Journal article 1117 views
A new derivative for oxosteroid analysis by mass spectrometry
,
William Griffiths 
,
Yuqin Wang
,
Yuqin Wang
Biochemical and Biophysical Research Communications
Swansea University Authors:
William Griffiths , Yuqin Wang
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DOI (Published version): 10.1016/j.bbrc.2014.01.190
Abstract
Here we report a new method for oxosteroid identification utilizing "tandem mass tag hydrazine" (TMTH) carbonyl-reactive derivatisation reagent. TMTH is a reagent with a chargeable tertiary amino group attached through a linker to a carbonyl-reactive hydrazine group. Thirty oxosteroids wer...
| Published in: | Biochemical and Biophysical Research Communications |
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| Published: |
2014
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa17815 |
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| Abstract: |
Here we report a new method for oxosteroid identification utilizing "tandem mass tag hydrazine" (TMTH) carbonyl-reactive derivatisation reagent. TMTH is a reagent with a chargeable tertiary amino group attached through a linker to a carbonyl-reactive hydrazine group. Thirty oxosteroids were analysed after derivatisation with TMTH by electrospray ionization mass spectrometry (ESI-MS) and were found to give high ion-currents compared to underivatised molecules. ESI-tandem mass spectrometry (MS/MS) analysis of the derivatives yielded characteristic fragmentation patterns with specific mass reporter ions derived from the TMT group. A shotgun ESI-MS method incorporating TMTH derivatisation was applied to a urine sample |
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| College: |
Faculty of Medicine, Health and Life Sciences |