
publishDate 2014
institution Swansea University
doi_str_mv 10.1016/j.bbrc.2014.01.190
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
active_str 0
description Here we report a new method for oxosteroid identification utilizing "tandem mass tag hydrazine" (TMTH) carbonyl-reactive derivatisation reagent. TMTH is a reagent with a chargeable tertiary amino group attached through a linker to a carbonyl-reactive hydrazine group. Thirty oxosteroids were analysed after derivatisation with TMTH by electrospray ionization mass spectrometry (ESI-MS) and were found to give high ion-currents compared to underivatised molecules. ESI-tandem mass spectrometry (MS/MS) analysis of the derivatives yielded characteristic fragmentation patterns with specific mass reporter ions derived from the TMT group. A shotgun ESI-MS method incorporating TMTH derivatisation was applied to a urine sample
published_date 2014-12-31T03:33:23Z
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score 11.089386

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